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Citations to this article

Lysine and protein metabolism in young women. Subdivision based on the novel use of multiple stable isotopic labels.
C S Irving, … , T W Boutton, P D Klein
C S Irving, … , T W Boutton, P D Klein
Published April 1, 1986
Citation Information: J Clin Invest. 1986;77(4):1321-1331. https://doi.org/10.1172/JCI112437.
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Research Article

Lysine and protein metabolism in young women. Subdivision based on the novel use of multiple stable isotopic labels.

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Abstract

A multitracer stable isotope study of lysine kinetics was carried out in fasted adult female volunteers to determine whether a multicompartmental model that partitions protein synthesis and breakdown into at least two types of tissue components can be constructed from plasma and breath data. Five female subjects, maintained on formula diets, received L-[13C1]lysine (27 mumol/kg) as an i.v. bolus and L-[15N2]lysine (27 mumol/kg) as an oral bolus 4 h postprandially. Plasma and breath samples were collected for 6 h. On an alternate day, subjects received NaH13CO3 (10 mumol/kg) as an i.v. bolus and breath samples were collected for 6 h. Plasma tracer lysine levels were determined by gas chromatography-mass spectrometry isotope ratiometry, and breath 13CO2 levels were measured by mass spectrometric gas isotope ratiometry. The tracer data could be fitted to a mammillary multicompartmental model that consisted of a lysine central compartment and slow- and fast-exchanging peripheral compartments containing 37, 38, and 324 mumol/kg, respectively. The rates of lysine oxidation, incorporation into protein, and release by protein breakdown were 21, 35, and 56 mmol/kg/h, respectively, in the fast-exchanging compartment, whereas the rates of protein synthesis and breakdown in the slow compartment were both 53 mmol/kg/min. These values corresponded to a whole-body lysine flux of 106 mmol/kg/h. The kinetic parameters were in excellent agreement with reported values obtained by constant-infusion methods. The measurements indicated that it will be possible to detect changes in amino acid pool sizes and protein synthesis and breakdown associated with the mobilization of protein stores from plasma and breath measurements in multitracer stable isotope experiments.

Authors

C S Irving, M R Thomas, E W Malphus, L Marks, W W Wong, T W Boutton, P D Klein

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Total citations by year

Year: 2020 2011 2004 2003 1999 1998 1996 1994 1992 1991 1989 1988 1987 1953 Total
Citations: 1 1 1 1 1 1 1 1 2 5 2 1 1 1 20
Citation information
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Citations to this article in year 2004 (1)

Title and authors Publication Year
Transfer of 15 N from oral lactose-ureide to lysine in normal adults
AA Jackson, NR Gibson, R Bundy, A Hounslow, DJ Millward, SA Wootton
International Journal of Food Sciences and Nutrition 2004

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