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Citations to this article

Production of CFTR-null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus–mediated gene targeting and somatic cell nuclear transfer
Christopher S. Rogers, … , Michael J. Welsh, Randall S. Prather
Christopher S. Rogers, … , Michael J. Welsh, Randall S. Prather
Published March 6, 2008
Citation Information: J Clin Invest. 2008;118(4):1571-1577. https://doi.org/10.1172/JCI34773.
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Technical Advance Article has an altmetric score of 13

Production of CFTR-null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus–mediated gene targeting and somatic cell nuclear transfer

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Abstract

Progress toward understanding the pathogenesis of cystic fibrosis (CF) and developing effective therapies has been hampered by lack of a relevant animal model. CF mice fail to develop the lung and pancreatic disease that cause most of the morbidity and mortality in patients with CF. Pigs may be better animals than mice in which to model human genetic diseases because their anatomy, biochemistry, physiology, size, and genetics are more similar to those of humans. However, to date, gene-targeted mammalian models of human genetic disease have not been reported for any species other than mice. Here we describe the first steps toward the generation of a pig model of CF. We used recombinant adeno-associated virus (rAAV) vectors to deliver genetic constructs targeting the CF transmembrane conductance receptor (CFTR) gene to pig fetal fibroblasts. We generated cells with the CFTR gene either disrupted or containing the most common CF-associated mutation (ΔF508). These cells were used as nuclear donors for somatic cell nuclear transfer to porcine oocytes. We thereby generated heterozygote male piglets with each mutation. These pigs should be of value in producing new models of CF. In addition, because gene-modified mice often fail to replicate human diseases, this approach could be used to generate models of other human genetic diseases in species other than mice.

Authors

Christopher S. Rogers, Yanhong Hao, Tatiana Rokhlina, Melissa Samuel, David A. Stoltz, Yuhong Li, Elena Petroff, Daniel W. Vermeer, Amanda C. Kabel, Ziying Yan, Lee Spate, David Wax, Clifton N. Murphy, August Rieke, Kristin Whitworth, Michael L. Linville, Scott W. Korte, John F. Engelhardt, Michael J. Welsh, Randall S. Prather

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PloS one 2014
Intestinal CFTR Expression Alleviates Meconium Ileus in Cystic Fibrosis Pigs
David Stoltz, Tatiana Rokhlina, Sarah Ernst, Alejandro Pezzulo, Lynda Ostedgaard, Philip Karp, Melissa Samuels, Leah Reznikov, Michael Rector, Nicholas Gansemer, Drake Bouzek, Mahmoud Abou Alaiwa, Mark Hoegger, Paula Ludwig, Peter Taft, Tanner Wallen, Christine Wohlford-Lenane, James McMenimen, Jeng-Haur Chen, Katrina Bogan, Ryan Adam, Emma Hornick, George Nelson, Eric Hoffman, Eugene Chang, Joseph Zabner, Paul B. McCray, Jr., Randall Prather, David K. Meyerholz, Michael Welsh
Journal of Clinical Investigation 2013
Intrapulmonary Versus Nasal Transduction of Murine Airways With GP64-pseudotyped Viral Vectors
M Oakland, W Maury, PB McCray, PL Sinn
Molecular Therapy — Nucleic Acids 2013
Gene delivery to the airway
NW Keiser, JF Engelhardt
Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.] 2013
A Lung Segmental Model of Chronic Pseudomonas Infection in Sheep
D Collie, J Govan, S Wright, E Thornton, P Tennant, S Smith, C Doherty, G McLachlan, E Gulbins
PloS one 2013
Genetically Engineered Pig Models for Human Diseases
RS Prather, M Lorson, JW Ross, JJ Whyte, E Walters
Annual Review of Animal Biosciences 2013
