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Citations to this article

Dynamic changes in fat oxidation in human primary myocytes mirror metabolic characteristics of the donor
Barbara Ukropcova, … , George A. Bray, Steven R. Smith
Barbara Ukropcova, … , George A. Bray, Steven R. Smith
Published July 1, 2005
Citation Information: J Clin Invest. 2005;115(7):1934-1941. https://doi.org/10.1172/JCI24332.
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Research Article Metabolism Article has an altmetric score of 25

Dynamic changes in fat oxidation in human primary myocytes mirror metabolic characteristics of the donor

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Abstract

Metabolic flexibility of skeletal muscle, that is, the preference for fat oxidation (FOx) during fasting and for carbohydrate oxidation in response to insulin, is decreased during insulin resistance. The aim of this study was to test the hypothesis that the capacity of myotubes to oxidize fat in vitro reflects the donor’s metabolic characteristics. Insulin sensitivity (IS) and metabolic flexibility of 16 healthy, young male subjects was determined by euglycemic hyperinsulinemic clamp. Muscle samples were obtained from vastus lateralis, cultured, and differentiated into myotubes. In human myotubes in vitro, we measured suppressibility (glucose suppression of FOx) and adaptability (an increase in FOx in the presence of high palmitate concentration). We termed these dynamic changes in FOx metabolic switching. In vivo, metabolic flexibility was positively correlated with IS and maximal oxygen uptake and inversely correlated with percent body fat. In vitro suppressibility was inversely correlated with IS and metabolic flexibility and positively correlated with body fat and fasting FFA levels. Adaptability was negatively associated with percent body fat and fasting insulin and positively correlated with IS and metabolic flexibility. The interindividual variability in metabolic phenotypes was preserved in human myotubes separated from their neuroendocrine environment, which supports the hypothesis that metabolic switching is an intrinsic property of skeletal muscle.

Authors

Barbara Ukropcova, Michele McNeil, Olga Sereda, Lilian de Jonge, Hui Xie, George A. Bray, Steven R. Smith

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Total citations by year

Year: 2025 2024 2023 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Total
Citations: 4 3 1 4 5 3 3 3 4 6 4 6 5 13 9 4 10 2 3 1 93
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2010 (9)

Title and authors Publication Year
Metabolic switching of human myotubes is improved by n-3 fatty acids
NP Hessvik, SS Bakke, K Fredriksson, MV Boekschoten, A Fjørkenstad, G Koster, MK Hesselink, S Kersten, ET Kase, AC Rustan, GH Thoresen
Journal of lipid research 2010
Peroxisome Proliferator-Activated Receptor-  Coactivator-1  Overexpression Increases Lipid Oxidation in Myocytes From Extremely Obese Individuals
LA Consitt, JA Bell, TR Koves, DM Muoio, MW Hulver, KR Haynie, GL Dohm, JA Houmard
2010
Skeletal muscle insulin resistance: the interplay of local lipid excess and mitochondrial dysfunction
L Chow, A From, E Seaquist
Metabolism: clinical and experimental 2010
Oxidative status of muscle is determined by p107 regulation of PGC-1 
A Scimè, VD Soleimani, CF Bentzinger, MA Gillespie, FL Grand, G Grenier, L Bevilacqua, ME Harper, MA Rudnicki
The Journal of Cell Biology 2010
Sex steroids affect triglyceride handling, glucose-dependent insulinotropic polypeptide, and insulin sensitivity: a 1-week randomized clinical trial in healthy young men
B Lapauw, M Ouwens, LM Hart, B Wuyts, JJ Holst, G T'Sjoen, JM Kaufman, JB Ruige
Diabetes care 2010
Intramuscular triacylglycerol and insulin resistance: Guilty as charged or wrongly accused?
DM Muoio
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 2010
Regulation of Skeletal Muscle Oxidative Capacity and Insulin Signaling by the Mitochondrial Rhomboid Protease PARL
AE Civitarese, PS MacLean, S Carling, L Kerr-Bayles, RP McMillan, A Pierce, TC Becker, C Moro, J Finlayson, N Lefort, CB Newgard, L Mandarino, W Cefalu, K Walder, GR Collier, MW Hulver, SR Smith, E Ravussin
Cell Metabolism 2010
Intramyocellular lipid accumulation is associated with permanent relocation ex vivo and in vitro of fatty acid translocase (FAT)/CD36 in obese patients
C Aguer, J Mercier, CY Man, L Metz, S Bordenave, K Lambert, E Jean, L Lantier, L Bounoua, JF Brun, ER de Mauverger, F Andreelli, M Foretz, M Kitzmann
Diabetologia 2010
LMNA Mutations, Skeletal Muscle Lipid Metabolism, and Insulin Resistance
M Boschmann, S Engeli, C Moro, A Luedtke, F Adams, K Gorzelniak, G Rahn, A Mähler, K Dobberstein, A Krüger, S Schmidt, S Spuler, FC Luft, SR Smith, HH Schmidt, J Jordan
The Journal of clinical endocrinology and metabolism 2010

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