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Citations to this article

Epitope mapping of type VII collagen. Identification of discrete peptide sequences recognized by sera from patients with acquired epidermolysis bullosa.
J C Lapiere, … , A M Christiano, J Uitto
J C Lapiere, … , A M Christiano, J Uitto
Published October 1, 1993
Citation Information: J Clin Invest. 1993;92(4):1831-1839. https://doi.org/10.1172/JCI116774.
View: Text | PDF
Research Article

Epitope mapping of type VII collagen. Identification of discrete peptide sequences recognized by sera from patients with acquired epidermolysis bullosa.

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Abstract

Epidermolysis bullosa acquisita (EBA) is an acquired blistering skin disease characterized by the presence of IgG autoantibodies that recognize type VII (anchoring fibril) collagen. In this study, we have mapped the antigenic epitopes within the type VII collagen alpha chain by Western immunoblotting analysis with sera from 19 patients with EBA, using bacterial collagenase- or pepsin-resistant portions of type VII collagen and a panel of 12 recombinant fusion proteins corresponding to approximately 80% of the primary sequence of the alpha 1 (VII) collagen polypeptide. These studies identified four major immunodominant epitopes localized within the amino-terminal, noncollagenous (NC-1) domain. In addition to EBA, sera from three patients with bullous systemic lupus erythematosus (BSLE) were tested. The pattern of epitopes recognized by these sera were similar to those noted with EBA, suggesting that the same epitopes could serve as autoantigens in both blistering conditions. In contrast, sera from healthy controls or from patients with unrelated blistering skin diseases did not react with type VII collagen epitopes. Collectively, the results indicate that the immunodominant epitopes in EBA and BSLE lie within the noncollagenous regions of type VII collagen. The precise role of the circulating autoantibodies in the pathogenesis of these blistering diseases remains to be elucidated. Conceivably, however, such antibodies could disrupt the assembly of type VII collagen into anchoring fibrils and/or interfere with their interactions with other extracellular matrix molecules within the cutaneous basement membrane zone.

Authors

J C Lapiere, D T Woodley, M G Parente, T Iwasaki, K C Wynn, A M Christiano, J Uitto

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Total citations by year

Year: 2025 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2002 2001 2000 1999 1998 1997 1996 1995 1994 1986 1960 Total
Citations: 1 2 1 5 3 5 4 4 4 5 5 6 5 11 14 5 3 5 7 5 7 4 3 1 6 6 7 2 1 2 1 1 141
Citation information
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Citations to this article in year 2010 (14)

Title and authors Publication Year
Long-Term Type VII Collagen Restoration to Human Epidermolysis Bullosa Skin Tissue
Z Siprashvili, NT Nguyen, MY Bezchinsky, MP Marinkovich, AT Lane, PA Khavari
Human Gene Therapy 2010
Cross-reactivity of autoantibodies from patients with epidermolysis bullosa acquisita with murine collagen VII
K Csorba, A Sesarman, E Oswald, V Feldrihan, A Fritsch, T Hashimoto, C Sitaru
Cellular and Molecular Life Sciences 2010
Generation of Antibodies of Distinct Subclasses and Specificity Is Linked to H2s in an Active Mouse Model of Epidermolysis Bullosa Acquisita
RJ Ludwig, A Recke, K Bieber, S Müller, AC Marques, D Banczyk, M Hirose, M Kasperkiewicz, N Ishii, E Schmidt, J Westermann, D Zillikens, SM Ibrahim
Journal of Investigative Dermatology 2010
Epidermolysis bullosa acquisita: What’s new?
N ISHII, T HAMADA, T DAINICHI, T KARASHIMA, T NAKAMA, S YASUMOTO, D ZILLIKENS, T HASHIMOTO
The Journal of Dermatology 2010
Immune reactivity to type VII collagen: implications for gene therapy of recessive dystrophic epidermolysis bullosa
V Pendaries, G Gasc, M Titeux, C Leroux, ZG Vitezica, JE Mejía, A Décha, P Loiseau, C Bodemer, C Prost-Squarcioni, A Hovnanian
Gene Therapy 2010
Clearance rates of circulating and tissue-bound autoantibodies to type VII collagen in experimental epidermolysis bullosa acquisita
M Kasperkiewicz, M Hirose, A Recke, E Schmidt, D Zillikens, RJ Ludwig
British Journal of Dermatology 2010
T and B cells target identical regions of the non-collagenous domain 1 of type VII collagen in epidermolysis bullosa acquisita
R Müller, C Dahler, C Möbs, E Wenzel, R Eming, G Messer, A Niedermeier, M Hertl
Clinical Immunology 2010
Bullous Lupus: An Unusual Initial Presentation of Systemic Lupus Erythematosus in an Adolescent Girl
M Tincopa, KB Puttgen, S Sule, BA Cohen, MR Gerstenblith
Pediatric Dermatology 2010
Modern diagnosis of autoimmune blistering skin diseases
E Schmidt, D Zillikens
Autoimmunity Reviews 2010
Type VII collagen: the anchoring fibril protein at fault in dystrophic epidermolysis bullosa
HJ Chung, J Uitto
Dermatologic Clinics 2010
Rook's Textbook of Dermatology: Burns/Rook's Textbook of Dermatology
PS Friedmann, MR Ardern-Jones, CA Holden
Rook's Textbook of Dermatology: Burns/Rook's Textbook of Dermatology 2010
Épidermolyse bulleuse acquise
F Caux
La Presse Médicale 2010
Preventive Dermatology
R Norman
2010
Epitope Mapping of Antibodies Using a Cell Array–Based Polypeptide Library
RH Maier, CJ Maier, R Rid, H Hintner, JW Bauer, K Önder
Journal of biomolecular screening 2010

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