Adenovirus (Ad)-mediated gene transfer to airway epithelia is inefficient because the apical membrane lacks the receptor activity to bind adenovirus fiber protein. Calcium phosphate (CaPi) precipitates have been used to deliver plasmid DNA to cultured cell lines. However, such precipitates are not effective in many primary cultures or in vivo. Here we show that incorporating recombinant adenovirus into a CaPi coprecipitate markedly enhances transgene expression in cells that are resistant to adenovirus infection. Enhancement requires that the virus be contained in the precipitate and viral proteins are required to increase expression. Ad: CaPi coprecipitates increase gene transfer by increasing fiber-independent binding of virus to cells. With differentiated cystic fibrosis (CF) airway epithelia in vitro, a 20-min application of Ad:CaPi coprecipitates that encode CF transmembrane conductance regulator produced as much CF transmembrane conductance regulator Cl- current as a 24-h application of adenovirus alone. We found that Ad:CaPi coprecipitates also increased transgene expression in mouse lung in vivo; importantly, expression was particularly prominent in airway epithelia. These results suggest a new mechanism for gene transfer that may be applicable to a number of different gene transfer applications and could be of value in gene transfer to CF airway epithelia in vivo.
A Fasbender, J H Lee, R W Walters, T O Moninger, J Zabner, M J Welsh
Title and authors | Publication | Year |
---|---|---|
Inhibition of ULK1/2 and KRASG12C controls tumor growth in preclinical models of lung cancer
Ghazi PC, O'Toole KT, Srinivas Boggaram S, Scherzer MT, Silvis MR, Zhang Y, Bogdan M, Smith BD, Lozano G, Flynn DL, Snyder EL, Kinsey CG, McMahon M |
eLife | 2024 |
Shear stress induces autophagy in Schlemm's canal cells via primary cilia-mediated SMAD2/3 signaling pathway.
Shim MS, Dixon A, Nettesheim A, Perkumas KM, Stamer WD, Sun Y, Liton PB |
2023 | |
Antimicrobial mitochondrial reactive oxygen species induction by lung epithelial metabolic reprogramming.
Wang Y, Kulkarni VV, Pantaleón García J, Leiva-Juárez MM, Goldblatt DL, Gulraiz F, Chen J, Donepudi SR, Lorenzi PL, Wang H, Wong LJ, Tuvim MJ, Evans SE |
bioRxiv : the preprint server for biology | 2023 |
Induction and preliminary characterization of neoplastic pulmonary nodules in a transgenic pig model.
Ghosn M, Elsakka AS, Petre EN, Cheleuitte-Nieves C, Tammela T, Monette S, Ziv E, Schachtschneider KM, Srimathveeravalli G, Yarmohammadi H, Edward Boas F, Solomon SB |
Lung cancer (Amsterdam, Netherlands) | 2023 |
Antimicrobial mitochondrial reactive oxygen species induction by lung epithelial immunometabolic modulation.
Wang Y, Kulkarni VV, Pantaleón García J, Leiva-Juárez MM, Goldblatt DL, Gulraiz F, Vila Ellis L, Chen J, Longmire MK, Donepudi SR, Lorenzi PL, Wang H, Wong LJ, Tuvim MJ, Evans SE |
PLoS pathogens | 2023 |
Reversible Myc hypomorphism identifies a key Myc-dependency in early cancer evolution.
Sodir NM, Pellegrinet L, Kortlever RM, Campos T, Kwon YW, Kim S, Garcia D, Perfetto A, Anastasiou P, Swigart LB, Arends MJ, Littlewood TD, Evan GI |
Nature Communications | 2022 |
Gene Therapy: A Possible Alternative to CFTR Modulators?
