Plastic adherence of FN peptides. Effect of the coating concentration on the
absorption of recombinant FN fragments to nontissue culture-treated plastic
(A) and on the subsequent binding of mouse BM cells or human
HEL cells (B). Bound FN was detected by ELISA (average of 4
wells/concentration) using antibody FN12-8 (A), which is specific
for the CELL-binding domain of FN. (A) Fragment H-271 (filled
squares), C-274 (open squares), CH-271(filled circles), H-296 (open circles),
and CH-296 (filled triangles). For clarity, error bars have been omitted.
(B) Binding of human HEL cells (squares) or low-density mouse
BM cells (circles) to plates coated with H-271 (open symbols) or CH-296 (closed
symbols).