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Citations to this article

Expression of the Gs protein alpha-subunit disrupts the normal program of differentiation in cultured murine myogenic cells.
C C Tsai, … , J E Saffitz, J J Billadello
C C Tsai, … , J E Saffitz, J J Billadello
Published January 1, 1997
Citation Information: J Clin Invest. 1997;99(1):67-76. https://doi.org/10.1172/JCI119135.
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Research Article

Expression of the Gs protein alpha-subunit disrupts the normal program of differentiation in cultured murine myogenic cells.

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Abstract

The manner in which growth factors acting at the cell surface regulate activity of myogenic basic-helix-loop-helix proteins in the nucleus and thus control the fate of committed skeletal myoblasts remains poorly understood. In this study, we report that immunoreactive Gs protein alpha-subunits (Gs alpha) localize to nuclei of proliferating C2C12 myoblasts but not to nuclei of differentiated postmitotic C2C12 myotubes. To explore the biological significance of this observation, we placed a cDNA encoding Gs alpha in an expression vector under the control of a steroid-inducible promoter and isolated colonies of stably transfected C2C12 myoblasts. Dexamethasone-induced expression of activated Gs alpha markedly delayed differentiation in comparison with uninduced stably transfected cells, which differentiated normally in mitogen-depleted media. Northern blot analysis showed that impaired differentiation was associated with delayed up-regulation of MyoD and myogenin and delayed down-regulation of Id, a dominant negative inhibitor of differentiation. Similar impairment of differentiation could not be reproduced in wild-type C2C12 cells by increasing intracellular cAMP either with forskolin or treatment with a cell-permeable cAMP analog. However, treatment of myoblasts with cholera toxin markedly inhibited myogenic differentiation. Taken together, these findings suggest a novel role for Gs alpha in modulating myogenic differentiation.

Authors

C C Tsai, J E Saffitz, J J Billadello

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Total citations by year

Year: 2012 2011 2008 2005 2004 2000 1998 Total
Citations: 3 2 1 2 2 1 2 13
Citation information
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Citations to this article (13)

Title and authors Publication Year
Fibrous Dysplasia
P Bianco, S Wientroub
Pediatric Bone 2012
Pediatric Bone
M Bastepe, H Jüppner, RV Thakker
Pediatric Bone 2012
cAMP signaling in skeletal muscle adaptation: hypertrophy, metabolism, and regeneration
R Berdeaux, R Stewart
American journal of physiology. Endocrinology and metabolism 2012
Expression of Gαz in C2C12 cells restrains myogenic differentiation
H Mei, MK Ho, LY Yung, Z Wu, NY Ip, YH Wong
Cellular Signalling 2011
Cloning of the 5′ regulatory regions and functional characterization of the core promoters of ovine PLAU (u-PA) and SERPIN1 (PAI-1)
AD Lampidonis, G Theodorou, C Pecorini, R Rebucci, A Baldi, I Politis
Gene 2011
Localized cyclic AMP-dependent protein kinase activity is required for myogenic cell fusion
A Mukai, N Hashimoto
Experimental Cell Research 2008
Peptide and non-peptide G-protein coupled receptors (GPCRs) in skeletal muscle
G Jean-Baptiste, Z Yang, C Khoury, S Gaudio, MT Greenwood
Peptides 2005
β adrenergic receptor-mediated atrial specific up-regulation of RGS5
G Jean-Baptiste, XY Li, Z Yang, J Heubach, S Gaudio, C Khoury, U Ravens, MT Greenwood
Life Sciences 2005
Differentiation of H9c2 cardiomyoblasts: The role of adenylate cyclase system
M Pagano, S Naviglio, A Spina, E Chiosi, G Castoria, M Romano, A Sorrentino, F Illiano, G Illiano
Journal of Cellular Physiology 2004
Adenylate cyclase regulation via proteasome-mediated modulation of Gαs levels
S Naviglio, M Pagano, M Romano, A Sorrentino, A Fusco, F Illiano, E Chiosi, A Spina, G Illiano
Cellular Signalling 2004
Glucocorticoid inhibition of C2C12 proliferation rate and differentiation capacity in relation to mRNA levels of the MRF gene family
M F te Pas, P R de Jong, F J Verburg
Molecular Biology Reports 2000
Stimulatory Transducing Systems in Pancreatic Islet Cellsa
S Emami, K Regnauld, N Ferrand, A Astesano, M Pessah, H Phan, C Boissard, JM Garel, G Rosselin
Annals of the New York Academy of Sciences 1998
G Proteins, Receptors, and Disease
AM Spiegel
1998

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