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Citations to this article

Preferential activation of microsomal diacylglycerol/protein kinase C signaling during glucose treatment (De Novo phospholipid synthesis) of rat adipocytes.
R V Farese, … , H Mischak, D R Cooper
R V Farese, … , H Mischak, D R Cooper
Published May 1, 1994
Citation Information: J Clin Invest. 1994;93(5):1894-1899. https://doi.org/10.1172/JCI117180.
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Research Article

Preferential activation of microsomal diacylglycerol/protein kinase C signaling during glucose treatment (De Novo phospholipid synthesis) of rat adipocytes.

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Abstract

Glucose has been reported to increase the de novo synthesis of diacylglycerol (DAG) and translocate and activate protein kinase C (PKC) in rat adipocytes. Presently, we examined the major subcellular site of PKC translocation/activation in response to glucose-induced DAG. Glucose rapidly increased DAG content and PKC enzyme activity in microsomes, but not in plasma membranes or other membranes, during a 30-min treatment of rat adipocytes. This glucose-induced increase in microsomal DAG was attended by increases in immunoreactive PKC alpha, beta, and epsilon. Glucose-induced activation of DAG/PKC signaling in microsomes was not associated with a change in the translocation of Glut-4 transporters from microsomes to the plasma membrane, a biological response that is known to be stimulated by agonists, e.g., phorbol esters, which increase DAG/PKC signaling in plasma membranes, as well as in microsomes. In conclusion, an increase in de novo phospholipid synthesis, as occurs during glucose treatment of rat adipocytes, primarily activates DAG/PKC signaling in microsomes; moreover, this signaling response and biological consequences thereof may differ from those of agonists that primarily stimulate DAG/PKC signaling in the plasma membrane.

Authors

R V Farese, M L Standaert, T P Arnold, K Yamada, K Musunuru, H Hernandez, H Mischak, D R Cooper

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Total citations by year

Year: 2023 2021 2014 2010 2006 2001 2000 1999 1997 1996 1995 Total
Citations: 1 1 1 1 1 1 1 1 2 1 1 12
Citation information
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Citations to this article in year 2006 (1)

Title and authors Publication Year
High glucose protects embryonic cardiac cells against simulated ischemia
V Malliopoulou, C Xinaris, I Mourouzis, AD Cokkinos, N Katsilambros, C Pantos, E Kardami, DV Cokkinos
Molecular and Cellular Biochemistry 2006

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