Adenoviral Gene Transfer Corrects the Ion Transport Defect in the Sinus Epithelia of a Porcine CF Model
AE Potash, TJ Wallen, PH Karp, S Ernst, TO Moninger, ND Gansemer, DA Stoltz, J Zabner, EH Chang
Molecular Therapy 2013
Airway Deposition of Nebulized Gene Delivery Nanocomplexes Monitored by Radioimaging Agents
MD Manunta, RJ McAnulty, A McDowell, J Jin, D Ridout, J Fleming, SE Bottoms, L Tossici-Bolt, GJ Laurent, L Biassoni, C O’Callaghan, SL Hart
American journal of respiratory cell and molecular biology 2013
Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set
M Kurome, L Geistlinger, B Kessler, V Zakhartchenko, N Klymiuk, A Wuensch, A Richter, A Baehr, K Kraehe, K Burkhardt, K Flisikowski, T Flisikowska, C Merkl, M Landmann, M Durkovic, A Tschukes, S Kraner, D Schindelhauer, T Petri, A Kind, H Nagashima, A Schnieke, R Zimmer, E Wolf
BMC Biotechnology 2013
Air Trapping and Airflow Obstruction in Newborn Cystic Fibrosis Piglets
RJ Adam, AS Michalski, C Bauer, MH Alaiwa, TJ Gross, MS Awadalla, DC Bouzek, ND Gansemer, PJ Taft, MJ Hoegger, A Diwakar, M Ochs, JM Reinhardt, EA Hoffman, RR Beichel, DK Meyerholz, DA Stoltz
American journal of respiratory and critical care medicine 2013
Genetically modified pigs to model human diseases
T Flisikowska, A Kind, A Schnieke
Journal of Applied Genetics 2013
Natural genotypes via genetic engineering
KD Wells
Proceedings of the National Academy of Sciences 2013
Early Airway Structural Changes in Cystic Fibrosis Pigs as a Determinant of Particle Distribution and Deposition
M Awadalla, S Miyawaki, MH Alaiwa, RJ Adam, DC Bouzek, AS Michalski, MK Fuld, KJ Reynolds, EA Hoffman, CL Lin, DA Stoltz
Annals of Biomedical Engineering 2013
Immunization of aged pigs with attenuated pseudorabies virus vaccine combined with CpG oligodeoxynucleotide restores defective Th1 immune responses
F Ming, J Yang, P Chu, M Ma, J Shi, H Cai, C Huang, H Li, Z Jiang, H Wang, W Wang, S Zhang, L Zhang
PloS one 2013
Production of cloned pigs with targeted attenuation of gene expression
V Bordignon, N El-Beirouthi, BG Gasperin, MS Albornoz, MA Martinez-Diaz, C Schneider, D Laurin, D Zadworny, LB Agellon
PloS one 2013
CFTR-deficient pigs display peripheral nervous system defects at birth
LR Reznikov, Q Dong, JH Chen, TO Moninger, JM Park, Y Zhang, J Du, MS Hildebrand, RJ Smith, CO Randak, DA Stoltz, MJ Welsh
Proceedings of the National Academy of Sciences 2013
The Cystic Fibrosis Gene: A Molecular Genetic Perspective
LC Tsui, R Dorfman
Cold Spring Harbor Perspectives in Medicine 2013
From in vitro to in vivo Models of Bacterial Biofilm-Related Infections
D Lebeaux, A Chauhan, O Rendueles, C Beloin
Pathogens 2013
Sinus hypoplasia precedes sinus infection in a porcine model of cystic fibrosis
EH Chang, AA Pezzulo, DK Meyerholz, AE Potash, TJ Wallen, LR Reznikov, JC Sieren, PH Karp, S Ernst, TO Moninger, ND Gansemer, PB McCray, DA Stoltz, MJ Welsh, J Zabner
The Laryngoscope 2012
Cystic fibrosis: a mucosal immunodeficiency syndrome
TS Cohen, A Prince
Nature Medicine 2012
Advances in Cell and Gene-based Therapies for Cystic Fibrosis Lung Disease
M Oakland, PL Sinn, PB McCray
Molecular Therapy 2012
Why mouse airway submucosal gland serous cells do not secrete fluid in response to cAMP stimulation
RJ Lee, JK Foskett
The Journal of biological chemistry 2012
Generation of SV40-transformed rabbit tracheal-epithelial-cell-derived blastocyst by somatic cell nuclear transfer
D Semir, R Maurisse, F Du, J Xu, X Yang, B Illek, DC Gruenert
Cell & Tissue Research 2012
Requirements for ion and solute transport, and pH regulation during enamel maturation
RS Lacruz, CE Smith, P Moffatt, EH Chang, TG Bromage, P Bringas, A Nanci, SK Baniwal, J Zabner, MJ Welsh, I Kurtz, ML Paine
Journal of Cellular Physiology 2012
