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Citations to this article

Inhaled NO as a viable antiadhesive therapy for ischemia/reperfusion injury of distal microvascular beds.
A Fox-Robichaud, … , P Reinhardt, P Kubes
A Fox-Robichaud, … , P Reinhardt, P Kubes
Published June 1, 1998
Citation Information: J Clin Invest. 1998;101(11):2497-2505. https://doi.org/10.1172/JCI2736.
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Research Article Article has an altmetric score of 9

Inhaled NO as a viable antiadhesive therapy for ischemia/reperfusion injury of distal microvascular beds.

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Abstract

Inhaled nitric oxide (NO) is being used more and more in intensive care units as a modality to improve the outcome of patients with pulmonary complications. Our objective was to demonstrate that inhaled NO could impact upon a distally inflamed microvasculature-improving perfusion, leukocyte adhesive interactions, and endothelial dysfunction. Using intravital microscopy to visualize ischemia/reperfusion of postcapillary venules, we were able to demonstrate that the reduction in perfusion, the dramatic increase in leukocyte rolling, adhesion, and emigration, and the endothelial dysfunction could all be significantly abrogated with 80 ppm, but not 20 ppm inhaled NO. Perfusing whole blood directly over an inert P-selectin and CD18 ligand substratum incorporated in a flow chamber recruited the same number of rolling and adhering leukocytes from NO-ventilated and non-NO-ventilated animals, suggesting that inhaled NO was not directly affecting leukocytes. To demonstrate that inhaled NO was actually reaching the peripheral microvasculature in vivo, we applied a NO synthase inhibitor locally to the feline mesentery and demonstrated that the vasoconstriction, as well as leukocyte recruitment, were essentially abolished by inhaled NO, suggesting that a NO-depleted peripheral microvasculature could be replenished with inhaled NO in vivo. Finally, inhaled NO at the same concentration that was effective in ischemia/reperfusion did not affect vascular alterations, leukocyte recruitment, and endothelial dysfunction associated with endotoxemia in the feline mesentery. In conclusion, our data for the first time demonstrate a role for inhaled NO as a therapeutic delivery system to the peripheral microvasculature, showing tremendous efficacy as an antiadhesive, antivasoconstrictive, and antipermeabilizing molecule in NO-depleted tissues, but not normal microvessels or vessels that have an abundance of NO (LPS-treated). The notion that blood borne molecules have NO carrying capacity is conceptually consistent with our observations.

Authors

A Fox-Robichaud, D Payne, S U Hasan, L Ostrovsky, T Fairhead, P Reinhardt, P Kubes

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Total citations by year

Year: 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1995 Total
Citations: 3 2 5 2 4 2 3 4 3 8 10 4 8 3 6 6 16 8 8 7 10 7 10 11 15 16 2 1 184
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Citations to this article in year 2014 (10)

Title and authors Publication Year
A randomized clinical trial testing the anti-inflammatory effects of preemptive inhaled nitric oxide in human liver transplantation
JD Lang, AB Smith, A Brandon, KM Bradley, Y Liu, W Li, DR Crowe, NC Jhala, RC Cross, L Frenette, K Martay, YL Vater, AA Vitin, GA Dembo, DA Dubay, JS Bynon, JM Szychowski, JD Reyes, JB Halldorson, SC Rayhill, AA Dick, R Bakthavatsalam, J Brandenberger, JA Broeckel-Elrod, L Sissons-Ross, T Jordan, LY Chen, A Siriussawakul, DE Eckhoff, RP Patel
PloS one 2014
Is Nitrite the Circulating Endocrine Effector of Remote Ischemic Preconditioning?
P Corti, MT Gladwin
Circulation research 2014
Mechanisms of hepatic ischemia-reperfusion injury and protective effects of nitric oxide
LY Guan, PY Fu, PD Li, ZN Li, HY Liu, MG Xin, W Li
World journal of gastrointestinal surgery 2014
Preventing ischemic brain injury after sudden cardiac arrest using NO inhalation
K Kida, F Ichinose
Critical Care 2014
No Signs of Inflammation during Knee Surgery with Ischemia: A Study Involving Inhaled Nitric Oxide
L Hållström, C Frostell, A Herrlin, E Lindroos, I Lundberg, A Soop
Mediators of Inflammation 2014
Working with nitric oxide and hydrogen sulfide in biological systems
S Yuan, RP Patel, CG Kevil
American journal of physiology. Lung cellular and molecular physiology 2014
Comprehensive Physiology
SN Cheuvront, RW Kenefick
Comprehensive Physiology 2014
Generation of purified nitric oxide from liquid N2O4 for the treatment of pulmonary hypertension in hypoxemic swine
MA Lovich, DH Fine, RJ Denton, MG Wakim, AE Wei, MY Maslov, LG Gamero, GB Vasquez, BJ Johnson, RF Roscigno, RJ Gilbert
Nitric Oxide 2014
A Randomized Clinical Trial Testing the Anti-Inflammatory Effects of Preemptive Inhaled Nitric Oxide in Human Liver Transplantation
JD Lang, AB Smith, A Brandon, KM Bradley, Y Liu, W Li, DR Crowe, NC Jhala, RC Cross, L Frenette, K Martay, YL Vater, AA Vitin, GA Dembo, DA DuBay, JS Bynon, JM Szychowski, JD Reyes, JB Halldorson, SC Rayhill, AA Dick, R Bakthavatsalam, J Brandenberger, JA Broeckel-Elrod, L Sissons-Ross, T Jordan, LY Chen, A Siriussawakul, DE Eckhoff, RP Patel, A Gregson
PloS one 2014
Annual Update in Intensive Care and Emergency Medicine 2014
JL Vincent
2014

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