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Citations to this article

Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39
Matthew C. Hyman, … , Aaron J. Marcus, David J. Pinsky
Matthew C. Hyman, … , Aaron J. Marcus, David J. Pinsky
Published April 20, 2009
Citation Information: J Clin Invest. 2009;119(5):1136-1149. https://doi.org/10.1172/JCI36433.
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Research Article Cardiology Article has an altmetric score of 3

Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39

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Abstract

Leukocyte and platelet accumulation at sites of cerebral ischemia exacerbate cerebral damage. The ectoenzyme CD39 on the plasmalemma of endothelial cells metabolizes ADP to suppress platelet accumulation in the ischemic brain. However, the role of leukocyte surface CD39 in regulating monocyte and neutrophil trafficking in this setting is not known. Here we have demonstrated in mice what we believe to be a novel mechanism by which CD39 on monocytes and neutrophils regulates their own sequestration into ischemic cerebral tissue, by catabolizing nucleotides released by injured cells, thereby inhibiting their chemotaxis, adhesion, and transmigration. Bone marrow reconstitution and provision of an apyrase, an enzyme that hydrolyzes nucleoside tri- and diphosphates, each normalized ischemic leukosequestration and cerebral infarction in CD39-deficient mice. Leukocytes purified from Cd39–/– mice had a markedly diminished capacity to phosphohydrolyze adenine nucleotides and regulate platelet reactivity, suggesting that leukocyte ectoapyrases modulate the ambient vascular nucleotide milieu. Dissipation of ATP by CD39 reduced P2X7 receptor stimulation and thereby suppressed baseline leukocyte αMβ2-integrin expression. As αMβ2-integrin blockade reversed the postischemic, inflammatory phenotype of Cd39–/– mice, these data suggest that phosphohydrolytic activity on the leukocyte surface suppresses cell-cell interactions that would otherwise promote thrombosis or inflammation. These studies indicate that CD39 on both endothelial cells and leukocytes reduces inflammatory cell trafficking and platelet reactivity, with a consequent reduction in tissue injury following cerebral ischemic challenge.

Authors

Matthew C. Hyman, Danica Petrovic-Djergovic, Scott H. Visovatti, Hui Liao, Sunitha Yanamadala, Diane Bouïs, Enming J. Su, Daniel A. Lawrence, M. Johan Broekman, Aaron J. Marcus, David J. Pinsky

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Total citations by year

Year: 2024 2023 2022 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 Total
Citations: 2 3 2 1 4 2 5 6 5 9 1 6 11 2 1 60
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2011 (11)

Title and authors Publication Year
The immunology of stroke: from mechanisms to translation
C Iadecola, J Anrather
Nature Medicine 2011
Immune cell regulation by autocrine purinergic signalling
WG Junger
Nature Reviews Immunology 2011
Liver damage and systemic inflammatory responses are exacerbated by the genetic deletion of CD39 in total hepatic ischemia
X Sun, M Imai, M Nowak-Machen, O Guckelberger, K Enjyoji, Y Wu, Z Khalpey, P Berberat, J Munasinghe, SC Robson
Purinergic Signalling 2011
Emerging role of extracellular nucleotides and adenosine in multiple sclerosis
M Cieślak, F Kukulski, M Komoszyński
Purinergic Signalling 2011
Transgenic over expression of ectonucleotide triphosphate diphosphohydrolase-1 protects against murine myocardial ischemic injury
M Cai, ZM Huttinger, H He, W Zhang, F Li, LA Goodman, DG Wheeler, LJ Druhan, JL Zweier, KM Dwyer, G He, AJ d'Apice, SC Robson, PJ Cowan, RJ Gumina
Journal of Molecular and Cellular Cardiology 2011
Cellular and molecular choreography of neutrophil recruitment to sites of sterile inflammation
B McDonald, P Kubes
Journal of Molecular Medicine 2011
Regulation of innate immunity by the nucleotide pathway in children with idiopathic nephrotic syndrome
R Bertelli, M Bodria, M Nobile, S Alloisio, R Barbieri, G Montobbio, P Patrone, GM Ghiggeri
Clinical & Experimental Immunology 2011
The scavenger receptor CD36 contributes to the neurotoxicity of bone marrow-derived monocytes through peroxynitrite production
P Zhou, L Qian, EF Gallo, RS Deeb, J Anrather, SS Gross, C Iadecola
Neurobiology of Disease 2011
CD39 Expression on T Lymphocytes Correlates With Severity of Disease in Patients With Chronic Lymphocytic Leukemia
D Pulte, RR Furman, MJ Broekman, JH Drosopoulos, HS Ballard, KE Olson, JR Kizer, AJ Marcus
Clinical Lymphoma Myeloma and Leukemia 2011
Lipid metabolism modulation by the P2X7 receptor in the immune system and during the course of infection: new insights into the old view
HM Costa-Junior, C Marques-da-Silva, FS Vieira, LC Monção-Ribeiro, R Coutinho-Silva
Purinergic Signalling 2011
The neutrophil in vascular inflammation
M Phillipson, P Kubes
Nature Medicine 2011

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