Genetically modified pigs for biomedical research
Y Luo, L Lin, L Bolund, TG Jensen, CB Sørensen
Journal of Inherited Metabolic Disease 2012
Reduced airway surface pH impairs bacterial killing in the porcine cystic fibrosis lung
AA Pezzulo, XX Tang, MJ Hoegger, MH Alaiwa, S Ramachandran, TO Moninger, PH Karp, CL Wohlford-Lenane, HP Haagsman, M Eijk, B Bánfi, AR Horswill, DA Stoltz, PB McCray, MJ Welsh, J Zabner
Nature 2012
Computer-aided analysis of airway trees in micro-CT scans of ex vivo porcine lung tissue
C Bauer, R Adam, DA Stoltz, RR Beichel
Computerized Medical Imaging and Graphics 2012
Generation of minipigs with targeted transgene insertion by recombinase-mediated cassette exchange (RMCE) and somatic cell nuclear transfer (SCNT)
JE Jakobsen, MG Johansen, M Schmidt, F Dagnæs-Hansen, K Dam, A Gunnarsson, Y Liu, PM Kragh, R Li, IE Holm, H Callesen, JG Mikkelsen, AL Nielsen, AL Jørgensen
Transgenic Research 2012
Completion of the swine genome will simplify the production of swine as a large animal biomedical model
EM Walters, E Wolf, JJ Whyte, J Mao, S Renner, H Nagashima, E Kobayashi, J Zhao, KD Wells, JK Critser, LK Riley, RS Prather
BMC Medical Genomics 2012
Lentiviral vector gene transfer to porcine airways
PL Sinn, AL Cooney, M Oakland, DE Dylla, TJ Wallen, AA Pezzulo, EH Chang, PB McCray
Molecular Therapy — Nucleic Acids 2012
Human leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells
JH Cho, JS Kim, MU Lim, HK Min, DH Kwak, JS Ryu, JT Lee, SU Kim, CH Kim, CH Kim, DB Koo, KT Chang, YK Choo
Laboratory animal research 2012
Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig
A Richter, M Kurome, B Kessler, V Zakhartchenko, N Klymiuk, H Nagashima, E Wolf, A Wuensch
BMC Biotechnology 2012
Simplified and versatile method for isolation of high-quality RNA from pancreas
M Griffin, M Abu-El-Haija, M Abu-El-Haija, T Rokhlina, A Uc
BioTechniques 2012
CGRP Induction in Cystic Fibrosis Airways Alters The Submucosal Gland Progenitor Cell Niche in Mice
Weiliang Xie, John T. Fisher, Thomas J. Lynch, Meihui Luo, Turan I.A. Evans, Traci L. Neff, Weihong Zhou, Yulong Zhang, Yi Ou, Nigel W. Bunnett, Andrew F. Russo, Michael J. Goodheart, Kalpaj R. Parekh, Xiaoming Liu, John F. Engelhardt
Journal of Clinical Investigation 2011
Swine Models of Cystic Fibrosis Reveal Male Reproductive Tract Phenotype at Birth1
F Pierucci-Alves, V Akoyev, JC 3rd, LH Wang, KS Janardhan, BD Schultz
Biology of reproduction 2011
Genetically manipulated mouse models of lung disease: potential and pitfalls
RM Baron, AJ Choi, CA Owen, AM Choi
American journal of physiology. Lung cellular and molecular physiology 2011
Cystic Fibrosis
MD Amaral, K Kunzelmann
Cystic Fibrosis 2011
Defective Fluid Secretion from Submucosal Glands of Nasal Turbinates from CFTR-/- and CFTRΔF508/ΔF508 Pigs
HJ Cho, NS Joo, JJ Wine, N Vij
PloS one 2011
The ΔF508 Mutation Causes CFTR Misprocessing and Cystic Fibrosis-Like Disease in Pigs
LS Ostedgaard, DK Meyerholz, JH Chen, AA Pezzulo, PH Karp, T Rokhlina, SE Ernst, RA Hanfland, LR Reznikov, PS Ludwig, MP Rogan, GJ Davis, CL Dohrn, C Wohlford-Lenane, PJ Taft, MV Rector, E Hornick, BS Nassar, M Samuel, Y Zhang, SS Richter, A Uc, J Shilyansky, RS Prather, PB McCray, J Zabner, MJ Welsh, DA Stoltz
Science Translational Medicine 2011
Genetic therapies for cystic fibrosis lung disease
PL Sinn, RM Anthony, PB McCray
Human Molecular Genetics 2011
Sequential targeting of CFTR by BAC vectors generates a novel pig model of cystic fibrosis
N Klymiuk, L Mundhenk, K Kraehe, A Wuensch, S Plog, D Emrich, MC Langenmayer, M Stehr, A Holzinger, C Kröner, A Richter, B Kessler, M Kurome, M Eddicks, H Nagashima, K Heinritzi, AD Gruber, E Wolf
Journal of Molecular Medicine 2011
New animal models of cystic fibrosis: what are they teaching us?
