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Citations to this article

Ex vivo analysis of human memory CD4 T cells specific for hepatitis C virus using MHC class II tetramers
Cheryl L. Day, … , Paul Klenerman, Kai W. Wucherpfennig
Cheryl L. Day, … , Paul Klenerman, Kai W. Wucherpfennig
Published September 15, 2003
Citation Information: J Clin Invest. 2003;112(6):831-842. https://doi.org/10.1172/JCI18509.
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Article Immunology Article has an altmetric score of 10

Ex vivo analysis of human memory CD4 T cells specific for hepatitis C virus using MHC class II tetramers

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Abstract

Containment of hepatitis C virus (HCV) and other chronic human viral infections is associated with persistence of virus-specific CD4 T cells, but ex vivo characterization of circulating CD4 T cells has not been achieved. To further define the phenotype and function of these cells, we developed a novel approach for the generation of tetrameric forms of MHC class II/peptide complexes that is based on the cellular peptide-exchange mechanism. HLA-DR molecules were expressed as precursors with a covalently linked CLIP peptide, which could be efficiently exchanged with viral peptides following linker cleavage. In subjects who spontaneously resolved HCV viremia, but not in those with chronic progressive infection, HCV tetramer–labeled cells could be isolated by magnetic bead capture despite very low frequencies (1:1,200 to 1:111,000) among circulating CD4 T cells. These T cells expressed a set of surface receptors (CCR7+CD45RA–CD27+) indicative of a surveillance function for secondary lymphoid structures and had undergone significant in vivo selection since they utilized a restricted Vβ repertoire. These studies demonstrate a relationship between clinical outcome and the presence of circulating CD4 T cells directed against this virus. Moreover, they show that rare populations of memory CD4 T cells can be studied ex vivo in human diseases.

Authors

Cheryl L. Day, Nilufer P. Seth, Michaela Lucas, Heiner Appel, Laurent Gauthier, Georg M. Lauer, Gregory K. Robbins, Zbigniew M. Szczepiorkowski, Deborah R. Casson, Raymond T. Chung, Shannon Bell, Gillian Harcourt, Bruce D. Walker, Paul Klenerman, Kai W. Wucherpfennig

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 Total
Citations: 1 2 5 4 6 7 4 3 9 6 10 5 9 10 5 9 6 9 11 9 5 7 1 143
Citation information
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Citations to this article in year 2017 (9)

