RNA interference therapeutics targeting angiotensinogen ameliorate preeclamptic phenotype in rodent models
Nadine Haase, … , Babbette LaMarca, Ralf Dechend
Nadine Haase, … , Babbette LaMarca, Ralf Dechend
Published April 27, 2020
Citation Information: J Clin Invest. 2020;130(6):2928-2942. https://doi.org/10.1172/JCI99417.
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Research Article Reproductive biology Therapeutics

RNA interference therapeutics targeting angiotensinogen ameliorate preeclamptic phenotype in rodent models

Abstract

Authors

Nadine Haase, Donald J. Foster, Mark W. Cunningham, Julia Bercher, Tuyen Nguyen, Svetlana Shulga-Morskaya, Stuart Milstein, Sarfraz Shaikh, Jeff Rollins, Michaela Golic, Florian Herse, Kristin Kräker, Ivo Bendix, Meray Serdar, Hanna Napieczynska, Arnd Heuser, Alexandra Gellhaus, Kristin Thiele, Gerd Wallukat, Dominik N. Müller, Babbette LaMarca, Ralf Dechend

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Figure 3

siRNA against hAgt reduces mean arterial pressure and albuminuria.

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siRNA against hAgt reduces mean arterial pressure and albuminuria. (A) E...
(A) Effect of luciferase-targeting siRNA or hAgt-targeting siRNA on mean arterial pressure (MAP) in preeclamptic (PE) rats. MAP measured by telemetry was reduced in hAgt-targeting siRNA–treated (n = 9) compared with luciferase-targeting siRNA–treated (n = 5) PE rats (*P < 0.01, #P < 0.5; 2-way ANOVA with Bonferroni’s post hoc test; mean ± SEM). (B) Effect of luciferase-targeting siRNA or hAgt-targeting siRNA on albuminuria in PE rats. Albuminuria was normalized by hAgt-targeting siRNA treatment (n = 9) compared with luciferase-targeting siRNA treatment (n = 10) (***P < 0.001; unpaired t test; mean ± SEM). (C) Effect of luciferase-targeting siRNA or hAgt-targeting siRNA on Ngal mRNA expression levels in maternal liver and kidney tissue of PE rats. Ngal expression was significantly reduced in maternal kidney of hAgt-targeting versus luciferase-targeting siRNA–treated rats (n = 5 each; *P < 0.05; Mann-Whitney test; mean ± SEM).