NW Keiser, JF Engelhardt
Current Opinion in Pulmonary Medicine 2011
Strategies for selection marker-free swine transgenesis using the Sleeping Beauty transposon system
DF Carlson, JR Garbe, W Tan, MJ Martin, JR Dobrinsky, PB Hackett, KJ Clark, SC Fahrenkrug
Transgenic Research 2011
Functional characterization of the ER stress induced X-box-binding protein-1 (Xbp-1) in the porcine system
JY Zhang, KS Lee, JS Kim, BS Song, DI Jin, DB Koo, K Yu
BMC Molecular Biology 2011
Genetic modifications of pigs for medicine and agriculture
JJ Whyte, RS Prather
Molecular Reproduction and Development 2011
An Evolved Adeno-associated Viral Variant Enhances Gene Delivery and Gene Targeting in Neural Stem Cells
JH Jang, JT Koerber, JS Kim, P Asuri, T Vazin, M Bartel, A Keung, I Kwon, KI Park, DV Schaffer
Molecular Therapy 2011
An update on targeted gene repair in mammalian cells: methods and mechanisms
NM Jensen, T Dalsgaard, M Jakobsen, RR Nielsen, CB Sørensen, L Bolund, TG Jensen
Journal of Biomedical Science 2011
An improved PCR strategy for fast screening of specific and random integrations in rAAV-mediated gene targeted cell clones
Y Luo, L Bolund, CB Sørensen
BMC Research Notes 2011
Efficient immunoglobulin gene disruption and targeted replacement in rabbit using zinc finger nucleases
T Flisikowska, IS Thorey, S Offner, F Ros, V Lifke, B Zeitler, O Rottmann, A Vincent, L Zhang, S Jenkins, H Niersbach, AJ Kind, PD Gregory, AE Schnieke, J Platzer
PloS one 2011
Efficient production of Fah-null heterozygote pigs by chimeric adeno-associated virus-mediated gene knockout and somatic cell nuclear transfer
RD Hickey, JB Lillegard, JE Fisher, TJ McKenzie, SE Hofherr, MJ Finegold, SL Nyberg, M Grompe
Hepatology 2011
Pig gene knockout by rAAV-mediated homologous recombination: comparison of BRCA1 gene knockout efficiency in Yucatan and Göttingen fibroblasts with slightly different target sequences
Y Luo, L Bolund, CB Sørensen
Transgenic Research 2011
Concentration of the antibacterial precursor thiocyanate in cystic fibrosis airway secretions
D Lorentzen, L Durairaj, AA Pezzulo, Y Nakano, J Launspach, DA Stoltz, G Zamba, PB McCray, J Zabner, MJ Welsh, WM Nauseef, B Bánfi
Free radical biology & medicine 2011
Endogenous surface expression of ΔF508-CFTR mediates cAMP-stimulated Cl − current in  CFTR ΔF508/ΔF508 pig thyroid epithelial cells: porcine ΔF508-CFTR mediates cAMP-stimulated Cl − current
Y Li, S Ganta, P Fong
Experimental Physiology 2011
Comparison of potency between histone deacetylase inhibitors trichostatin A and valproic acid on enhancing in vitro development of porcine somatic cell nuclear transfer embryos
YJ Kim, KS Ahn, M Kim, H Shim
In vitro cellular & developmental biology. Animal 2011
Hyposecretion of Fluid from Tracheal Submucosal Glands of CFTR–deficient Pigs
Nam Soo Joo, Hyungju Cho, Monal Khansaheb, Jeffrey J. Wine
Journal of Clinical Investigation 2010
Pathology of Gastrointestinal Organs in a Porcine Model of Cystic Fibrosis
DK Meyerholz, DA Stoltz, AA Pezzulo, MJ Welsh
The American Journal of Pathology 2010
Pigs and humans with cystic fibrosis have reduced insulin-like growth factor 1 (IGF1) levels at birth
MP Rogan, LR Reznikov, AA Pezzulo, ND Gansemer, M Samuel, RS Prather, J Zabner, DC Fredericks, PB McCray, MJ Welsh, DA Stoltz
