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The forkhead transcription factor Foxo1 links insulin signaling to Pdx1 regulation of pancreatic β cell growth
Tadahiro Kitamura, … , Karen C. Arden, Domenico Accili
Tadahiro Kitamura, … , Karen C. Arden, Domenico Accili
Published December 15, 2002
Citation Information: J Clin Invest. 2002;110(12):1839-1847. https://doi.org/10.1172/JCI16857.
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Article Metabolism

The forkhead transcription factor Foxo1 links insulin signaling to Pdx1 regulation of pancreatic β cell growth

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Abstract

Research Article

Authors

Tadahiro Kitamura, Jun Nakae, Yukari Kitamura, Yoshiaki Kido, William H. Biggs III, Christopher V.E. Wright, Morris F. White, Karen C. Arden, Domenico Accili

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Figure 5

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(a) Pdx1 expression in βTC-3 cells transfected with c-Myc–tagged wild-ty...
(a) Pdx1 expression in βTC-3 cells transfected with c-Myc–tagged wild-type (left column) or ADA-Foxo1 (right column). We performed immunocytochemistry with anti–c-Myc (top row) and anti-Pdx1 (middle row). We visualized anti–c-Myc immunoreactivity with CY3-conjugated anti-mouse IgG (red) and anti-Pdx1 immunoreactivity with FITC-conjugated anti-rabbit IgG (green). We show double staining in the bottom row. (b) Foxo1 localization in pancreatic duct–associated cells. We treated frozen sections for immunohistochemistry with anti-insulin and anti-Pdx1 antisera, followed by incubation in X-gal to detect β-gal expression from the Foxo1 locus. In the top row, we show a representative endocrine islet, demonstrating that X-gal reactivity colocalizes with islets. Occasional X-gal–positive cells can also be visualized in ducts and acini. In the middle and bottom rows, we show that X-gal reactivity in ducts can be detected in cells that do not express insulin and/or Pdx1 (red arrows), as well as cells with insulin and Pdx1 immunoreactivity (black arrows).

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