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Citations to this article

Fluorescent pegylated nanoparticles demonstrate fluid-phase pinocytosis by macrophages in mouse atherosclerotic lesions
Chiara Buono, … , Marcelo Amar, Howard S. Kruth
Chiara Buono, … , Marcelo Amar, Howard S. Kruth
Published April 13, 2009
Citation Information: J Clin Invest. 2009;119(5):1373-1381. https://doi.org/10.1172/JCI35548.
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Technical Advance Cardiology Article has an altmetric score of 3

Fluorescent pegylated nanoparticles demonstrate fluid-phase pinocytosis by macrophages in mouse atherosclerotic lesions

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Abstract

The uptake of lipoproteins by macrophages is a critical step in the development of atherosclerotic lesions. Cultured monocyte-derived macrophages take up large amounts of native LDL by receptor-independent fluid-phase pinocytosis, either constitutively or in response to specific activating stimuli, depending on the macrophage phenotype. We therefore sought to determine whether fluid-phase pinocytosis occurs in vivo in macrophages in atherosclerotic lesions. We demonstrated that fluorescent pegylated nanoparticles similar in size to LDL (specifically nontargeted Qtracker quantum dot and AngioSPARK nanoparticles) can serve as models of LDL uptake by fluid-phase pinocytosis in cultured human monocyte–derived macrophages and mouse bone marrow–derived macrophages. Using fluorescence microscopy, we showed that atherosclerosis-prone Apoe-knockout mice injected with these nanoparticles displayed massive accumulation of the nanoparticles within CD68+ macrophages, including lipid-containing foam cells, in atherosclerotic lesions in the aortic arch. Similar results were obtained when atherosclerotic mouse aortas were cultured with nanoparticles in vitro. These results show that macrophages within atherosclerotic lesions can take up LDL-sized nanoparticles by fluid-phase pinocytosis and indicate that fluid-phase pinocytosis of LDL is a mechanism for macrophage foam cell formation in vivo.

Authors

Chiara Buono, Joshua J. Anzinger, Marcelo Amar, Howard S. Kruth

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2013 2012 2011 2010 2009 Total
Citations: 1 1 1 4 2 1 1 3 7 2 1 5 4 6 7 1 47
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2013 (5)

Title and authors Publication Year
Fluid-Phase Pinocytosis of Native Low Density Lipoprotein Promotes Murine M-CSF Differentiated Macrophage Foam Cell Formation
MK Barthwal, JJ Anzinger, Q Xu, T Bohnacker, MP Wymann, HS Kruth
PloS one 2013
Fluid-Phase Pinocytosis of LDL by Macrophages: A Novel Target to Reduce Macrophage Cholesterol Accumulation in Atherosclerotic Lesions
H Kruth
Current pharmaceutical design 2013
Labeling Cytosolic Targets in Live Cells with Blinking Probes
J Xu, J Chang, Q Yan, T Dertinger, MP Bruchez, S Weiss
The Journal of Physical Chemistry Letters 2013
Peripherally administered nanoparticles target monocytic myeloid cells, secondary lymphoid organs and tumors in mice
IC Kourtis, S Hirosue, A Titta, S Kontos, T Stegmann, JA Hubbell, MA Swartz
PloS one 2013
Differential regulation of macropinocytosis in macrophages by cytokines: Implications for foam cell formation and atherosclerosis☆
DR Michael, TG Ashlin, CS Davies, H Gallagher, TW Stoneman, ML Buckley, DP Ramji
Cytokine 2013

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Referenced in 12 patents
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