Proceedings of the National Academy of Sciences 2010
Respiratory Syncytial Virus Engineered To Express the Cystic Fibrosis Transmembrane Conductance Regulator Corrects the Bioelectric Phenotype of Human Cystic Fibrosis Airway Epithelium In Vitro
AR Kwilas, MA Yednak, L Zhang, R Liesman, PL Collins, RJ Pickles, ME Peeples
Journal of virology 2010
Cystic Fibrosis Pigs Develop Lung Disease and Exhibit Defective Bacterial Eradication at Birth
DA Stoltz, DK Meyerholz, AA Pezzulo, S Ramachandran, MP Rogan, GJ Davis, RA Hanfland, C Wohlford-Lenane, CL Dohrn, JA Bartlett, GA 4th, EH Chang, PJ Taft, PS Ludwig, M Estin, EE Hornick, JL Launspach, M Samuel, T Rokhlina, PH Karp, LS Ostedgaard, A Uc, TD Starner, AR Horswill, KA Brogden, RS Prather, SS Richter, J Shilyansky, PB McCray, J Zabner, MJ Welsh
Science Translational Medicine 2010
Sequencing and characterization of the porcine α-galactosidase A gene: towards the generation of a porcine model for Fabry disease
M Yoshimitsu, K Higuchi, X Fan, S Takao, JA Medin, C Tei, T Takenaka
Molecular Biology Reports 2010
Lentiviral Vectors and Cystic Fibrosis Gene Therapy
S Castellani, M Conese
Viruses 2010
Loss of Anion Transport without Increased Sodium Absorption Characterizes Newborn Porcine Cystic Fibrosis Airway Epithelia
JH Chen, DA Stoltz, PH Karp, SE Ernst, AA Pezzulo, TO Moninger, MV Rector, LR Reznikov, JL Launspach, K Chaloner, J Zabner, MJ Welsh
Cell 2010
Targeted disruption of the porcine immunoglobulin kappa light chain locus
J Ramsoondar, M Mendicino, C Phelps, T Vaught, S Ball, J Monahan, S Chen, A Dandro, J Boone, P Jobst, A Vance, N Wertz, I Polejaeva, J Butler, Y Dai, D Ayares, K Wells
Transgenic Research 2010
Generation of antibody- and B cell-deficient pigs by targeted disruption of the J-region gene segment of the heavy chain locus
M Mendicino, J Ramsoondar, C Phelps, T Vaught, S Ball, T LeRoith, J Monahan, S Chen, A Dandro, J Boone, P Jobst, A Vance, N Wertz, Z Bergman, XZ Sun, I Polejaeva, J Butler, Y Dai, D Ayares, K Wells
Transgenic Research 2010
Transgenic pigs as models for translational biomedical research
B Aigner, S Renner, B Kessler, N Klymiuk, M Kurome, A Wünsch, E Wolf
Journal of Molecular Medicine 2010
RNA Interference in Pigs: Comparison of RNAi Test Systems and Expression Vectors
C Merkl, S Leuchs, A Saalfrank, A Kind, A Schnieke
Molecular Biotechnology 2010
High efficiency of BRCA1 knockout using rAAV-mediated gene targeting: developing a pig model for breast cancer
Y Luo, J Li, Y Liu, L Lin, Y Du, S Li, H Yang, G Vajta, H Callesen, L Bolund, CB Sørensen
Transgenic Research 2010
Altered ion transport by thyroid epithelia from CFTR −/− pigs suggests mechanisms for hypothyroidism in cystic fibrosis: Function of CFTR in thyroid of novel porcine model of cystic fibrosis
H Li, S Ganta, P Fong
Experimental Physiology 2010
Potential of anti-inflammatory treatment for cystic fibrosis lung disease.
Taylor-Cousar JL, Von Kessel KA, Young R, Nichols DP
Journal of inflammation research 2010
Substance P stimulates human airway submucosal gland secretion mainly via a CFTR-dependent process
1. Jae Young Choi 2. Monal Sonecha 3. Nam Soo Joo 4. Mauri E. Krouse 5. Robert C. Robbins 6. David Weill 7. Jeffrey J. Wine.
