Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Pancreatic Cancer (Jul 2025)
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
The FGF system has a key role in regulating vascular integrity
Masahiro Murakami, … , Radu V. Stan, Michael Simons
Masahiro Murakami, … , Radu V. Stan, Michael Simons
Published September 5, 2008
Citation Information: J Clin Invest. 2008;118(10):3355-3366. https://doi.org/10.1172/JCI35298.
View: Text | PDF | Corrigendum
Research Article Vascular biology Article has an altmetric score of 3

The FGF system has a key role in regulating vascular integrity

  • Text
  • PDF
Abstract

The integrity of the endothelial monolayer is essential to blood vessel homeostasis and active regulation of endothelial permeability. The FGF system plays important roles in a wide variety of physiologic and pathologic conditions; however, its role in the adult vasculature has not been defined. To assess the role of the FGF system in the adult endothelial monolayer, we disrupted FGF signaling in bovine aortic endothelial cells and human saphenous vein endothelial cells in vitro and in adult mouse and rat endothelial cells in vivo using soluble FGF traps or a dominant inhibitor of all FGF receptors. The inhibition of FGF signaling using these approaches resulted in dissociation of the VE-cadherin/p120-catenin complex and disassembly of adherens and tight junctions, which progressed to loss of endothelial cells, severe impairment of the endothelial barrier function, and finally, disintegration of the vasculature. Thus, FGF signaling plays a key role in the maintenance of vascular integrity.

Authors

Masahiro Murakami, Loc T. Nguyen, Zhen W. Zhang, Karen L. Moodie, Peter Carmeliet, Radu V. Stan, Michael Simons

×

Figure 5

Increased endothelial permeability in cells lacking FGF signaling.

Options: View larger image (or click on image) Download as PowerPoint
Increased endothelial permeability in cells lacking FGF signaling.
(A) E...
(A) Endothelial monolayer permeability evaluated with ECIS system. Impedance was measured every 5 minutes for 48 hours after the onset of adenoviral transduction. After adenoviral exposure, cell culture medium was changed to normal growth medium. n = 3 in each group, P < 0.05, control versus FGFR1DN or sFGFR1IIIc at 48 hours (B) ESIS analysis using various sFGFR adenoviruses. After adenoviral exposure, medium was replaced with normal growth medium. n = 3 in each group, P < 0.05, control versus sFGFR1IIIc or sFGFR3IIIc at 48 hours (C) Transwell tracer experiment using high (2 MDa) and low (70 kDa) molecular weight dextran. Full confluent BAECs on the Transwell chambers were transduced and dextrans were added in the upper chamber. Fluorescent values in the lower chamber were measured at indicated time points using a fluorescent microplate reader. Three independent experiments were performed. One representative experiment is shown as mean ± SD for n = 3 in each group; *P < 0.05 by Student’s t test, control vs. FGFR1DN. AU represents relative intensity of fluorescence. (D) Transwell tracer experiment using Ad-sFGFRs. Data are shown as mean ± SD for n = 3 in each group; *P < 0.05 by Student’s t test, control vs. sFGFR1IIIc.

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts

Referenced in 6 patents
174 readers on Mendeley
1 readers on CiteULike
See more details