J Mercier, M Ruffin, H Corvol, L Guillot |
Frontiers in pharmacology | 2021 |
The deubiquitinase USP16 functions as an oncogenic factor in K-RAS-driven lung tumorigenesis
G Xu, Z Yang, Y Ding, Y Liu, L Zhang, B Wang, M Tang, T Jing, K Jiao, X Xu, Z Chen, L Xiang, C Xu, Y Fu, X Zhao, W Jin, Y Liu |
Oncogene | 2021 |
Mineralized polyplexes for gene delivery: Improvement of transfection efficiency as a consequence of calcium incubation and not mineralization
TA Dick, H Uludağ |
Materials science & engineering. C, Materials for biological applications | 2021 |
The coxsackievirus and adenovirus receptor: virological and biological beauty
KJ Excoffon |
FEBS Letters | 2020 |
Mutationally-activated PI3’-kinase-α promotes de-differentiation of lung tumors initiated by the BRAFV600E oncoprotein kinase
JE van Veen, M Scherzer, J Boshuizen, M Chu, A Liu, A Landman, S Green, C Trejo, M McMahon |
eLife | 2019 |
Protein Kinase Cι and Wnt/β-Catenin Signaling: Alternative Pathways to Kras/Trp53-Driven Lung Adenocarcinoma
N Yin, Y Liu, A Khoor, X Wang, EA Thompson, M Leitges, V Justilien, C Weems, NR Murray, AP Fields |
Cancer Cell | 2019 |
Cystic Fibrosis Gene Therapy: Looking Back, Looking Forward
A Cooney, P McCray, P Sinn |
Genes & development | 2018 |
Therapeutic Potential of Biomineralization-Based Engineering
X Wang, Y Xiao, H Hao, Y Zhang, X Xu, R Tang |
2018 | |
Myc Cooperates with Ras by Programming Inflammation and Immune Suppression
RM Kortlever, NM Sodir, CH Wilson, DL Burkhart, L Pellegrinet, LB Swigart, TD Littlewood, GI Evan |
Cell | 2017 |
p53 loss does not permit escape from BrafV600E-induced senescence in a mouse model of lung cancer
S Garnett, KL Dutchak, RV McDonough, D Dankort |
Oncogene | 2017 |
Ect2-Dependent rRNA Synthesis Is Required for KRAS-TRP53-Driven Lung Adenocarcinoma
V Justilien, SA Ali, L Jamieson, N Yin, AD Cox, CJ Der, NR Murray, AP Fields |
Cancer Cell | 2017 |
Tumor-targeted SN38 inhibits growth of early stage non-small cell lung cancer (NSCLC) in a KRas/p53 transgenic mouse model
AY Deneka, L Haber, MC Kopp, AV Gaponova, AS Nikonova, EA Golemis, AR Amin |
PloS one | 2017 |
Oncolytic Adenovirus Complexes Coated with Lipids and Calcium Phosphate for Cancer Gene Therapy
J Chen, P Gao, S Yuan, R Li, A Ni, L Chu, L Ding, Y Sun, XY Liu, Y Duan |
ACS Nano | 2016 |
Adenofection: A Method for Studying the Role of Molecular Chaperones in Cellular Morphodynamics by Depletion-Rescue Experiments
M Fuchs, MC Boulanger, H Lambert, J Landry, JN Lavoie |
Journal of visualized experiments : JoVE | 2016 |
PIK3CAH1047R Accelerates and Enhances KRASG12D-Driven Lung Tumorigenesis
S Green, CL Trejo, M McMahon |
Cancer research | 2015 |
TP53 Silencing Bypasses Growth Arrest of BRAFV600E-Induced Lung Tumor Cells in a Two-Switch Model of Lung Tumorigenesis
A Shai, D Dankort, J Juan, S Green, M McMahon |
Cancer research | 2015 |
Mouse Genetics
SR Singh, V Coppola |
Mouse Genetics | 2014 |
Mouse Genetics
SR Singh, V Coppola |
Mouse Genetics | 2014 |
Mouse Genetics
SR Singh, V Coppola |
Mouse Genetics | 2014 |
Diminished WNT -> β-catenin -> c-MYC signaling is a barrier for malignant progression of BRAFV600E-induced lung tumors
J Juan, T Muraguchi, G Iezza, RC Sears, M McMahon |
Genes & development | 2014 |
Sheep Lung Segmental Delivery Strategy Demonstrates Adenovirus Priming of Local Lung Responses to Bacterial LPS and the Role of Elafin as a Response Modulator
TI Brown, DS Collie, DJ Shaw, NM Rzechorzek, JM Sallenave, JA Chabalgoity |
PloS one | 2014 |
Genetically engineered mouse models for epithelial ovarian cancer: Are we there yet?
VM Howell |
Seminars in Cell & Developmental Biology | 2014 |
A single extra copy of Dscr1 improves survival of mice developing spontaneous lung tumors through suppression of tumor angiogenesis
J Shin, JC Lee, KH Baek |
Cancer Letters | 2014 |
Diminished WNT → β-catenin → c-MYC signaling is a barrier for malignant progression of BRAFV600E-induced lung tumors
J Juan, T Muraguchi, G Iezza, RC Sears, M McMahon |
Genes & development | 2014 |
Thrombospondin-1 mediates oncogenic ras-induced senescence in pre-malignant lung tumors
Kwan-hyuck Baek, Dongha Bhang, Alexander Zaslavsky, Liang-Chuan Wang, Anil Vachani, Carla Kim, Steven Albelda, Gerard I. Evan, Sandra Ryeom |
Journal of Clinical Investigation | 2013 |
Protein kinase Cα suppresses Kras-mediated lung tumor formation through activation of a p38 MAPK-TGFβ signaling axis
KS Hill, E Erdogan, A Khoor, MP Walsh, M Leitges, NR Murray, AP Fields |
Oncogene | 2013 |
Mutationally Activated PIK3CAH1047R Cooperates with BRAFV600E to Promote Lung Cancer Progression
CL Trejo, S Green, V Marsh, EA Collisson, G Iezza, WA Phillips, M McMahon |
Cancer research | 2013 |
IDO is a nodal pathogenic driver of lung cancer and metastasis development
C Smith, MY Chang, KH Parker, DW Beury, JB DuHadaway, HE Flick, J Boulden, E Sutanto-Ward, AP Soler, LD Laury-Kleintop, L Mandik-Nayak, R Metz, S Ostrand-Rosenberg, GC Prendergast, AJ Muller |
Cancer Discovery | 2012 |
Overcoming the stromal barrier: technologies to optimize drug delivery in pancreatic cancer
A Dimou, KN Syrigos, MW Saif |
Therapeutic advances in medical oncology | 2012 |
Methods in Enzymology
JD Stone, AS Chervin, DH Aggen, DM Kranz |
Protein Engineering for Therapeutics Part B | 2012 |
Biomineralization-Based Virus Shell-Engineering: Towards Neutralization Escape and Tropism Expansion
X Wang, Y Deng, S Li, G Wang, E Qin, X Xu, R Tang, C Qin |
Advanced Healthcare Materials | 2012 |
MEK1/2 Inhibition Elicits Regression of Autochthonous Lung Tumors Induced by KRAS G12D or BRAF V600E
CL Trejo, J Juan, S Vicent, A Sweet-Cordero, M McMahon |
Cancer research | 2012 |
Progression of human bronchioloalveolar carcinoma to invasive adenocarcinoma is modeled in a transgenic mouse model of K-ras-induced lung cancer by loss of the TGF-β type II receptor
AC Borczuk, M Sole, P Lu, J Chen, ML Wilgus, RA Friedman, SM Albelda, CA Powell |
Cancer research | 2011 |
Anionic Liposomes Enhance and Prolong Adenovirus-Mediated Gene Expression in Airway Epitheliain Vitroandin Vivo
Z Zhong, J Han, Y Wan, Z Zhang, X Sun |
Molecular Pharmaceutics | 2011 |
Intracellular Delivery
A Prokop |
2011 | |
Activation of the stress protein response inhibits the STAT1 signalling pathway and iNOS function in alveolar macrophages: role of Hsp90 and Hsp70
M Howard, J Roux, H Lee, B Miyazawa, JW Lee, B Gartland, AJ Howard, MA Matthay, M Carles, JF Pittet |
Thorax | 2010 |
Calcium gluconate in phosphate buffered saline increases gene delivery with adenovirus type 5
MT Ahonen, I Diaconu, S Pesonen, A Kanerva, M Baumann, ST Parviainen, B Spiller, V Cerullo, A Hemminki |
PloS one | 2010 |
Assessing therapeutic responses in Kras mutant cancers using genetically engineered mouse models
M Singh, A Lima, R Molina, P Hamilton, AC Clermont, V Devasthali, JD Thompson, JH Cheng, HB Reslan, CC Ho, TC Cao, CV Lee, MA Nannini, G Fuh, RA Carano, H Koeppen, RX Yu, WF Forrest, GD Plowman, L Johnson |
Nature Biotechnology | 2010 |
Air-assisted intranasal instillation enhances adenoviral delivery to the olfactory epithelium and respiratory tract
P Gau, S Rodriguez, CD Leonardis, P Chen, DM Lin |
Gene Therapy | 2010 |
Influences of electrolytes and glucose on formulation of carbonate apatite nanocrystals for efficient gene delivery to mammalian cells
S Hossain, S Tada, T Akaike, EH Chowdhury |
Analytical Biochemistry | 2010 |
Atypical protein kinase C{iota} is required for bronchioalveolar stem cell expansion and lung tumorigenesis
RP Regala, RK Davis, A Kunz, A Khoor, M Leitges, AP Fields |
Cancer research | 2009 |
Genetically modified adenoviral vector with the protein transduction domain of Tat improves gene transfer to CAR-deficient cells
S Liu, Q Mao, W Zhang, X Zheng, Y Bian, D Wang, H Li, L Chai, J Zhao, H Xia |
Bioscience Reports | 2009 |
Cationic lipid formulations alter the in vivo tropism of AAV2/9 vector in lung
DE Fein, MP Limberis, SF Maloney, JM Heath, JM Wilson, SL Diamond |
Molecular Therapy | 2009 |
Conditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase
M DuPage, AL Dooley, T Jacks |
Nature Protocols | 2009 |
Nano-structured composites based on calcium phosphate for cellular delivery of therapeutic and diagnostic agents
M Zhang, K Kataoka |
Nano Today | 2009 |
Thoracic Endoscopy: Advances in Interventional Pulmonology
RJ Kruklitis, DH Sterman |
Thoracic Endoscopy: Advances in Interventional Pulmonology | 2008 |
Distinct thresholds govern Myc's biological output in vivo
DJ Murphy, MR Junttila, L Pouyet, A Karnezis, K Shchors, DA Bui, L Brown-Swigart, L Johnson, GI Evan |
Cancer Cell | 2008 |
A simple, lanthanide-based method to enhance the transduction efficiency of adenovirus vectors
GD Palmer, MJ Stoddart, E Gouze, JN Gouze, SC Ghivizzani, RM Porter, CH Evans |
Gene Therapy | 2008 |
Detection of Pulmonary Gene Transfer Using Iodide-124/Positron Emission Tomography
FE Domann, G Niu |
Cancer Imaging | 2008 |
Three-Dimensional Structure of Canine Adenovirus Serotype 2 Capsid
G Schoehn, ME Bakkouri, CM Fabry, O Billet, LF Estrozi, L Le, DT Curiel, AV Kajava, RW Ruigrok, EJ Kremer |
Journal of virology | 2008 |
Dendritic Cell Function After Gene Transfer with Adenovirus-calcium Phosphate Co-precipitates
MP Seiler, S Gottschalk, V Cerullo, M Ratnayake, VP Mane, C Clarke, DJ Palmer, P Ng, CM Rooney, B Lee |
Molecular Therapy | 2007 |
A new mouse model to explore the initiation, progression, and therapy of BRAFV600E-induced lung tumors
D Dankort, E Filenova, M Collado, M Serrano, K Jones, M McMahon |
Genes & development | 2007 |
Calcium phosphate coprecipitation greatly enhances transduction of cardiac myocytes and vascular smooth muscle cells by lentivirus vectors
T Sakoda, N Kasahara, L Kedes, M Ohyanagi |
