PPARγ ligands inhibit in vivo angiogenesis in the CAM and bFGF-induced corneal neovascularization. (a) A normal CAM implanted with a methylcellulose disk. (b) After a 48-hour exposure to rosiglitazone (2.5 μg), avascular zones free of capillaries and small vessels were observed around the disc (arrows point to a 3-mm avascular zone). Scale bar, 22.5 mm. (c) bFGF-induced neovascularization in control cornea on day 6. (d–f) Systemic treatment with rosiglitazone at 50 mg/kg/d (d), 100 mg/kg/d (e), and 400 mg/kg/d (f). (g) Area of inhibition (percent) by various doses of daily and twice-daily rosiglitazone administration. Inhibition was determined on day 6 by the formula 0.2 × π × neovessel length × clock hours of neovessels (n = 6 eyes per group; experiment was performed three times). Lines are fitted to the data assuming a Gaussian distribution.