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Citations to this article

Dynamic changes in fat oxidation in human primary myocytes mirror metabolic characteristics of the donor
Barbara Ukropcova, … , George A. Bray, Steven R. Smith
Barbara Ukropcova, … , George A. Bray, Steven R. Smith
Published July 1, 2005
Citation Information: J Clin Invest. 2005;115(7):1934-1941. https://doi.org/10.1172/JCI24332.
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Research Article Metabolism Article has an altmetric score of 25

Dynamic changes in fat oxidation in human primary myocytes mirror metabolic characteristics of the donor

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Abstract

Metabolic flexibility of skeletal muscle, that is, the preference for fat oxidation (FOx) during fasting and for carbohydrate oxidation in response to insulin, is decreased during insulin resistance. The aim of this study was to test the hypothesis that the capacity of myotubes to oxidize fat in vitro reflects the donor’s metabolic characteristics. Insulin sensitivity (IS) and metabolic flexibility of 16 healthy, young male subjects was determined by euglycemic hyperinsulinemic clamp. Muscle samples were obtained from vastus lateralis, cultured, and differentiated into myotubes. In human myotubes in vitro, we measured suppressibility (glucose suppression of FOx) and adaptability (an increase in FOx in the presence of high palmitate concentration). We termed these dynamic changes in FOx metabolic switching. In vivo, metabolic flexibility was positively correlated with IS and maximal oxygen uptake and inversely correlated with percent body fat. In vitro suppressibility was inversely correlated with IS and metabolic flexibility and positively correlated with body fat and fasting FFA levels. Adaptability was negatively associated with percent body fat and fasting insulin and positively correlated with IS and metabolic flexibility. The interindividual variability in metabolic phenotypes was preserved in human myotubes separated from their neuroendocrine environment, which supports the hypothesis that metabolic switching is an intrinsic property of skeletal muscle.

Authors

Barbara Ukropcova, Michele McNeil, Olga Sereda, Lilian de Jonge, Hui Xie, George A. Bray, Steven R. Smith

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Total citations by year

Year: 2025 2024 2023 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Total
Citations: 4 3 1 4 5 3 3 3 4 6 4 6 5 13 9 4 10 2 3 1 93
Citation information
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Citations to this article in year 2015 (6)

Title and authors Publication Year
Nitrate enhances skeletal muscle fatty acid oxidation via a nitric oxide-cGMP-PPAR-mediated mechanism
T Ashmore, LD Roberts, AJ Morash, AO Kotwica, J Finnerty, JA West, SA Murfitt, BO Fernandez, C Branco, AS Cowburn, K Clarke, RS Johnson, M Feelisch, JL Griffin, AJ Murray
BMC Biology 2015
Myotubes from Severely Obese Type 2 Diabetic Subjects Accumulate Less Lipids and Show Higher Lipolytic Rate than Myotubes from Severely Obese Non-Diabetic Subjects
SS Bakke, YZ Feng, N Nikolić, ET Kase, C Moro, C Stensrud, L Damlien, MO Ludahl, R Sandbu, BM Solheim, AC Rustan, J Hjelmesæth, GH Thoresen, V Aas, G Sesti
PloS one 2015
Effect of a 1-week, eucaloric, moderately high-fat diet on peripheral insulin sensitivity in healthy premenopausal women
NM Branis, M Etesami, RW Walker, ES Berk, JB Albu
BMJ Open Diabetes Research & Care 2015
Perilipin 3 Differentially Regulates Skeletal Muscle Lipid Oxidation in Active, Sedentary, and Type 2 Diabetic Males
JD Covington, RC Noland, RC Hebert, BS Masinter, SR Smith, AC Rustan, E Ravussin, S Bajpeyi
The Journal of clinical endocrinology and metabolism 2015
High respiratory quotient is associated with increases in body weight and fat mass in young adults
RP Shook, GA Hand, AE Paluch, X Wang, R Moran, JR Hébert, JM Jakicic, SN Blair
European Journal of Clinical Nutrition 2015
Adipogenic progenitors from obese human skeletal muscle give rise to functional white adipocytes that contribute to insulin resistance
C Laurens, K Louche, C Sengenes, M Coué, D Langin, C Moro, V Bourlier
International Journal of Obesity 2015

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