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Citations to this article

New protective antigen of group A streptococci
James B. Dale, … , Harry S. Courtney, David L. Hasty
James B. Dale, … , Harry S. Courtney, David L. Hasty
Published May 1, 1999
Citation Information: J Clin Invest. 1999;103(9):1261-1268. https://doi.org/10.1172/JCI5118.
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New protective antigen of group A streptococci

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Abstract

It is widely believed that the surface M protein of group A streptococci is the predominant surface protein of these organisms containing opsonic epitopes. In the present study, we identified a new surface protein, distinct from M protein, that evokes protective antibodies. A type 18 M-negative mutant was found to be both resistant to phagocytosis in human blood and virulent in mice. The wild-type strain, but not the M-negative mutant, was opsonized by antisera against purified recombinant M18 protein or a synthetic peptide copying the NH2-terminus of M18. However, antisera raised against a crude pepsin extract of the M-negative mutant opsonized both strains, indicating the presence of a protective antigen in addition to type 18 M protein. This antiserum was used to identify and purify a 24-kDa protein fragment (Spa, streptococcal protective antigen) that evoked antibodies that opsonized the M18 parent and the M-negative mutant. The results of passive mouse protection tests confirmed the presence of protective epitopes within Spa. The deduced amino acid sequence of a 636-bp 5′ fragment of the spa18 gene showed no homology with sequences in GenBank. These studies reveal the presence of a new protective antigen of certain strains of group A streptococci that may prove to be an important component of vaccines to prevent streptococcal infections.

Authors

James B. Dale, Edna Y. Chiang, Shaoyou Liu, Harry S. Courtney, David L. Hasty

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Total citations by year

Year: 2020 2019 2018 2017 2016 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 Total
Citations: 1 1 1 2 1 2 2 8 1 2 5 5 1 4 3 3 6 6 8 2 64
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