Kinetics of the Attachment of Intrinsic Factor-Bound Cobamides to Ileal Receptors

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Abstract

To determine whether the molecular configuration of vitamin B12 influences the attachment of intrinsic factor-vitamin B12 complex to ileal microvillous membrane receptor sites, we have examined the kinetics of uptake of intrinsic factor-bound cyanocobalamin by brush borders and microvillous membranes isolated from guinea pig ileum, and have compared this uptake with that of intrinsic factor alone and with that of intrinsic factor complexed with various analogs of cyanocobalamin.

Authors

V. I. Mathan, Bernard M. Babior, Robert M. Donaldson Jr.