(A) Frequency of CD3⁺, CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells in the lungs of unvaccinated and BCG-vaccinated CC mice 4 weeks after infection. (B) Proportion of memory CD4 and CD8 T cells in the lungs of either unvaccinated or BCG-vaccinated CC mice 4 weeks after infection, as defined by CD44⁺ and various combinations of CD62L and CD127 expression; CD62L⁺CD127⁺ (central memory), CD62L^–CD127^– (effector), CD62L^–CD127⁺ (effector memory). (C) Frequency of resident memory CD4 and CD8 T cells in the lungs of either unvaccinated or BCG-vaccinated CC mice 4 weeks after infection, as defined by CD44⁺CD103⁺CD69⁺. (D) Proportion of Th1, Treg and Th17 cells in the lungs of either unvaccinated or BCG-vaccinated CC mice 4 weeks after infection, as defined by CD44⁺ and Tbet⁺ (Th1), Foxp3⁺ (Treg) or RoRγt (Th17) expression. (E) Frequency of CD4 and CD8 T cells expressing either CXCR3 or CX₃CR1 in the lungs of either unvaccinated or BCG-vaccinated CC mice 4 weeks after infection. (F) PCA of the T cell–phenotyping data. Each point represents the average of 5 mice within a given mouse strain that are either BCG vaccinated or unvaccinated. Open symbols, unvaccinated; closed symbols, BCG vaccinated. Each color represents a different mouse strain. The unvaccinated symbol is labeled with the CC strain name. The legend is modeled after the quadrants in Figure 1C. (A–E) The data represent the mean ± SEM from 1 experiment (n = 5/group). 2-way ANOVA with the Benjamini and Hochberg multiple comparison method. The FDR was set to 0.05 and the numbers in the figures are the q value. Quantitative data in panels A, C, and E are represented as box-and-whisker plots, with bounds from 25th to 75th percentile, median line and whiskers ranging from minimum to maximum.