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Vitamin E inhibits CD95 ligand expression and protects T cells from activation-induced cell death
Min Li-Weber, … , Raoul Breitkreutz, Peter H. Krammer
Min Li-Weber, … , Raoul Breitkreutz, Peter H. Krammer
Published September 1, 2002
Citation Information: J Clin Invest. 2002;110(5):681-690. https://doi.org/10.1172/JCI15073.
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Article Genetics

Vitamin E inhibits CD95 ligand expression and protects T cells from activation-induced cell death

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Abstract

Research Article

Authors

Min Li-Weber, Markus A. Weigand, Marco Giaisi, Dorothee Süss, Monika K. Treiber, Sven Baumann, Elena Ritsou, Raoul Breitkreutz, Peter H. Krammer

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Figure 4

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Vitamin E blocks binding of NF-κB and AP-1 to the CD95L promoter target ...
Vitamin E blocks binding of NF-κB and AP-1 to the CD95L promoter target sites. (a) 32P-labeled oligonucleotides containing the CD95L NF-κB (at the promoter –530 and –50 region) and AP-1 (at the promoter +90 region) binding sequences were analyzed by EMSA. Nuclear extracts were prepared from J-27 T cells either not induced (–) or induced (+) by PMA and ionomycin in the absence (–) or presence (+) of vitamin E. The inducible complexes corresponding to NF-κB and AP-1 are indicated. An NF-Y binding oligonucleotide was used for equal loading of nuclear extracts. (b) EMSA was carried out in the absence (–) or presence (+) of the antibodies indicated.

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