Recent data suggest that sex hormones affect the thiazide-sensitive NaCl cotransporter (TSC) density or binding capacity (Chen, Z., D.A. Vaughn, and D.D. Fanestil. 1994. J. Am. Soc. Nephrol. 5:1112-1119). Thus, we determined the effect of ovariectomy (OVX) and estrogen replacement on the ultrastructural localization of TSC in rat kidney using immunocytochemistry. Kidneys of intact female (CON) and OVX rats fed ad libitum for 6 and 9 wk or pair-fed for 9 wk were processed for transmission electron microscopy. Immunogold localization of rat TSC (rTSC1) demonstrated intense label in the apical plasma membrane of CON distal convoluted tubule (DCT). In OVX DCT, rTSC1 label and apical plasma membrane microprojections were decreased. Western blots of renal membrane protein from pair-fed CON and OVX revealed bands at 129-135 kD, but the OVX signal was reduced. Morphometric analyses demonstrated that injecting 10 microg/ kg body weight 17beta-estradiol subcutaneously 4x/wk in OVX rats restored DCT apical microprojections and label density for rTSC1. Thus, in OVX rats (a) rTSC1 immunoreactive renal membrane protein is reduced; (b) apical plasma membrane complexity and immunogold label for rTSC1 in DCT is decreased; and (c) estradiol replacement restores DCT ultrastructure and rTSC1 label to normal. We conclude that estrogen enhances the density of rTSC1 in the DCT, and may alter renal Na transport by this mechanism.
J W Verlander, T M Tran, L Zhang, M R Kaplan, S C Hebert
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Plasma Potassium Negatively Correlates With Sodium Chloride Cotransporter Abundance and Phosphorylation in Urinary Extracellular Vesicles From Patients With Chronic Kidney Disease
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2024 | |
Navigating the multifaceted intricacies of the Na+-Cl− cotransporter, a highly regulated key effector in the control of hydromineral homeostasis
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American Journal of Physiology - Renal Physiology | 2024 |
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American journal of physiology. Renal physiology | 2024 |
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Bioinformatics (Oxford, England) | 2022 |
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Frontiers of Medicine | 2022 |
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2022 | |
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Physiological reviews | 2020 |
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KL Hamilton, DC Devor |
2020 | |
The interplay of renal potassium and sodium handling in blood pressure regulation: critical role of the WNK-SPAK-NCC pathway
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EJ Filippone, M Ruzieh, A Foy |
American journal of kidney diseases : the official journal of the National Kidney Foundation | 2019 |
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E Wolf, EJ Diaz, A Hollis, TA Hoang, HA Azad, KM Bendt, RC Griffiths, MA Sparks |
American journal of physiology. Renal physiology | 2018 |
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American journal of physiology. Renal physiology | 2017 |
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A novel homozygous mutation in the solute carrier family 12 member 3 gene in a Chinese family with Gitelman syndrome
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American journal of physiology. Renal physiology | 2016 |
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A novel SLC12A3 gene homozygous mutation of Gitelman syndrome in an Asian pedigree and literature review
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KL Hamilton, DC Devor |
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Ovarian hormones and prolactin increase renal NaCl cotransporter phosphorylation
L Rojas-Vega, LA Reyes-Castro, V Ramírez, R Bautista-Pérez, C Rafael, M Castañeda-Bueno, P Meade, P los Heros, I Arroyo-Garza, V Bernard, N Binart, NA Bobadilla, J Hadchouel, E Zambrano, G Gamba |
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JA McCormick, DH Ellison |
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The sodium chloride cotransporter SLC12A3: new roles in sodium, potassium, and blood pressure regulation
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MR Berry, C Robinson, FE Frankl |
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PharmGKB summary: diuretics pathway, pharmacodynamics
CF Thorn, DH Ellison, ST Turner, RB Altman, TE Klein |
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CJ Cooper, LD Dworkin, WL Henrich |
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JB Graceli, MA Cicilini, NS Bissoli, GR Abreu, MR Moysés |
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JB Graceli, MA Cicilini, NS Bissoli, GR Abreu, MR Moysés |
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Mechanics and Function of the Pulmonary Vasculature: Implications for Pulmonary Vascular Disease and Right Ventricular Function
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Comprehensive Physiology | 2012 |
Mechanics and Function of the Pulmonary Vasculature: Implications for Pulmonary Vascular Disease and Right Ventricular Function
S Lammers, D Scott, K Hunter, W Tan, R Shandas, KR Stenmark |
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Mechanics and Function of the Pulmonary Vasculature: Implications for Pulmonary Vascular Disease and Right Ventricular Function
S Lammers, D Scott, K Hunter, W Tan, R Shandas, KR Stenmark |
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EI Christensen, CA Wagner, B Kaissling |
Comprehensive Physiology | 2012 |
Brenner and Rector's The Kidney
C Brugnara, KU Eckardt |
Brenner and Rector's The Kidney | 2012 |
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Climacteric | 2012 |
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EM Rodenburg, BH Stricker, LE Visser |
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Cellular and Molecular Life Sciences | 2011 |
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NV Ocampo, J Tafreshi, CL Hauschild, RG Pai |
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Hypokalemic Thyrotoxic Periodic Paralysis With Thyrotoxic Psychosis and Hypercapnic Respiratory Failure
B Abbasi, Z Sharif, LR Sprabery |
The American Journal of the Medical Sciences | 2010 |
Pulmonary surfactant: an immunological perspective
ZC Chroneos, Z Sever-Chroneos, VL Shepherd |
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | 2009 |
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V Vallon, J Schroth, F Lang, D Kuhl, S Uchida |
American journal of physiology. Renal physiology | 2009 |
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Nephrology | 2009 |
Expression and phosphorylation of the Na + -Cl − cotransporter NCC in vivo is regulated by dietary salt, potassium, and SGK1
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American journal of physiology. Renal physiology | 2009 |
Renal Cell Cancer
JJ de la Rosette, CN Sternberg, HP van Poppel |
2008 | |
Gitelman’s syndrome: towards genotype-phenotype correlations?
