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Citations to this article

Adiponectin deficiency increases leukocyte-endothelium interactions via upregulation of endothelial cell adhesion molecules in vivo
Raogo Ouedraogo, … , Barry J. Goldstein, Rosario Scalia
Raogo Ouedraogo, … , Barry J. Goldstein, Rosario Scalia
Published June 1, 2007
Citation Information: J Clin Invest. 2007;117(6):1718-1726. https://doi.org/10.1172/JCI29623.
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Research Article Vascular biology Article has an altmetric score of 3

Adiponectin deficiency increases leukocyte-endothelium interactions via upregulation of endothelial cell adhesion molecules in vivo

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Abstract

This study reports on what we believe are novel mechanism(s) of the vascular protective action of adiponectin. We used intravital microscopy to measure leukocyte-endothelium interactions in adiponectin-deficient (Ad–/–) mice and found that adiponectin deficiency was associated with a 2-fold increase in leukocyte rolling and a 5-fold increase in leukocyte adhesion in the microcirculation. Measurement of endothelial NO (eNO) revealed that adiponectin deficiency drastically reduced levels of eNO in the vascular wall. Immunohistochemistry demonstrated increased expression of E-selectin and VCAM-1 in the vascular endothelium of Ad–/– mice. Systemic administration of the recombinant globular adiponectin domain (gAd) to Ad–/– mice significantly attenuated leukocyte-endothelium interactions and adhesion molecule expression in addition to restoring physiologic levels of eNO. Importantly, prior administration of gAd also protected WT mice against TNF-α–induced leukocyte-endothelium interactions, indicating a pharmacologic action of gAd. Mechanistically, blockade of eNOS with Nω-nitro-l-arginine methyl ester (l-NAME) abolished the inhibitory effect of gAd on leukocyte adhesion, demonstrating the obligatory role of eNOS signaling in the antiinflammatory action of gAd. We believe this is the first demonstration that gAd protects the vasculature in vivo via increased NO bioavailability with suppression of leukocyte-endothelium interactions. Overall, we provide evidence that loss of adiponectin induces a primary state of endothelial dysfunction with increased leukocyte-endothelium adhesiveness.

Authors

Raogo Ouedraogo, Yulan Gong, Brett Berzins, Xiandong Wu, Kalyankar Mahadev, Kelly Hough, Lawrence Chan, Barry J. Goldstein, Rosario Scalia

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 Total
Citations: 2 4 4 8 5 4 5 5 6 5 4 9 11 8 9 9 10 7 1 116
Citation information
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Citations to this article in year 2014 (9)

Title and authors Publication Year
Adiponectin Expression Protects against Angiotensin II-Mediated Inflammation and Accelerated Atherosclerosis
CM van Stijn, J Kim, GD Barish, UJ Tietge, RK Tangirala
PloS one 2014
Oxidative Stress in Obesity: A Critical Component in Human Diseases
L Marseglia, S Manti, G D’Angelo, A Nicotera, E Parisi, GD Rosa, E Gitto, T Arrigo
International journal of molecular sciences 2014
Designer Adiponectin Receptor Agonist Stabilizes Metabolic Function and Prevents Brain Injury Caused by HIV Protease Inhibitors
JK Pepping, L Otvos, E Surmacz, S Gupta, JN Keller, AJ Bruce-Keller
Journal of Neuroimmune Pharmacology 2014
Adiponectin expression and the cardioprotective role of the vitamin D receptor activator paricalcitol and the angiotensin converting enzyme inhibitor enalapril in ApoE-deficient mice
E Suarez-Martinez, K Husain, L Ferder
Therapeutic advances in cardiovascular disease 2014
Pulmonary Arterial Hypertension and Insulin Resistance
DB Bradley
Journal of molecular and genetic medicine : an international journal of biomedical research 2014
Association between Risk Factors for Vascular Dementia and Adiponectin
J Song, WT Lee, KA Park, JE Lee
BioMed Research International 2014
The Role of Adiponectin in Endothelial Dysfunction and Hypertension
E Rojas, D Rodríguez-Molina, P Bolli, ZH Israili, J Faría, E Fidilio, V Bermúdez, M Velasco
Current Hypertension Reports 2014
Adiponectin gene polymorphisms in Egyptian type 2 diabetes mellitus patients with and without diabetic nephropathy
AS El-Shal, HE Zidan, NM Rashad
Molecular Biology Reports 2014
Adiponectin Inhibits Tumor Necrosis Factor- -Induced Vascular Inflammatory Response via Caveolin-Mediated Ceramidase Recruitment and Activation
Y Wang, X Wang, WB Lau, Y Yuan, D Booth, JJ Li, R Scalia, K Preston, E Gao, W Koch, XL Ma
Circulation research 2014

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