(A) Alum increases total bone marrow cells in WT but not FANCC-deficient mice. WT, Fancc^+/–, and Fancc^–/– mice were injected with Alum or saline. Bone marrow was harvested 2 weeks later, and total bone marrow mononuclear cells were counted. Statistically significant differences with versus without Alum are indicated by *P < 0.01 or ***P < 0.01 and with versus without FANCC-knockout by **P < 0.01. (B) Alum decreases SCA1⁺ cells and increases CD34⁺ in WT, but not FANCC-deficient, mice. Bone marrow from the mice above was analyzed by flow cytometry for immature (SCA1⁺CD34^–) or committed myeloid progenitors (SCA1^–CD34⁺GR1^–). Statistically significant differences with versus without Alum are indicated by *P < 0.01, **P < 0.01, or ^#P < 0.01 and with versus without FANCC-knockout by ***P < 0.01. (C) Alum increases differentiating granulocytes in the bone marrow of WT mice but not FANCC-deficient mice. The bone marrow above was analyzed for differentiating myeloid progenitors (CD34^–GR1⁺). Statistically significant differences with versus without Alum are indicated by *P < 0.01 and with versus without FANCC-knockout by **P < 0.01. (D) Bone marrow from FANCC-deficient mice is abnormal. Sternal bone marrow from mice above was examined microscopically (original magnification, ×40). Areas of differentiating granulocytes in WT bone marrow after Alum are encircled. A dysplastic megakaryocyte in control Fancc^–/– bone marrow is indicated by an arrow, and hypersegmented granulocytes are indicated by asterisks. Hyaline debris in Fancc^–/– bone marrow from Alum-treated mice is indicated by an arrow, areas of degrading cells are encircled, and abnormal granulocyte progenitors are indicated by asterisks.