(i) Intradermal/interstitial administration or indirect lymphography results in contrast agent uptake (arrows) into the lymphatic capillary plexus, which is the entry point for the peripheral lymphatics, through collecting and conducting lymphatic vessels, into LNs and eventually into the truncal lymphatics and thoracic duct that drains into the subclavian vein. (ii) Intra-lymphatic administration or direct lymphography consists of cannulating a collecting lymphatic vessel for direct access to the lymphatic vasculature for imaging of peripheral and truncal lymphatics. (iii) Oral gavage of hydrophobic lipids results in uptake through the lacteals into the mesenteric lymphatics that eventually drain to the truncal lymphatics and into the thoracic duct. (iv) Intravenous administration of lymphatic contrast gains access to the lymphatic space predominantly through the high endothelial venules in the LNs or through the reticulo-endothelial system. With the exception of intra-lymphatic administration (ii), these routes of administration are accessible in mouse models of lymphatic disorders.