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Citations to this article

Temporal dynamics of Wnt-dependent transcriptome reveal an oncogenic Wnt/MYC/ribosome axis
Babita Madan, … , Enrico Petretto, David M. Virshup
Babita Madan, … , Enrico Petretto, David M. Virshup
Published October 9, 2018
Citation Information: J Clin Invest. 2018;128(12):5620-5633. https://doi.org/10.1172/JCI122383.
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Research Article Cell biology Oncology Article has an altmetric score of 9

Temporal dynamics of Wnt-dependent transcriptome reveal an oncogenic Wnt/MYC/ribosome axis

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Abstract

Activating mutations in the Wnt pathway drive a variety of cancers, but the specific targets and pathways activated by Wnt ligands are not fully understood. To bridge this knowledge gap, we performed a comprehensive time-course analysis of Wnt-dependent signaling pathways in an orthotopic model of Wnt-addicted pancreatic cancer, using a porcupine (PORCN) inhibitor currently in clinical trials, and validated key results in additional Wnt-addicted models. The temporal analysis of the drug-perturbed transcriptome demonstrated direct and indirect regulation of more than 3,500 Wnt-activated genes (23% of the transcriptome). Regulation was both via Wnt/β-catenin and through the modulation of protein abundance of important transcription factors, including MYC, via Wnt-dependent stabilization of proteins (Wnt/STOP). Our study identifies a central role of Wnt/β-catenin and Wnt/STOP signaling in controlling ribosome biogenesis, a key driver of cancer proliferation.

Authors

Babita Madan, Nathan Harmston, Gahyathiri Nallan, Alex Montoya, Peter Faull, Enrico Petretto, David M. Virshup

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Total citations by year

Year: 2024 2023 2022 2021 2020 2019 Total
Citations: 9 4 7 11 6 4 41
Citation information
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Citations to this article (41)

Title and authors Publication Year
The cholesterol biosynthetic enzyme FAXDC2 couples Wnt/β-catenin to RTK/MAPK signaling
Babita Madan, Shawn Wadia, Siddhi Patnaik, Nathan Harmston, Emile Tan, Iain Tan, David Nes, Enrico Petretto, David Virshup
Journal of Clinical Investigation 2024
METTL3 promotes osteoblast ribosome biogenesis and alleviates periodontitis.
Zhang Y, Kong Y, Zhang W, He J, Zhang Z, Cai Y, Zhao Y, Xu Q
Clinical Epigenetics 2024
Protein translation: biological processes and therapeutic strategies for human diseases.
Jia X, He X, Huang C, Li J, Dong Z, Liu K
Signal Transduction and Targeted Therapy 2024
Evaluation of Microstructure and Abrasive Wear-Resistance of Medium Alloy SiMo Ductile Cast Iron
Dyrlaga Ł, Zapała R, Morgiel K, Studnicki A, Szczęsny A, Kopyciński D
Materials 2024
Clinical and Preclinical Targeting of Oncogenic Pathways in PDAC: Targeted Therapeutic Approaches for the Deadliest Cancer
Jiménez DJ, Javed A, Rubio-Tomás T, Seye-Loum N, Barceló C
International journal of molecular sciences 2024
Recurrent mutations in tumor suppressor FBXW7 bypass Wnt/β-catenin addiction in cancer.
Zhong Z, Virshup DM
Science Advances 2024
Developmental signals control chromosome segregation fidelity during pluripotency and neurogenesis by modulating replicative stress
de Jaime-Soguero A, Hattemer J, Bufe A, Haas A, van den Berg J, van Batenburg V, Das B, di Marco B, Androulaki S, Böhly N, Landry JJ, Schoell B, Rosa VS, Villacorta L, Baskan Y, Trapp M, Benes V, Chabes A, Shahbazi M, Jauch A, Engel U, Patrizi A, Sotillo R, van Oudenaarden A, Bageritz J, Alfonso J, Bastians H, Acebrón SP
Nature Communications 2024
The RNA helicase DDX21 activates YAP to promote tumorigenesis and is transcriptionally upregulated by β-catenin in colorectal cancer.
Tang W, Yang Y, Fu Z, Xu W, Ou W, Liu F, Du P, Liu CY
Oncogene 2024
Isoleucyl-tRNA synthetase 2 promotes pancreatic ductal adenocarcinoma proliferation and metastasis by stabilizing β-catenin
Jin Y, Huang X, Wang Z, Kouken B, Wang Q, Wang L
Genes & Diseases 2024
The Roles of Secreted Wnt Ligands in Cancer
Werner J, Boonekamp KE, Zhan T, Boutros M
International journal of molecular sciences 2023
The Role of WNT Pathway Mutations in Cancer Development and an Overview of Therapeutic Options
Groenewald W, Lund AH, Gay DM
Cells 2023
The role of Evi/Wntless in exporting Wnt proteins
Wolf L, Boutros M
Development (Cambridge, England) 2023
Experimental Murine Models for Colorectal Cancer Research.
Neto Í, Rocha J, Gaspar MM, Reis CP
Cancers 2023
A p300/GATA6 axis determines differentiation and Wnt dependency in pancreatic cancer models
Zheng Zhong, Nathan Harmston, Kris C. Wood, Babita Madan, David M. Virshup
Journal of Clinical Investigation 2022
TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis
Y Wang, Y Chen, C Li, Z Xiao, H Yuan, Y Zhang, D Pang, X Tang, M Li, H Ouyang
Biology : open access journal 2022
Target c-Myc to treat pancreatic cancer
M Ala
Cancer biology & therapy 2022
mRNAsi-related genes can effectively distinguish hepatocellular carcinoma into new molecular subtypes.
Wang C, Qin S, Pan W, Shi X, Gao H, Jin P, Xia X, Ma F
Computational and Structural Biotechnology Journal 2022
Porcupine inhibition disrupts mitochondrial function and homeostasis in WNT ligand-addicted pancreatic cancer
Aguilera KY, Le T, Riahi R, Lay AR, Hinz S, Saadat EA, Vashisht AA, Wohlschlegel J, Donahue TR, Radu CG, Dawson DW
Molecular cancer therapeutics 2022
Regulation of SUMOylation Targets Associated With Wnt/β-Catenin Pathway.
Fan L, Yang X, Zheng M, Yang X, Ning Y, Gao M, Zhang S
Frontiers in Oncology 2022
Multi-transcriptome analysis following an acute skeletal muscle growth stimulus yields tools for discerning global and MYC regulatory networks
Murach KA, Liu Z, Jude B, Figueiredo VC, Wen Y, Khadgi S, Lim S, Morena da Silva F, Greene NP, Lanner JT, McCarthy JJ, Vechetti IJ, von Walden F
The Journal of biological chemistry 2022
WNT inhibition creates a BRCA‐like state in Wnt‐addicted cancer
A Kaur, JY Lim, S Sepramaniam, S Patnaik, N Harmston, MA Lee, E Petretto, DM Virshup, B Madan
EMBO Molecular Medicine 2021
Inhibiting β-catenin disables nucleolar functions in triple-negative breast cancer
SE Weeks, SC Kammerud, BJ Metge, HA AlSheikh, DA Schneider, D Chen, S Wei, JA Mobley, AI Ojesina, LA Shevde, RS Samant
Cell Death and Disease 2021
Genome-scale CRISPR-Cas9 screen of Wnt/β-catenin signaling identifies therapeutic targets for colorectal cancer
C Wan, S Mahara, C Sun, A Doan, HK Chua, D Xu, J Bian, Y Li, D Zhu, D Sooraj, T Cierpicki, J Grembecka, R Firestein
Science Advances 2021
Long noncoding RNA VPS9D1-AS1 promotes esophageal squamous cell carcinoma progression via the Wnt/β-catenin signaling pathway
L Ma, W Yan, X Sun, P Chen
Journal of Cancer 2021
C644‐0303, a small‐molecule inhibitor of the Wnt/β‐catenin pathway, suppresses colorectal cancer growth
Y Yan, Y Zhang, M Li, Y Zhang, X Zhang, X Zhang, Y Xu, W Wei, J Wang, X Xu, Q Song, C Zhao
Cancer Science 2021
Inhibition of the PLK1‐Coupled Cell Cycle Machinery Overcomes Resistance to Oxaliplatin in Colorectal Cancer
Z Yu, P Deng, Y Chen, S Liu, J Chen, Z Yang, J Chen, X Fan, P Wang, Z Cai, Y Wang, P Hu, D Lin, R Xiao, Y Zou, Y Huang, Q Yu, P Lan, J Tan, X Wu
Advanced Science 2021
Regulation of Wnt receptor activity: Implications for therapeutic development in colon cancer
ZA Zhong, MN Michalski, PD Stevens, EA Sall, BO Williams
The Journal of biological chemistry 2021
WNT Ligand Dependencies in Pancreatic Cancer
KY Aguilera, DW Dawson
Frontiers in Cell and Developmental Biology 2021
Oxidative damage-induced hyperactive ribosome biogenesis participates in tumorigenesis of offspring by cross-interacting with the Wnt and TGF-β1 pathways in IVF embryos
Y Huang, Z Li, E Lin, P He, G Ru
Experimental & molecular medicine 2021
Mitotic WNT signalling orchestrates neurogenesis in the developing neocortex
FD Silva, K Zhang, A Pinson, E Fatti, M WilschBräuninger, J Herbst, V Vidal, A Schedl, WB Huttner, C Niehrs
The EMBO Journal 2021
Wnt signaling recruits KIF2A to the spindle to ensure chromosome congression and alignment during mitosis
A Bufe, AG del Arco, M Hennecke, A de Jaime-Soguero, M Ostermaier, YC Lin, A Ciprianidis, J Hattemer, U Engel, P Beli, H Bastians, SP Acebrón
Proceedings of the National Academy of Sciences 2021
Wnts and the hallmarks of cancer
Z Zhong, J Yu, DM Virshup, B Madan
Cancer and Metastasis Reviews 2020
Wnt-regulated lncRNA discovery enhanced by in vivo identification and CRISPRi functional validation
S Liu, N Harmston, TL Glaser, Y Wong, Z Zhong, B Madan, DM Virshup, E Petretto
Genome Medicine 2020
APC loss induces Warburg effect via increased PKM2 transcription in colorectal cancer
PH Cha, JH Hwang, DK Kwak, E Koh, KS Kim, KY Choi
British Journal of Cancer 2020
RpS13 controls the homeostasis of germline stem cell niche through Rho1‐mediated signals in the Drosophila testis
M Wang, X Chen, Y Wu, Q Zheng, W Chen, Y Yan, X Luan, C Shen, J Fang, B Zheng, J Yu
Cell Proliferation 2020
Foxh1/Nodal Defines Context-Specific Direct Maternal Wnt/β-Catenin Target Gene Regulation in Early Development
BA Afouda, Y Nakamura, S Shaw, RM Charney, KD Paraiso, IL Blitz, KW Cho, S Hoppler
iScience 2020
Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer
C Morral, J Stanisavljevic, X Hernando-Momblona, E Mereu, A Álvarez-Varela, C Cortina, D Stork, F Slebe, G Turon, G Whissell, M Sevillano, A Merlos-Suárez, À Casanova-Martí, C Moutinho, S Lowe, L Dow, A Villanueva, E Sancho, H Heyn, E Batlle
Cell Stem Cell 2020
Broad regulation of gene isoform expression by Wnt signaling in cancer
M Idris, N Harmston, E Petretto, B Madan, DM Virshup
RNA (New York, N.Y.) 2019
WNT Signaling in Disease
Ng, Kaur, Bunnag, Suresh, Sung, Tan, Gruber, Tolwinski
Cells 2019
Wnt/β-Catenin Signaling: The Culprit in Pancreatic Carcinogenesis and Therapeutic Resistance
MR Makena, H Gatla, D Verlekar, S Sukhavasi, MK Pandey, KC Pramanik
International journal of molecular sciences 2019
PORCN inhibition synergizes with PI3K/mTOR inhibition in Wnt-addicted cancers
Z Zhong, S Sepramaniam, XH Chew, K Wood, MA Lee, B Madan, DM Virshup
Oncogene 2019

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