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Citations to this article

Studies on collagenase from rheumatoid synovium in tissue culture
John M. Evanson, … , John J. Jeffrey, Stephen M. Krane
John M. Evanson, … , John J. Jeffrey, Stephen M. Krane
Published December 1, 1968
Citation Information: J Clin Invest. 1968;47(12):2639-2651. https://doi.org/10.1172/JCI105947.
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Research Article Article has an altmetric score of 3

Studies on collagenase from rheumatoid synovium in tissue culture

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Abstract

Fragments of synovium from patients with rheumatoid arthritis survive in defined tissue culture medium in the absence of added serum and, after 3-4 days, release into the medium enzyme capable of degrading undenatured collagen. Maximal activity is observed at pH 7-9 but the enzyme is inactive at pH 5. At temperatures of 20° and 27°C, collagen molecules in solution are cleaved into 3/4 and 1/4 length fragments with minimal loss of negative optical rotation, but with loss in specific viscosity of approximately 60%. Above 30°C the fragments begin to denature and denaturation is complete at 37°C. If the enzyme is not inhibited at this stage the large fragments are broken down further to polypeptides of low molecular weight. Reconstituted collagen fibrils and native fibers at 37°C are cleaved to the low molecular weight fragments, although the fibrils are resistant to breakdown at lower temperatures (20°-27°C). It is proposed that the production of such an enzyme by inflamed and proliferating rheumatoid synovium may be responsible for some of the destruction of collagenous structures that accompanies rheumatoid arthritis.

Authors

John M. Evanson, John J. Jeffrey, Stephen M. Krane

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Total citations by year

Year: 2015 2012 2011 2010 2006 2004 2001 2000 1995 1994 1993 1991 1990 1989 1988 1987 1986 1985 1984 1983 1982 1981 1980 1979 1978 1977 1976 1975 1974 1973 1972 1971 1970 1969 1964 Total
Citations: 2 1 3 1 1 1 1 1 2 1 1 1 4 2 3 5 4 4 3 4 7 10 5 5 6 8 7 10 8 9 9 13 7 4 1 154
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Citations to this article in year 2010 (1)

Title and authors Publication Year
Extraction and Purification of Collagenase Enzymes: A Critical Review
Daboor
American Journal of Biochemistry and Biotechnology 2010

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