Citations to this article

Androgen Receptor Content of the Normal and Hyperplastic Canine Prostate
Sydney A. Shain, Robert W. Boesel
Sydney A. Shain, Robert W. Boesel
Published March 1, 1978
Citation Information: J Clin Invest. 1978;61(3):654-660. https://doi.org/10.1172/JCI108977.
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Research Article

Androgen Receptor Content of the Normal and Hyperplastic Canine Prostate

Abstract

A procedure was developed for measurement of androgen receptors in cytoplasmic extracts of prostates from intact dogs. The protocol utilized exchange saturation analysis at 15°C employing the synthetic androgen R1881 (17β-hydroxy-17α-methylestra-4,9,11-trien-3-one) as the ligand probe and quantitatively detected total cytoplasmic androgen receptor (Rc, androgen-free receptor, and RcA, androgen-occupied receptor) present at the initiation of the assay. This protocol was employed in conjunction with a tissue mince saturation analysis procedure (for quantitation of nuclear androgen receptor) to quantitate total androgen receptor content of normal and hyperplastic prostates obtained from young (2.5- or 4.6-yr old) and aged (12.5-yr old) purebred dogs of known birth date.

Authors

Sydney A. Shain, Robert W. Boesel

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Year: 2017 2010 2008 1990 1989 1988 1987 1986 1985 1984 1983 1982 1981 1980 1979 Total
Citations: 1 1 1 4 1 2 1 1 1 1 1 6 4 6 4 35
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Citations to this article (35)

Title and authors Publication Year
The impact of finasteride and dutasteride treatments on proliferation, apoptosis, androgen receptor, 5α-reductase 1 and 5α-reductase 2 in TRAMP mouse prostates
AB Opoku-Acheampong, JN Henningson, BL Lindshield 		Heliyon 2017
The Effects of Aging on the Molecular and Cellular Composition of the Prostate Microenvironment
D Bianchi-Frias, F Vakar-Lopez, IM Coleman, SR Plymate, MJ Reed, PS Nelson, ND Leipzig 		PloS one 2010
General Methods and Overviews, Lung Carcinoma and Prostate Carcinoma
MA Hayat 		2008
Steroid Metabolism and Binding in Relation to Prostatic Cell Growth and Differentiation in Vitro
S Chevalier, G Turcotte, G McKercher, P Boulanger, A Chapdelaine 		Annals of the New York Academy of Sciences 1990
Reproductive Pathology of Domestic Mammals
K McEntee 		Reproductive Pathology of Domestic Mammals 1990
Das Prostatakarzinom
G Staehler, PG Fabricius 		1990
Behandlung des fortgeschrittenen Prostatakarzinoms
HG Frohmüller, MP Wirth 		1990
Androgen modulation of prostate cancer cell androgen receptor content is cell line specific
AL Lin, SA Shain 		Molecular and Cellular Endocrinology 1989
Androgen binding as evidenced by a whole cell assay system using cultured canine prostatic epithelial cells
G Turcotte, A Chapdelaine, KD Roberts, S Chevalier 		Journal of Steroid Biochemistry 1988
Multiple binding components for methyltrienolone in canine prostatic epithelial cells
G Turcotte, A Chapdelaine, S Chevalier 		Journal of Steroid Biochemistry 1988
Proliferation of AXC/SSh rat prostate cancer cells in vitro is androgen modulated
SA Shain, RI Huot 		Journal of Steroid Biochemistry 1987
Androgen-Binding Proteins from Human Hyperplastic Prostate as Evaluated by Electrophoresis
E Barahona, H Licea, F Torres, J Sierra, S Ortiz, JA Bermudez 		Systems Biology in Reproductive Medicine 1986
Quantification of cytosolic steroid receptors in secretory and non-secretory epithelial cells of the canine prostate
D Lamarre, S Chevalier, G McKercher, G Bleau, KD Roberts, A Chapdelaine 		Journal of Steroid Biochemistry 1985
Benign Prostatic Hyperplasia: A Disorder of Androgen Metabolism in the Male
R Horton 		Journal of the American Geriatrics Society 1984
Benign Prostatic Hypertrophy
F Hinman, S Boyarsky 		1983
Acute effects of testosterone on serum PGE2 levels in male dogs
LA Klein, JS Stoff, J Ellis 		Prostaglandins 1982
Biochemical Approaches to Aging
M Rothstein 		Biochemical Approaches to Aging 1982
Elevated nuclear androgen receptors in nuclease digests of hyperplastic prostatic tissue of aging dogs
AW Meikle, JD Stringham, JS Wood, GA Taylor 		Biochemical and Biophysical Research Communications 1982
Changes in the mechanisms of hormone and neurotransmitter action during aging: Current status of the role of receptor and post-receptor alterations. A review
GS Roth, GD Hess 		Mechanisms of Ageing and Development 1982
The Endocrinology of Prostate Tumours
R Ghanadian 		1982
Hormones and Cancer
WW Leavitt 		Hormones and Cancer 1982
Prostatic adenocarcinoma with concurrent Sertoli cell tumor in a dog
CW Gill 		The Canadian veterinary journal. La revue vétérinaire canadienne 1981
Elevated intranuclear dihydrotestosterone in prostatic hyperplasia of aging dogs
AW Meikle, ES Collier, JD Stringham, SM Fang, GN Taylor 		Journal of Steroid Biochemistry 1981
Hormone receptors of the baboon cardiovascular system. Biochemical characterization of myocardial cytoplasmic androgen receptors
AL Lin, HC McGill, SA Shain 		Circulation research 1981
Hormone receptors of the baboon cardiovascular system. Biochemical characterization of aortic cytoplasmic androgen receptors
AL Lin, HC McGill, SA Shain 		Arteriosclerosis (Dallas, Tex.) 1981
Androgen- and Estrogen-Receptor Content in Spontaneous and Experimentally Induced Canine Prostatic Hyperplasia
J Trachtenberg, LL Hicks, PC Walsh 		Journal of Clinical Investigation 1980
Endocrine control and physiology of the prostate
AA Sandberg 		The Prostate 1980
The pathogenesis of benign prostatic hyperplasia
JD Wilson 		The American Journal of Medicine 1980
Androgen regulation of androgen receptor content and distribution in the ventral and dorsolateral prostates of aging AXC rats
RW Boesel, RW Klipper, SA Shain 		Steroids 1980
Androgen and Estrogen Receptors in the Canine Prostate
EF Hawkins, J Trachtenberg, LL Hicks, PC Walsh 		Journal of andrology 1980
Prostatic Carcinoma
ES Hafez, E Spring-Mills 		1980
Content of 5α-reduced androgens in subcellular compartments of dog prostate
AW Meikle, GV Burton, JD Stringham, F Sen-Maw 		Journal of Steroid Biochemistry 1979
Binding of androgens in dog prostate cytosol and in plasma
JY Dubé, RR Tremblay, FT Dionne, P Chapdelaine 		Journal of Steroid Biochemistry 1979
Assay of Nuclear Androgen Receptor in Human Prostate
G Lieskovsky, N Bruchovsky 		The Journal of Urology 1979
Testosterone metabolism by the prostate of the aging canine
SA Shain, WM Nitchuk 		Mechanisms of Ageing and Development 1979

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