Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Embryonic stem cells as sources of donor-independent platelets
Matthew C. Canver, … , Daniel E. Bauer, Stuart H. Orkin
Matthew C. Canver, … , Daniel E. Bauer, Stuart H. Orkin
Published May 11, 2015
Citation Information: J Clin Invest. 2015;125(6):2261-2263. https://doi.org/10.1172/JCI82348.
View: Text | PDF
Commentary

Embryonic stem cells as sources of donor-independent platelets

  • Text
  • PDF
Abstract

The creation of a donor-independent source of platelets has been challenging; however, recent advances show growing promise for alternative platelet sources. Pluripotent stem cells have the capacity to differentiate into mature megakaryocytes with the ability to produce functional platelets. In this issue of JCI, Noh et al. provide a proof-of-principle demonstration that embryonic stem cells can be used to produce platelets on a clinical scale by controlling the level of the transcription factor GATA1. This study emphasizes the importance of precise regulation of gene expression for regenerative medicine applications.

Authors

Matthew C. Canver, Daniel E. Bauer, Stuart H. Orkin

×

Figure 1

Strategy for clinical scale production of platelets ex vivo.

Options: View larger image (or click on image) Download as PowerPoint
Strategy for clinical scale production of platelets ex vivo.
Engineered ...
Engineered ES cells harbor a doxycycline-inducible promoter that mediates the expression of an shRNA that targets Gata1 mRNA, resulting in degradation of the full-length transcript. Addition of doxycycline induces shRNA-mediated GATA1 suppression, leading to logarithmic proliferation of MEP-like cells (G1ME2 cells). The restoration of GATA1 expression upon doxycycline withdrawal supports differentiation of MEP-like cells into mature megakaryocytes capable of producing functional platelets. TRE, tetracylcine response element.

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts