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Citations to this article

Elevated glucose impairs endothelium-dependent relaxation by activating protein kinase C.
B Tesfamariam, … , M L Brown, R A Cohen
B Tesfamariam, … , M L Brown, R A Cohen
Published May 1, 1991
Citation Information: J Clin Invest. 1991;87(5):1643-1648. https://doi.org/10.1172/JCI115179.
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Elevated glucose impairs endothelium-dependent relaxation by activating protein kinase C.

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Abstract

A possible relationship between protein kinase C activation and impaired receptor-mediated endothelium-dependent relaxation in diabetes mellitus was examined in isolated aorta from normal rabbit exposed to elevated glucose. Aorta treated for 10 min with 4-phorbol 12-myristate 13-acetate (PMA), a protein kinase C activator, showed decreased relaxations to the endothelium-dependent vasodilator, acetylcholine, similar to normal aorta exposed to elevated glucose (22 and 44 mM) for 6 h. Relaxations to the receptor-independent endothelium-dependent vasodilator, A23187, and those caused by the direct smooth muscle vasodilator, sodium nitroprusside, were unaffected by treatment with PMA or exposure to elevated glucose. Indomethacin increased relaxations to acetylcholine of aorta treated with PMA indicating a role for vasoconstrictor prostanoids. PMA caused a significant increase in basal and acetylcholine-stimulated release of vasoconstrictor prostanoids including thromboxane A2 from aortic segments with, but not without endothelium. Protein kinase C inhibitors, H-7 or sphingosine, restored the abnormal acetylcholine-induced relaxations as well as suppressed the abnormal release of prostanoids in aorta exposed to elevated glucose. These findings suggest that the dysfunction of receptor-mediated endothelium-dependent relaxation associated with exposure to elevated glucose is due to increased production of vasoconstrictor prostanoids by the endothelium as a consequence of protein kinase C activation.

Authors

B Tesfamariam, M L Brown, R A Cohen

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 Total
Citations: 1 1 2 1 1 7 5 4 6 12 5 11 7 15 5 11 10 16 11 12 8 12 19 13 18 12 11 15 13 13 14 12 8 10 321
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Citations to this article in year 2012 (15)

Title and authors Publication Year
Endothelial Dysfunction and Diabetes: Effects on Angiogenesis, Vascular Remodeling, and Wound Healing
GK Kolluru, SC Bir, CG Kevil
International Journal of Vascular Medicine 2012
The Role of Glucosamine-Induced ER Stress in Diabetic Atherogenesis
DR Beriault, GH Werstuck
Experimental Diabetes Research 2012
Vascular effects of intravenous intralipid and dextrose infusions in obese subjects
AR Gosmanov, DD Smiley, L Peng, J Siquiera, G Robalino, C Newton, GE Umpierrez
Metabolism: clinical and experimental 2012
Fibrosis and heart failure
AM Segura, OH Frazier, LM Buja
Heart Failure Reviews 2012
Systemic hemodynamic function in humans with type 1 diabetes treated with protein kinase Cβ inhibition and renin–angiotensin system blockade: a pilot study
DZ Cherney, HN Reich, JW Scholey, V Lai, C Slorach, B Zinman, TJ Bradley
Canadian Journal of Physiology and Pharmacology 2012
Muscle
ML Martin, SL Belmonte, R Ram, FA Kamal, BC Blaxall
Muscle 2012
Cyclophilin-A: a potential screening marker for vascular disease in type-2 diabetes
S Ramachandran, CC Kartha
Canadian Journal of Physiology and Pharmacology 2012
Diabetes and hypertension: the bad companions
E Ferrannini, WC Cushman
The Lancet 2012
Pathophysiology of diabetic erectile dysfunction: potential contribution of vasa nervorum and advanced glycation endproducts
S Cellek, NE Cameron, MA Cotter, A Muneer
International Journal of Impotence Research 2012
Role of oxidative stress in diabetes-mediated vascular dysfunction: Unifying hypothesis of diabetes revisited
SW Schaffer, CJ Jong, M Mozaffari
Vascular Pharmacology 2012
Association of the genetic variants of endothelial nitric oxide synthase gene with angiographically defined coronary artery disease and myocardial infarction in South Indian patients with type 2 diabetes mellitus
P Narne, KC Ponnaluri, S Singh, M Siraj, M Ishaq
Journal of Diabetes and its Complications 2012
The effect of direct renin inhibition alone and in combination with ACE inhibition on endothelial function, arterial stiffness, and renal function in type 1 diabetes
DZ Cherney, JW Scholey, S Jiang, R Har, V Lai, EB Sochett, HN Reich
Diabetes care 2012
Increased Atherothrombotic Burden in Patients with Diabetes Mellitus and Acute Coronary Syndrome: A Review of Antiplatelet Therapy
K Balasubramaniam, GN Viswanathan, SM Marshall, AG Zaman
Cardiology Research and Practice 2012
Perindopril protects against streptozotocin-induced hyperglycemic myocardial damage/alterations
BM Patel, SS Agarwal, SV Bhadada
Human & Experimental Toxicology 2012
Diabetic cardiomyopathy: Pathophysiological mechanisms and cardiac dysfuntion
BR Goyal, AA Mehta
Human & Experimental Toxicology 2012

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Referenced in 12 patents
Referenced in 1 clinical guideline sources
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