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Citations to this article

Loss of glomerular foot processes is associated with uncoupling of podocalyxin from the actin cytoskeleton
Tetsuro Takeda, … , Robert A. Orlando, Marilyn G. Farquhar
Tetsuro Takeda, … , Robert A. Orlando, Marilyn G. Farquhar
Published July 15, 2001
Citation Information: J Clin Invest. 2001;108(2):289-301. https://doi.org/10.1172/JCI12539.
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Article Article has an altmetric score of 9

Loss of glomerular foot processes is associated with uncoupling of podocalyxin from the actin cytoskeleton

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Abstract

Podocalyxin (PC), the major sialoprotein of glomerular epithelial cells (GECs), helps maintain the characteristic architecture of the foot processes and the patency of the filtration slits. PC associates with actin via ezrin, a member of the ERM family of cytoskeletal linker proteins. Here we show that PC is linked to ezrin and the actin cytoskeleton via Na+/H+-exchanger regulatory factor 2 (NHERF2), a scaffold protein containing two PDZ (PSD-95/Dlg/ZO-1) domains and an ERM-binding region. The cytoplasmic tail of PC contains a C-terminal PDZ-binding motif (DTHL) that binds to the second PDZ domain of NHERF2 in yeast two-hybrid and in vitro pull-down assays. By immunocytochemistry NHERF2 colocalizes with PC and ezrin along the apical domain of the GEC plasma membrane. NHERF2 and ezrin form a multimeric complex with PC, as they coimmunoprecipitate with PC. The PC/NHERF2/ezrin complex interacts with the actin cytoskeleton, and this interaction is disrupted in GECs from puromycin aminonucleoside–, protamine sulfate–, or sialidase-treated rats, which show a dramatic loss of foot processes, comparable to that seen in the nephrotic syndrome. Thus NHERF2 appears to function as a scaffold protein linking PC to ezrin and the actin cytoskeleton. PC/NHERF2/ezrin/actin interactions are disrupted in pathologic conditions associated with changes in GEC foot processes, indicating their importance for maintaining the unique organization of this epithelium.

Authors

Tetsuro Takeda, Tammie McQuistan, Robert A. Orlando, Marilyn G. Farquhar

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Total citations by year

Year: 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2001 Total
Citations: 2 3 1 3 3 4 9 9 11 5 5 6 10 10 5 9 3 6 1 3 1 3 2 114
Citation information
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Citations to this article in year 2007 (6)

Title and authors Publication Year
Mutation in the key enzyme of sialic acid biosynthesis causes severe glomerular proteinuria and is rescued by N-acetylmannosamine
Belinda Galeano, Riko Klootwijk, Irini Manoli, MaoSen Sun, Carla Ciccone, Daniel Darvish, Matthew F. Starost, Patricia M. Zerfas, Victoria J. Hoffmann, Shelley Hoogstraten-Miller, Donna M. Krasnewich, William A. Gahl, Marjan Huizing
Journal of Clinical Investigation 2007
Sizing up sialic acid in glomerular disease
Susan E. Quaggin
Journal of Clinical Investigation 2007
Dual effects of RAS blockade on blood pressure and podocyte function
J Reiser, P Mundel
Current Hypertension Reports 2007
The CD34-related molecule podocalyxin is a potent inducer of microvillus formation
JS Nielsen, ML Graves, S Chelliah, AW Vogl, CD Roskelley, KM McNagny
PloS one 2007
Synaptopodin protects against proteinuria by disrupting Cdc42:IRSp53:Mena signaling complexes in kidney podocytes
E Yanagida-Asanuma, K Asanuma, K Kim, M Donnelly, HY Choi, JH Chang, S Suetsugu, Y Tomino, T Takenawa, C Faul, P Mundel
The American Journal of Pathology 2007
A Bipartite Signal Regulates the Faithful Delivery of Apical Domain Marker Podocalyxin/Gp135
CY Yu, JY Chen, YY Lin, KF Shen, WL Lin, CL Chien, MB ter Beest, TS Jou, H Riezman
Molecular biology of the cell 2007

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