Lupus autoantibodies to native DNA cross-react with the A and D SnRNP polypeptides.

M Reichlin; A Martin; E Taylor-Albert; K Tsuzaka; W Zhang; M W Reichlin; E Koren; F M Ebling; B Tsao; B H Hahn

doi:10.1172/JCI116980

Arthritis/Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104.

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Published in Volume 93, Issue 1 on January 1, 1994
J Clin Invest. 1994;93(1):443–449. https://doi.org/10.1172/JCI116980.
© 1994 The American Society for Clinical Investigation

Published January 1, 1994 - Version history

Antibodies to native DNA (nDNA) in sera from patients with systemic lupus erythematosus have been found to frequently correlate with antibodies to the A and D SnRNP proteins measured in Western blot assays. 40 of 54 SLE (74.1%) sera with anti-nDNA bound to A and D proteins, while 9 of 113 sera (8%) without anti-nDNA bound the A and D proteins, P < 10(-8) by Fisher's exact test. Antibodies to nDNA correlated closely with anti-A and anti-D in seven of eight patients followed sequentially, r = 0.7865. Nine human polyclonal anti-nDNA populations were isolated from DNA cellulose columns. Seven reacted equally with A and D, and two reacted predominantly with D. Two of three murine monoclonal anti-DNA antibodies isolated from NZB/NZW F1 hybrid mice bound A and D equally in Western blot with a titer > 1/40,000. These reactions were directed to the unfolded A and D proteins measurable in Western blot since these monoclonals (and several of the human anti-nDNA populations) failed to react with native U1RNP in ELISA or in RNA immunoprecipitation experiments. These newly recognized cross reactions of anti-nDNA may amplify the immune response to DNA and be part of the original immunogenic drive.

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Lupus autoantibodies to native DNA cross-react with the A and D SnRNP polypeptides.

M Reichlin, A Martin, E Taylor-Albert, K Tsuzaka, W Zhang, M W Reichlin, E Koren, F M Ebling, B Tsao, and B H Hahn

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Lupus autoantibodies to native DNA cross-react with the A and D SnRNP polypeptides.

M Reichlin, A Martin, E Taylor-Albert, K Tsuzaka, W Zhang, M W Reichlin, E Koren, F M Ebling, B Tsao, and B H Hahn

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