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Citations to this article

An amino-bisphosphonate targets MMP-9–expressing macrophages and angiogenesis to impair cervical carcinogenesis
Enrico Giraudo, … , Masahiro Inoue, Douglas Hanahan
Enrico Giraudo, … , Masahiro Inoue, Douglas Hanahan
Published September 1, 2004
Citation Information: J Clin Invest. 2004;114(5):623-633. https://doi.org/10.1172/JCI22087.
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Article Oncology

An amino-bisphosphonate targets MMP-9–expressing macrophages and angiogenesis to impair cervical carcinogenesis

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Abstract

A mouse model involving the human papillomavirus type-16 oncogenes develops cervical cancers by lesional stages analogous to those in humans. In this study the angiogenic phenotype was characterized, revealing intense angiogenesis in high-grade cervical intraepithelial neoplasias (CIN-3) and carcinomas. MMP-9, a proangiogenic protease implicated in mobilization of VEGF, appeared in the stroma concomitant with the angiogenic switch, expressed by infiltrating macrophages, similar to what has been observed in humans. Preclinical trials sought to target MMP-9 and angiogenesis with a prototypical MMP inhibitor and with a bisphosphonate, zoledronic acid (ZA), revealing both to be antiangiogenic, producing effects comparable to a Mmp9 gene KO in impairing angiogenic switching, progression of premalignant lesions, and tumor growth. ZA therapy increased neoplastic epithelial and endothelial cell apoptosis without affecting hyperproliferation, indicating that ZA was not antimitotic. The analyses implicated cellular and molecular targets of ZA’s actions: ZA suppressed MMP-9 expression by infiltrating macrophages and inhibited metalloprotease activity, reducing association of VEGF with its receptor on angiogenic endothelial cells. Given its track record in clinical use with limited toxicity, ZA holds promise as an “unconventional” MMP-9 inhibitor for antiangiogenic therapy of cervical cancer and potentially for additional cancers and other diseases where MMP-9 expression by infiltrating macrophages is evident.

Authors

Enrico Giraudo, Masahiro Inoue, Douglas Hanahan

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Total
Citations: 6 9 10 15 15 10 10 9 12 10 18 19 23 22 23 20 15 10 7 8 1 272
Citation information
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Citations to this article in year 2018 (9)

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I Zuazo-Gaztelu, O Casanovas
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The reciprocal function and regulation of tumor vessels and immune cells offers new therapeutic opportunities in cancer
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Seminars in Cancer Biology 2018
Preclinical evaluation of an innovative anti-TAM approach based on zoledronate-loaded erythrocytes
R Sabatino, S Battistelli, M Magnani, L Rossi
Drug Delivery and Translational Research 2018
The Impact of Human Papilloma Viruses, Matrix Metallo-Proteinases and HIV Protease Inhibitors on the Onset and Progression of Uterine Cervix Epithelial Tumors: A Review of Preclinical and Clinical Studies
G Barillari, P Monini, C Sgadari, B Ensoli
International journal of molecular sciences 2018
High expression of CCL2 in tumor cells and abundant infiltration with CD14 positive macrophages predict early relapse in breast cancer
M Heiskala, M Leidenius, K Joensuu, P Heikkilä
Virchows Archiv 2018
Molecular Repolarisation of Tumour-Associated Macrophages
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Medically induced CSF rhinorrhea following treatment of macroprolactinoma: case series and literature review.
Česák T, Poczos P, Adamkov J, Náhlovský J, Kašparová P, Gabalec F, Čelakovský P, Choutka O
Pituitary 2018

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