Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Spatiotemporal trafficking of HIV in human plasmacytoid dendritic cells defines a persistently IFN-α–producing and partially matured phenotype
Meagan O’Brien, … , David Levy, Nina Bhardwaj
Meagan O’Brien, … , David Levy, Nina Bhardwaj
Published February 21, 2011
Citation Information: J Clin Invest. 2011;121(3):1088-1101. https://doi.org/10.1172/JCI44960.
View: Text | PDF
Research Article Virology

Spatiotemporal trafficking of HIV in human plasmacytoid dendritic cells defines a persistently IFN-α–producing and partially matured phenotype

  • Text
  • PDF
Abstract

Plasmacytoid DCs (pDCs) are innate immune cells that are specialized to produce IFN-α and to activate adaptive immune responses. Although IFN-α inhibits HIV-1 replication in vitro, the production of IFN-α by HIV-activated pDCs in vivo may contribute more to HIV pathogenesis than to protection. We have now shown that HIV-stimulated human pDCs allow for persistent IFN-α production upon repeated stimulation, express low levels of maturation molecules, and stimulate weak T cell responses. Persistent IFN-α production by HIV-stimulated pDCs correlated with increased levels of IRF7 and was dependent upon the autocrine IFN-α/β receptor feedback loop. Because it has been shown that early endosomal trafficking of TLR9 agonists causes strong activation of the IFN-α pathway but weak activation of the NF-κB pathway, we sought to investigate whether early endosomal trafficking of HIV, a TLR7 agonist, leads to the IFN-α–producing phenotype we observed. We demonstrated that HIV preferentially traffics to the early endosome in human pDCs and therefore skews pDCs toward a partially matured, persistently IFN-α–secreting phenotype.

Authors

Meagan O’Brien, Olivier Manches, Rachel Lubong Sabado, Sonia Jimenez Baranda, Yaming Wang, Isabelle Marie, Linda Rolnitzky, Martin Markowitz, David M. Margolis, David Levy, Nina Bhardwaj

×

Figure 1

HIV-activated pDCs are not refractory to restimulation to produce IFN-α.

Options: View larger image (or click on image) Download as PowerPoint
HIV-activated pDCs are not refractory to restimulation to produce IFN-α....
(A and B) pDCs were incubated with media alone (0), AT-2 HIV (AT2), live HIV (LMN), R848, influenza virus (Flu), Sendai virus, CpGA, or CpGB for 18 hours. Supernatants were removed, and cells were washed, counted, and restimulated for 18 hours (restimulation denoted as 0-0, AT2-AT2, LMN-LMN, etc.). IFN-α was (A) measured in the supernatants by ELISA and (B) corrected for cell number. (C) Experiments were repeated for 6–10 donors. Percent IFN-α restimulation denotes IFN-α produced after the second stimulation relative to that after the first. Dots represent mean of 2 replicates per donor. (D) HIV-stimulated pDCs could be restimulated with different TLR ligands, whereas R848-stimulated pDCs could not be restimulated. Data are means (n = 2 replicates) and representative of 2 independent experiments. (E) pDCs were stimulated with media or AT-2 HIV for 12 hours; sorted; separated into IFN-α–secreting and non–IFN-α–secreting cells; and then stimulated with AT-2 HIV for 24 hours. AT-2 HIV–activated IFN-α–secreting pDCs produced more cytokine per cell than did HIV-activated pDCs that did not produce IFN-α upon initial stimulation. Data are means (n = 2 replicates) and representative of 2 independent experiments. (F) Culture supernatants were applied to a COS1 pRLpISRE cell line that produces luciferase upon exposure to bioactive type I IFNs. pDCs produced bioactive type I IFN upon repeated stimulation with HIV. Data are mean ± SEM (n = 2 replicates) and representative of 3 independent experiments.

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts