Citations to this article

Conversion of amylase-secreting rat pancreatic AR42J cells to neuronlike cells by activin A.
H Ohnishi, … , O Shimada, H Ishikawa
H Ohnishi, … , O Shimada, H Ishikawa
Published May 1, 1995
Citation Information: J Clin Invest. 1995;95(5):2304-2314. https://doi.org/10.1172/JCI117922.
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Research Article

Conversion of amylase-secreting rat pancreatic AR42J cells to neuronlike cells by activin A.

Abstract

When AR42J cells, an amylase-secreting pancreatic exocrine cell line, were treated with activin A, cells extended neuritelike processes, and, concomitantly, amylase-containing vesicles disappeared. Immunofluorescence and immunoelectron microscopy revealed that these processes had neurite-specific cytoskeletal architectures: neurofilaments and microtubule bundles with cross-bridges of microtubule-associated protein 2. In addition to such morphological changes, activin-treated cells exhibited a marked increase in cytoplasmic free calcium concentration in response to depolarizing concentration of potassium. Moreover, activin-treated AR42J cells expressed mRNA for alpha 1 subunit of the neuroendocrine/beta cell-type voltage-dependent calcium channel. In naive AR42J cells, a sulfonylurea compound, tolbutamide, did not affect free calcium concentration, while it induced a marked elevation of free calcium in activin-treated cells. Single channel recording of the membrane patch revealed the existence of ATP-sensitive potassium channel in activin-treated cells. These results indicate that activin A converts amylase-secreting AR42J cells to neuronlike cells. Given that pancreatic endocrine cells possess neuronlike properties and express ATP-sensitive potassium channel as well as neuroendocrine/beta cell-type voltage-dependent calcium channel, activin treatment of AR42J cells may provide an in vitro model system to study the conversion of pancreatic exocrine cells to endocrine cells in islets.

Authors

H Ohnishi, N Ohgushi, S Tanaka, H Mogami, R Nobusawa, H Mashima, M Furukawa, T Mine, O Shimada, H Ishikawa

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Year: 2018 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1997 1996 Total
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Citations to this article (28)

Title and authors Publication Year
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Hepatocyte progenitors in man and in rodents – multiple pathways, multiple candidates
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H Mashima, N Ueda, H Ohno, J Suzuki, H Ohnishi, H Yasuda, T Tsuchida, C Kanamaru, N Makita, T Iiri, M Omata, I Kojima 		The Journal of biological chemistry 2003
Regulation of Pax4 Paired Homeodomain Gene by Neuron-restrictive Silencer Factor
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Expression of a Novel Zinc-Finger cDNA, IA-1, Is Associated with Rat AR42J Cells Differentiation into Insulin-Positive Cells
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Up-Regulation of the Expression of Activins in the Pancreatic Duct by Reduction of the β-Cell Mass
YQ Zhang, H Zhang, A Maeshima, H Kurihara, J Miyagawa, T Takeuchi, I Kojima 		Endocrinology 2002
Promotion of β-Cell Regeneration by Betacellulin in Ninety Percent-Pancreatectomized Rats
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Molecular Cloning of a Novel Brain-Type Na+-Dependent Inorganic Phosphate Cotransporter
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Betacellulin and activin A coordinately convert amylase-secreting pancreatic AR42J cells into insulin-secreting cells
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