Citations to this article
Abstract
The two-color method originally described by Van Rood et al. (Van Rood, J. J., A. Van Leeuwen, and J. S. Ploen. 1976. Simultaneous detection of two cell populations by two-color fluorescence and application to the recognition of B-cell determinants. Nature (Lond.). 262: 795-797) for the typing of homologous leukocytic antibodies, D-region was used for the detection of antilymphocyte antibody (ALA) in systemic lupus erythematosus. In this method, surface immunoglobulin-bearing cells were identified with fluorescein isothiocyanate-labeled anti-immunoglobulin and nuclei of killed cells were stained with ethidium bromide. Therefore, cell type (T or B) of the target cells can be identified without fractionating them. ALA was detected in 87% of lupus sera and had a preferential reactivity with T cells. Its major immunoglobulin class was shown to be immunoglobulin (Ig)M.
Authors
Kunio Okudaira, Hidenori Nakai, Tetsuo Hayakawa, Takamichi Kashiwado, Kiyoaki Tanimoto, Yoshihiko Horiuchi, Takeo Juji
Total citations by year
Citations to this article in year 2014 (1)
| Title and authors | Publication | Year |
|---|---|---|
|
Anti-Lymphocyte Autoantibodies Generate T Cell-C4d Signatures in Systemic Lupus Erythematosus
CC Liu, S Manzi, JM Ahearn |
Translational Research | 2014 |
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