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Citations to this article

Epitope mapping of type VII collagen. Identification of discrete peptide sequences recognized by sera from patients with acquired epidermolysis bullosa.
J C Lapiere, … , A M Christiano, J Uitto
J C Lapiere, … , A M Christiano, J Uitto
Published October 1, 1993
Citation Information: J Clin Invest. 1993;92(4):1831-1839. https://doi.org/10.1172/JCI116774.
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Research Article

Epitope mapping of type VII collagen. Identification of discrete peptide sequences recognized by sera from patients with acquired epidermolysis bullosa.

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Abstract

Epidermolysis bullosa acquisita (EBA) is an acquired blistering skin disease characterized by the presence of IgG autoantibodies that recognize type VII (anchoring fibril) collagen. In this study, we have mapped the antigenic epitopes within the type VII collagen alpha chain by Western immunoblotting analysis with sera from 19 patients with EBA, using bacterial collagenase- or pepsin-resistant portions of type VII collagen and a panel of 12 recombinant fusion proteins corresponding to approximately 80% of the primary sequence of the alpha 1 (VII) collagen polypeptide. These studies identified four major immunodominant epitopes localized within the amino-terminal, noncollagenous (NC-1) domain. In addition to EBA, sera from three patients with bullous systemic lupus erythematosus (BSLE) were tested. The pattern of epitopes recognized by these sera were similar to those noted with EBA, suggesting that the same epitopes could serve as autoantigens in both blistering conditions. In contrast, sera from healthy controls or from patients with unrelated blistering skin diseases did not react with type VII collagen epitopes. Collectively, the results indicate that the immunodominant epitopes in EBA and BSLE lie within the noncollagenous regions of type VII collagen. The precise role of the circulating autoantibodies in the pathogenesis of these blistering diseases remains to be elucidated. Conceivably, however, such antibodies could disrupt the assembly of type VII collagen into anchoring fibrils and/or interfere with their interactions with other extracellular matrix molecules within the cutaneous basement membrane zone.

Authors

J C Lapiere, D T Woodley, M G Parente, T Iwasaki, K C Wynn, A M Christiano, J Uitto

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Total citations by year

Year: 2025 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2002 2001 2000 1999 1998 1997 1996 1995 1994 1986 1960 Total
Citations: 1 2 1 5 3 5 4 4 4 5 5 6 5 11 14 5 3 5 7 5 7 4 3 1 6 6 7 2 1 2 1 1 141
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Citations to this article in year 2009 (5)

Title and authors Publication Year
T cells are required for the production of blister-inducing autoantibodies in experimental epidermolysis bullosa acquisita
AG Sitaru, A Sesarman, S Mihai, MT Chiriac, D Zillikens, P Hultman, W Solbach, C Sitaru
Journal of immunology (Baltimore, Md. : 1950) 2009
Pathogenicity of IgG subclass autoantibodies to type VII collagen: induction of dermal-epidermal separation
A Recke, C Sitaru, G Vidarsson, M Evensen, MT Chiriac, RJ Ludwig, D Zillikens
Journal of Autoimmunity 2009
Epidermolysis bullosa acquisita: concise review and practical considerations
JS Lehman, MJ Camilleri, LE Gibson
International Journal of Dermatology 2009
Place of human amniotic membrane immunoblotting in the diagnosis of autoimmune bullous dermatoses
S Grootenboer-Mignot, V Descamps, C Picard-Dahan, P Nicaise-Roland, C Prost-Squarcioni, C Leroux-Villet, C Champagnat, A Delaval, F Aucouturier, B Crickx, S Chollet-Martin
British Journal of Dermatology 2009
Épidermolyse bulleuse acquise de l’enfant
F Bordier-Lamy, C Eschard, M Coste, D Ploton, A Durlach, T Tabary, P Bernard
Annales de Dermatologie et de Vénéréologie 2009

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