Experimental and clinical cardiology | 2007 |
High performance DNA nano-carriers of carbonate apatite: multiple factors in regulation of particle synthesis and transfection efficiency
EH Chowdhury, T Akaike |
International Journal of Nanomedicine | 2007 |
Novel Therapies for the Treatment of Cystic Fibrosis: New Developments in Gene and Stem Cell Therapy
V Sueblinvong, BT Suratt, DJ Weiss |
Clinics in Chest Medicine | 2007 |
Human Adenovirus Modulates Surfactant Phospholipid Trafficking
OL Miakotina, DM McCoy, L Shi, DC Look, RK Mallampalli |
Traffic | 2007 |
High performance DNA nano-carriers of carbonate apatite: Multiple factors in regulation of particle synthesis and transfection effi ciency
EH Chowdhury, T Akaike |
International Journal of Nanomedicine | 2007 |
Can Myoglobin Expression in Pancreatic Beta Cells Improve Insulin Secretion Under Hypoxia? An Exploratory Study With Transgenic Porcine Islets
HK Tilakaratne, B Yang, SK Hunter, ME Andracki, VG Rodgers |
Artificial Organs | 2007 |
Paraoxonase-2 deficiency enhances Pseudomonas aeruginosa quorum sensing in murine tracheal epithelia
DA Stoltz, EA Ozer, CJ Ng, JM Yu, ST Reddy, AJ Lusis, N Bourquard, MR Parsek, J Zabner, DM Shih |
American journal of physiology. Lung cellular and molecular physiology | 2007 |
A New Binding Motif for the Transcriptional Repressor REST Uncovers Large Gene Networks Devoted to Neuronal Functions
SJ Otto, SR McCorkle, J Hover, C Conaco, JJ Han, S Impey, GS Yochum, JJ Dunn, RH Goodman, G Mandel |
The Journal of neuroscience : the official journal of the Society for Neuroscience | 2007 |
Reciprocal actions of REST and a microRNA promote neuronal identity
C Conaco, S Otto, JJ Han, G Mandel |
Proceedings of the National Academy of Sciences | 2006 |
pH-sensing nano-crystals of carbonate apatite: Effects on intracellular delivery and release of DNA for efficient expression into mammalian cells
EH Chowdhury, A Maruyama, A Kano, M Nagaoka, M Kotaka, S Hirose, M Kunou, T Akaike |
Gene | 2006 |
Blockade of TNF-α Decreases Both Inflammation and Efficacy of Intrapulmonary Ad.IFNβ Immunotherapy in an Orthotopic Model of Bronchogenic Lung Cancer
MJ Wilderman, S Kim, CT Gillespie, J Sun, V Kapoor, A Vachani, DH Sterman, LR Kaiser, SM Albelda |
Molecular Therapy | 2006 |
Principles of Molecular Medicine
MS Runge, C Patterson |
2006 | |
Gene Therapy for Lung Diseases
Alton EW, Davies JC, Geddes DM |
2006 | |
The role of the extracellular domain in the biology of the coxsackievirus and adenovirus receptor
KJ Excoffon, GL Traver, J Zabner |
American journal of respiratory cell and molecular biology | 2005 |
Gene transfer of CFTR to airway epithelia: low levels of expression are sufficient to correct Cl − transport and overexpression can generate basolateral CFTR
SL Farmen, PH Karp, P Ng, DJ Palmer, DR Koehler, J Hu, AL Beaudet, J Zabner, MJ Welsh |
American journal of physiology. Lung cellular and molecular physiology | 2005 |
Antimicrobial Activity of Murine Lung Cells against Staphylococcus aureus Is Increased In Vitro and In Vivo after Elafin Gene Transfer
