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Citations to this article

Presence of the Tn antigen on hematopoietic progenitors from patients with the Tn syndrome.
W Vainchenker, … , H Rochant, J P Cartron
W Vainchenker, … , H Rochant, J P Cartron
Published February 1, 1985
Citation Information: J Clin Invest. 1985;75(2):541-546. https://doi.org/10.1172/JCI111730.
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Research Article

Presence of the Tn antigen on hematopoietic progenitors from patients with the Tn syndrome.

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Abstract

The Tn syndrome is an acquired clonal disorder characterized by the exposure of a normally hidden determinant, the Tn antigen, on the surface of human erythrocytes, platelets, granulocytes, and lymphocytes. Two distinct populations, Tn positive (Tn+) and Tn negative (Tn-), of mature hemopoietic cells are present in Tn patients. To determine whether the Tn antigen is already expressed on erythroid, myeloid, and pluripotent progenitors, light-density mononuclear blood cells from two patients with this syndrome were separated by fluorescent-activated cell sorting and by affinity chromatography into Tn+ and Tn- fractions, using their binding properties to Helix pomatia agglutinin (HPA). Burst-forming-unit erythroid (BFU-E), colony-forming-unit granulocyte/macrophage (CFU-GM), cells were assayed in plasma clot cultures. After 12-14 d of culture, colonies were studied by a double fluorescent labeling procedure. First, a fluorescein-conjugated HPA permitted evaluation of the presence or absence of the Tn antigen at the surface of the cells composing each colony, and second, the binding of a murine monoclonal antibody against either glycophorin A (LICR-LON-R10) or against a myeloid antigen (80H5), revealed by an indirect fluorescent procedure, was used to establish the erythroid or myeloid origin of each cell. The Tn+ fraction obtained by cell sorting gave rise to nearly 100% Tn+ colonies composed exclusively of cells bearing this antigen. The reverse was observed for the Tn- cell fraction. These results demonstrate that in the Tn syndrome, BFU-E, CFU-GM, and CFU-GEMM of the Tn+ clone express the Tn antigen at this early stage of differentiation.

Authors

W Vainchenker, G Vinci, U Testa, A Henri, A Tabilio, M P Fache, H Rochant, J P Cartron

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Total citations by year

Year: 2024 2023 2022 2021 2019 2014 2012 2008 2007 2005 2001 2000 1999 1996 1993 1992 1991 1990 1989 Total
Citations: 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 2 1 1 1 21
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Citations to this article (21)

Title and authors Publication Year
Targeting Tn Antigen Suppresses Aberrant O‐Glycosylation‐Elicited Metastasis in Breast Cancer
Du T, Dong X, Tan J, Chen X, Liu J, Wen T, Ru X
Journal of Cellular and Molecular Medicine 2024
Tn antigen interactions of macrophage galactose-type lectin (MGL) in immune function and disease.
Tumoglu B, Keelaghan A, Avci FY
Glycobiology 2023
Emerging Roles of the Unique Molecular Chaperone Cosmc in the Regulation of Health and Disease.
Xiang T, Qiao M, Xie J, Li Z, Xie H
Biomolecules 2022
C1GALT1 in health and disease (Review)
X Sun, M Zhan, X Sun, W Liu, X Meng
Oncology Letters 2021
“Stuck on sugars – how carbohydrates regulate cell adhesion, recognition, and signaling”
RD Cummings
Glycoconjugate Journal 2019
Cosmc is required for T cell persistence in the periphery
CE Cutler, MB Jones, AA Cutler, A Mener, CM Arthur, SR Stowell, RD Cummings
Glycobiology 2019
The Cosmc connection to the Tn antigen in cancer
T Ju, RP Aryal, MR Kudelka, Y Wang, RD Cummings, PA Marino
Cancer biomarkers : section A of Disease markers 2014
Glycosylation of IgA1 and pathogenesis of IgA nephropathy
J Novak, BA Julian, J Mestecky, MB Renfrow
Seminars in Immunopathology 2012
IgA1-secreting cell lines from patients with IgA nephropathy produce aberrantly-glycosylated IgA1
Hitoshi Suzuki, Zina Moldoveanu, Stacy Hall, Rhubell Brown, Huong L. Vu, Lea Novak, Bruce A. Julian, Milan Tomana, Robert J. Wyatt, Jeffrey C. Edberg, Graciella S. Alarcón, Robert P. Kimberly, Yasuhiko Tomino, Jiri Mestecky, Jan Novak
Journal of Clinical Investigation 2008
Comprehensive Glycoscience
S Kelm, R Ravindran
Comprehensive Glycoscience 2007
Mucosal Immunology
WR Brown
Mucosal Immunology 2005
Progress in molecular and genetic studies of IgA nephropathy
J Novak, B A Julian, M Tomana, J Mesteck
Journal of Clinical Immunology 2001
Human Blood Groups
H Schenkel-Brunner
2000
Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies
M Tomana, J Novak, BA Julian, K Matousovic, K Konecny, J Mestecky
Journal of Clinical Investigation 1999
Flow cytometric analysis of T and Tn epitopes on chronic lymphocytic leukemia cells
CT Aller, O Kucuk, GF Springer, A Gilman-Sachs
American Journal of Hematology 1996
Persistent repression of a functional allele can be responsible for galactosyltransferase deficiency in Tn syndrome
M Thurnher, S Rusconi, EG Berger
Journal of Clinical Investigation 1993
Expression of the Tn antigen on erythroid cells from a patient with Tn syndrome
Y Kamitani, E Kajii, T Suda, S Ikemoto
The Japanese Journal of Human Genetics 1992
T cell clones with normal or defective O-galactosylation from a patient with permanent mixed-field polyagglutinability
M Thurnher, EG Berger, H Clausen, W Fierz, A Lanzavecchia
European Journal of Immunology 1992
Aberrant O-linked oligosaccharide biosynthesis in lymphocytes and platelets from patients with the Wiskott-Aldrich syndrome
EA Higgins, KA Siminovitch, DL Zhuang, I Brockhausen, JW Dennis
The Journal of biological chemistry 1991
Flow cytometric analysis of erythrocyte populations in tn syndrome blood using monoclonal antibodies to glycophorin A and the Tn antigen
WL Bigbee, RG Langlois, LH Stanker, M Vanderlaan, RH Jensen
Cytometry 1990
Breast Cancer Immunodiagnosis and Immunotherapy
RL Ceriani
1989

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