Journal of Clinical Investigation 2009
Hemizygous minipigs produced by random gene insertion and handmade cloning express the Alzheimer's disease-causing dominant mutation APPsw
PM Kragh, AL Nielsen, J Li, Y Du, L Lin, M Schmidt, IB Bøgh, IE Holm, JE Jakobsen, MG Johansen, S Purup, L Bolund, G Vajta, AL Jørgensen
Transgenic Research 2009
Pulmonary surfactant: an immunological perspective
ZC Chroneos, Z Sever-Chroneos, VL Shepherd
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2009
Progress and prospects: techniques for site-directed mutagenesis in animal models
Z Yan, X Sun, JF Engelhardt
Gene Therapy 2009
Indexing TNF-αgene expression using a gene-targeted reporter cell line
Z Yan, D Lei-Butters, JF Engelhardt, GH Leno
BMC Biology 2009
Optimization of square-wave electroporation for transfection of porcine fetal fibroblasts
JW Ross, JJ Whyte, J Zhao, M Samuel, KD Wells, RS Prather
Transgenic Research 2009
Genomic, tissue expression, and protein characterization of pCLCA1, a putative modulator of cystic fibrosis in the pig
S Plog, L Mundhenk, N Klymiuk, AD Gruber
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009
Significant improvement in cloning efficiency of an inbred miniature pig by histone deacetylase inhibitor treatment after somatic cell nuclear transfer
J Zhao, JW Ross, Y Hao, LD Spate, EM Walters, MS Samuel, A Rieke, CN Murphy, RS Prather
Biology of reproduction 2009
Highlights of a workshop to discuss targeting inflammation in cystic fibrosis
KH Banner, HD Jonge, S Elborn, E Growcott, E Gulbins, M Konstan, R Moss, C Poll, SH Randell, AG Rossi, L Thomas, D Waltz
Journal of Cystic Fibrosis 2009
Generation of transgenic non-human primates with germline transmission
E Sasaki, H Suemizu, A Shimada, K Hanazawa, R Oiwa, M Kamioka, I Tomioka, Y Sotomaru, R Hirakawa, T Eto, S Shiozawa, T Maeda, M Ito, R Ito, C Kito, C Yagihashi, K Kawai, H Miyoshi, Y Tanioka, N Tamaoki, S Habu, H Okano, T Nomura
Nature 2009
Genetic manipulation in pigs.
Sachs DH, Galli C
Current Opinion in Organ Transplantation 2009
AAV Targeted Disruption of the CFTR Gene in Cloned Ferrets
Xingshen Sun; Ziying Yan; Yaling Yi; Ziyi Li; Diana Lei; Christopher S. Rogers; Juan Chen; Michael J. Welsh; Gregory H. Leno; and John F. Engelhardt
Journal of Clinical Investigation 2008
Analysis of Adeno-associated Virus Progenitor Cell Transduction in Mouse Lung
X Liu, M Luo, C Guo, Z Yan, Y Wang, DC Lei-Butters, JF Engelhardt
Molecular Therapy 2008
CFTR and defective endocytosis: new insights in the renal phenotype of cystic fibrosis
F Jouret, O Devuyst
Pflügers Archiv - European Journal of Physiology 2008
The porcine lung as a potential model for cystic fibrosis
CS Rogers, WM Abraham, KA Brogden, JF Engelhardt, JT Fisher, PB McCray, G McLennan, DK Meyerholz, E Namati, LS Ostedgaard, RS Prather, JR Sabater, DA Stoltz, J Zabner, MJ Welsh
American journal of physiology. Lung cellular and molecular physiology 2008
Transgene expression of enhanced green fluorescent protein in cloned rabbits generated from in vitro-transfected adult fibroblasts
S Li, Y Guo, J Shi, C Yin, F Xing, L Xu, C Zhang, T Liu, Y Li, H Li, L Du, X Chen
Transgenic Research 2008
Disruption of the CFTR Gene Produces a Model of Cystic Fibrosis in Newborn Pigs
CS Rogers, DA Stoltz, DK Meyerholz, LS Ostedgaard, T Rokhlina, PJ Taft, MP Rogan, AA Pezzulo, PH Karp, OA Itani, AC Kabel, CL Wohlford-Lenane, GJ Davis, RA Hanfland, TL Smith, M Samuel, D Wax, CN Murphy, A Rieke, K Whitworth, A Uc, TD Starner, KA Brogden, J Shilyansky, PB McCray, J Zabner, RS Prather, MJ Welsh
Science 2008
Production of Prnp −/− goats by gene targeting in adult fibroblasts
C Zhu, B Li, G Yu, J Chen, H Yu, J Chen, X Xu, Y Wu, A Zhang, G Cheng
Transgenic Research 2008

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