Advertisement

Research Article Free access | 10.1172/JCI111730

Presence of the Tn antigen on hematopoietic progenitors from patients with the Tn syndrome.

W Vainchenker, G Vinci, U Testa, A Henri, A Tabilio, M P Fache, H Rochant, and J P Cartron

Find articles by Vainchenker, W. in: JCI | PubMed | Google Scholar

Find articles by Vinci, G. in: JCI | PubMed | Google Scholar

Find articles by Testa, U. in: JCI | PubMed | Google Scholar

Find articles by Henri, A. in: JCI | PubMed | Google Scholar

Find articles by Tabilio, A. in: JCI | PubMed | Google Scholar

Find articles by Fache, M. in: JCI | PubMed | Google Scholar

Find articles by Rochant, H. in: JCI | PubMed | Google Scholar

Find articles by Cartron, J. in: JCI | PubMed | Google Scholar

Published February 1, 1985 - More info

Published in Volume 75, Issue 2 on February 1, 1985
J Clin Invest. 1985;75(2):541–546. https://doi.org/10.1172/JCI111730.
© 1985 The American Society for Clinical Investigation
Published February 1, 1985 - Version history
View PDF
Abstract

The Tn syndrome is an acquired clonal disorder characterized by the exposure of a normally hidden determinant, the Tn antigen, on the surface of human erythrocytes, platelets, granulocytes, and lymphocytes. Two distinct populations, Tn positive (Tn+) and Tn negative (Tn-), of mature hemopoietic cells are present in Tn patients. To determine whether the Tn antigen is already expressed on erythroid, myeloid, and pluripotent progenitors, light-density mononuclear blood cells from two patients with this syndrome were separated by fluorescent-activated cell sorting and by affinity chromatography into Tn+ and Tn- fractions, using their binding properties to Helix pomatia agglutinin (HPA). Burst-forming-unit erythroid (BFU-E), colony-forming-unit granulocyte/macrophage (CFU-GM), cells were assayed in plasma clot cultures. After 12-14 d of culture, colonies were studied by a double fluorescent labeling procedure. First, a fluorescein-conjugated HPA permitted evaluation of the presence or absence of the Tn antigen at the surface of the cells composing each colony, and second, the binding of a murine monoclonal antibody against either glycophorin A (LICR-LON-R10) or against a myeloid antigen (80H5), revealed by an indirect fluorescent procedure, was used to establish the erythroid or myeloid origin of each cell. The Tn+ fraction obtained by cell sorting gave rise to nearly 100% Tn+ colonies composed exclusively of cells bearing this antigen. The reverse was observed for the Tn- cell fraction. These results demonstrate that in the Tn syndrome, BFU-E, CFU-GM, and CFU-GEMM of the Tn+ clone express the Tn antigen at this early stage of differentiation.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

Version history
  • Version 1 (February 1, 1985): No description
Advertisement
Advertisement