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Pediatric Nephrology | 2007 |
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C Esteva-Font, RT Balcells, P Fernández-Llama |
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F Li, CE Wood, M Keller-Wood |
American Journal of Physiology - Regulatory, Integrative and Comparative Physiology | 2007 |
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W Zheng, JW Verlander, IJ Lynch, M Cash, J Shao, LR Stow, BD Cain, ID Weiner, SM Wall, CS Wingo |
American journal of physiology. Renal physiology | 2007 |
ANG II provokes acute trafficking of distal tubule Na + -Cl − cotransporter to apical membrane
MB Sandberg, AD Riquier, K Pihakaski-Maunsbach, AA McDonough, AB Maunsbach |
American journal of physiology. Renal physiology | 2007 |
17β-Estradiol deficiency reduces potassium excretion in an angiotensin type 1 receptor-dependent manner
H Ji, W Zheng, C Falconetti, DM Roesch, SE Mulroney, K Sandberg |
American journal of physiology. Heart and circulatory physiology | 2007 |
The thiazide-sensitive NaCl cotransporter: a new target for acute regulation of salt and water transport by angiotensin II
JW Verlander |
American journal of physiology. Renal physiology | 2007 |
Sodium transporters in the distal nephron and disease implications
CA Ecelbarger, S Tiwari |
Current Hypertension Reports | 2006 |
17-β Estradiol attenuates streptozotocin-induced diabetes and regulates the expression of renal sodium transporters
S Riazi, C Maric, CA Ecelbarger |
Kidney International | 2006 |
Gitelman’s syndrome: towards genotype-phenotype correlations?
E Riveira-Munoz, Q Chang, RJ Bindels, O Devuyst |
Pediatric Nephrology | 2006 |
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Estrogens contribute to a sex difference in plasma potassium concentration: A mechanism for regulation of adrenal angiotensin receptors
W Zheng, M Shi, SE You, H Ji, DM Roesch |
Gender Medicine | 2006 |
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Gender Medicine | 2006 |
Redistribution of distal tubule Na + -Cl − cotransporter (NCC) in response to a high-salt diet
MB Sandberg, AB Maunsbach, AA McDonough |
American journal of physiology. Renal physiology | 2006 |
Risk factors for renal cell carcinoma: Hypertension or diuretics?
FH Messerli |
Kidney International | 2005 |
Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters
G Gamba |
Physiological reviews | 2005 |
Intercalated cell H + /OH − transporter expression is reduced in Slc26a4 null mice
YH Kim, JW Verlander, SW Matthews, I Kurtz, W Shin, ID Weiner, LA Everett, ED Green, S Nielsen, SM Wall |
American journal of physiology. Renal physiology | 2005 |
Intrafamilial phenotype variability in patients with Gitelman syndrome having the same mutations in their thiazide-sensitive sodium/chloride cotransporter
SH Lin, NL Cheng, YJ Hsu, ML Halperin |
American Journal of Kidney Diseases | 2004 |
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GH Kim |
American Journal of Nephrology | 2004 |
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KM Chow, BC Kwan, CC Szeto |
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NS Stachenfeld, HS Taylor, CA Leone, DL Keefe |
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C Li, W Wang, TH Kwon, MA Knepper, S Nielsen, J Frøkiær |
American journal of physiology. Renal physiology | 2003 |
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J Loffing, B Kaissling |
American journal of physiology. Renal physiology | 2003 |
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Journal of the American Society of Nephrology : JASN | 2003 |
Human Cortical Distal Nephron: Distribution of Electrolyte and Water Transport Pathways
HL Biner, MP Arpin-Bott, J Loffing, X Wang, M Knepper, SC Hebert, B Kaissling |
Journal of the American Society of Nephrology : JASN | 2002 |
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MA Knepper, HL Brooks |
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DN Cruz, AJ Shaer, MJ Bia, RP Lifton, DB Simon |
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Antihypertensive Therapy and Cancer Risk:
DC Felmeden, GY Lip |
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Antihypertensive therapy: beta-blockers and diuretics-why do physicians not always follow guidelines?
FH Messerli |
Proceedings (Baylor University. Medical Center) | 2000 |
Antihypertensive Therapy: Beta-Blockers and Diuretics—Why Do Physicians Not Always Follow Guidelines?
FH Messerli |
Proceedings (Baylor University. Medical Center) | 2000 |
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RF Reilly, DH Ellison |
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Molecular biology of distal nephron sodium transport mechanisms
G Gamba |
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S Bachmann |
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E Grossman, FH Messerli |
Kidney International | 1999 |
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