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Nanomolar affinity anti-glycan antibody generation is controlled by T cells
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Journal of Clinical Investigation 2017
Innate and Adaptive Immune Responses in Chronic HCV Infection
LB Dustin
Current drug targets 2017
Evolution of MHC-based technologies used for detection of antigen-responsive T cells
AK Bentzen, SR Hadrup
Cancer Immunology, Immunotherapy 2017
An immunogenic personal neoantigen vaccine for patients with melanoma
PA Ott, Z Hu, DB Keskin, SA Shukla, J Sun, DJ Bozym, W Zhang, A Luoma, A Giobbie-Hurder, L Peter, C Chen, O Olive, TA Carter, S Li, DJ Lieb, T Eisenhaure, E Gjini, J Stevens, WJ Lane, I Javeri, K Nellaiappan, AM Salazar, H Daley, M Seaman, EI Buchbinder, CH Yoon, M Harden, N Lennon, S Gabriel, SJ Rodig, DH Barouch, JC Aster, G Getz, K Wucherpfennig, D Neuberg, J Ritz, ES Lander, EF Fritsch, N Hacohen, CJ Wu
Nature 2017
Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis
DA Rios, P Valva, PC Casciato, S Frias, MS Caldirola, MI Gaillard, L Bezrodnik, J Bandi, O Galdame, B Ameigeiras, D Krasniansky, C Brodersen, E Mullen, EN de Matteo, MV Preciado
Scientific Reports 2017
Fungus-Specific CD4 T Cells as Specific Sensors for Identification of Pulmonary Fungal Infections
A Scheffold, C Schwarz, P Bacher
Mycopathologia 2017
Guidelines for the use of flow cytometry and cell sorting in immunological studies: Guidelines for the use of flow cytometry and cell sorting in immunological studies
A Cossarizza, HD Chang, A Radbruch, M Akdis, I Andrä, F Annunziato, P Bacher, V Barnaba, L Battistini, WM Bauer, S Baumgart, B Becher, W Beisker, C Berek, A Blanco, G Borsellino, PE Boulais, RR Brinkman, M Büscher, DH Busch, TP Bushnell, X Cao, A Cavani, PK Chattopadhyay, Q Cheng, S Chow, M Clerici, A Cooke, A Cosma, L Cosmi, A Cumano, VD Dang, D Davies, SD Biasi, GD Zotto, SD Bella, P Dellabona, G Deniz, M Dessing, A Diefenbach, JD Santo, F Dieli, A Dolf, VS Donnenberg, T Dörner, GR Ehrhardt, E Endl, P Engel, B Engelhardt, C Esser, B Everts, A Dreher, CS Falk, TA Fehniger, A Filby, S Fillatreau, M Follo, I Förster, J Foster, GA Foulds, PS Frenette, D Galbraith, N Garbi, MD García-Godoy, J Geginat, K Ghoreschi, L Gibellini, C Goettlinger, CS Goodyear, A Gori, J Grogan, M Gross, A Grützkau, D Grummitt, J Hahn, Q Hammer, AE Hauser, DL Haviland, D Hedley, G Herrera, M Herrmann, F Hiepe, T Holland, P Hombrink, JP Houston, BF Hoyer, B Huang, CA Hunter, A Iannone, HM Jäck, B Jávega, S Jonjic, K Juelke, S Jung, T Kaiser, T Kalina, B Keller, S Khan, D Kienhöfer, T Kroneis, D Kunkel, C Kurts, P Kvistborg, J Lannigan, O Lantz, A Larbi, S LeibundGut-Landmann, MD Leipold, MK Levings, V Litwin, Y Liu, M Lohoff, G Lombardi, L Lopez, A Lovett-Racke, E Lubberts, B Ludewig, E Lugli, HT Maecker, G Martrus, G Matarese, C Maueröder, M McGrath, I McInnes, HE Mei, F Melchers, S Melzer, D Mielenz, K Mills, D Mirrer, J Mjösberg, J Moore, B Moran, A Moretta, L Moretta, TR Mosmann, S Müller, W Müller, C Münz, G Multhoff, LE Munoz, KM Murphy, T Nakayama, M Nasi, C Neudörfl, J Nolan, S Nourshargh, JE O'Connor, W Ouyang, A Oxenius, R Palankar, I Panse, P Peterson, C Peth, J Petriz, D Philips, W Pickl, S Piconese, M Pinti, AG Pockley, MJ Podolska, C Pucillo, SA Quataert, TR Radstake, B Rajwa, JA Rebhahn, D Recktenwald, EB Remmerswaal, K Rezvani, LG Rico, JP Robinson, C Romagnani, A Rubartelli, B Ruckert, J Ruland, S Sakaguchi, F Sala-de-Oyanguren, Y Samstag, S Sanderson, B Sawitzki, A Scheffold, M Schiemann, F Schildberg, E Schimisky, SA Schmid, S Schmitt, K Schober, T Schüler, AR Schulz, T Schumacher, C Scotta, TV Shankey, A Shemer, AK Simon, J Spidlen, AM Stall, R Stark, C Stehle, M Stein, T Steinmetz, H Stockinger, Y Takahama, A Tarnok, ZG Tian, G Toldi, J Tornack, E Traggiai, J Trotter, H Ulrich, M der Braber, RA van Lier, M Veldhoen, S Vento-Asturias, P Vieira, D Voehringer, HD Volk, K Volkmann, A Waisman, R Walker, MD Ward, K Warnatz, S Warth, JV Watson, C Watzl, L Wegener, A Wiedemann, J Wienands, G Willimsky, J Wing, P Wurst, L Yu, A Yue, Q Zhang, Y Zhao, S Ziegler, J Zimmermann
European Journal of Immunology 2017
CD4 + T Cell Differentiation in Chronic Viral Infections: The Tfh Perspective
LA Vella, RS Herati, EJ Wherry
Trends in Molecular Medicine 2017
Antigen presentation profiling reveals recognition of lymphoma immunoglobulin neoantigens
MS Khodadoust, N Olsson, LE Wagar, OA Haabeth, B Chen, K Swaminathan, K Rawson, CL Liu, D Steiner, P Lund, S Rao, L Zhang, C Marceau, H Stehr, AM Newman, DK Czerwinski, VE Carlton, M Moorhead, M Faham, HE Kohrt, J Carette, MR Green, MM Davis, R Levy, JE Elias, AA Alizadeh
Nature 2017

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