JW McMichael, AI Maxwell, K Hayashi, K Taylor, WA Wallace, JR Govan, JR Dorin, JM Sallenave |
Infection and immunity | 2005 |
Gene delivery of l-caldesmon protects cytoskeletal cell membrane integrity against adenovirus infection independently of myosin ATPase and actin assembly
K Haxhinasto, A Kamath, K Blackwell, J Bodmer, JV Heukelom, A English, EW Bai, AB Moy |
American journal of physiology. Cell physiology | 2004 |
Adenovirus-Mediated Erythropoietin Production by Airway Epithelia Is Enhanced by Apical Localization of the Coxsackie–Adenovirus Receptor in Vivo
B Davis, J Nguyen, D Stoltz, D Depping, KJ Excoffon, J Zabner |
Molecular Therapy | 2004 |
High-efficiency gene delivery for expression in mammalian cells by nanoprecipitates of Ca–Mg phosphate
EH Chowdhury, M Kunou, M Nagaoka, AK Kundu, T Hoshiba, T Akaike |
Gene | 2004 |
Dramatic effect of Mg2+ on transfecting mammalian cells by DNA/calcium phosphate precipitates
EH Chowdhury, K Megumi, I Harada, AK Kundu, T Akaike |
Analytical Biochemistry | 2004 |
Fibronectin in collaboration with Mg2+ enhances transgene expression by calcium phosphate coprecipitates
EH Chowdhury, FT Zohra, S Tada, C Kitamura, T Akaike |
Analytical Biochemistry | 2004 |
Lung Biology in Health and Disease: Past, Present and Future
R Kruklitis, D Sterman |
Lung Biology in Health and Disease: Experimental Models and Therapeutic Potential | 2004 |
Fluorescently Labeled Adenovirus with pIX-EGFP for Vector Detection
LP Le, M Everts, IP Dmitriev, JG Davydova, M Yamamoto, DT Curiel |
Molecular imaging | 2004 |
Adenoviral Gene Delivery of Elafin and Secretory Leukocyte Protease Inhibitor Attenuates NF-κB-Dependent Inflammatory Responses of Human Endothelial Cells and Macrophages to Atherogenic Stimuli
PA Henriksen, M Hitt, Z Xing, J Wang, C Haslett, RA Riemersma, DJ Webb, YV Kotelevtsev, JM Sallenave |
Journal of immunology (Baltimore, Md. : 1950) | 2004 |
Incorporation of calcium phosphate enhances recombinant adeno-associated virus-mediated gene therapy in diabetic mice
YW Yang, CK Chao |
The Journal of Gene Medicine | 2003 |
Transfecting mammalian cells by DNA/calcium phosphate precipitates: Effect of temperature and pH on precipitation
EH Chowdhury, T Sasagawa, M Nagaoka, AK Kundu, T Akaike |
Analytical Biochemistry | 2003 |
Gene therapy for asthma
P Factor |
Molecular Therapy | 2003 |
The Coxsackie B Virus and Adenovirus Receptor Resides in a Distinct Membrane Microdomain
KJ Excoffon, T Moninger, J Zabner |
Journal of virology | 2003 |
CURRENTSTATUS OFGENETHERAPY FORINHERITEDLUNGDISEASES
RA Driskell, JF Engelhardt |
Annual Review of Physiology | 2002 |
Barriers to and new approaches for gene therapy and gene delivery in cystic fibrosis
S Ferrari, DM Geddes, EW Alton |
Advanced Drug Delivery Reviews | 2002 |
CFTR with a partially deleted R domain corrects the cystic fibrosis chloride transport defect in human airway epithelia in vitro and in mouse nasal mucosa in vivo
LS Ostedgaard, J Zabner, DW Vermeer, T Rokhlina, PH Karp, AA Stecenko, C Randak, MJ Welsh |
Proceedings of the National Academy of Sciences | 2002 |
Adenoviral Vectors for Gene Therapy
D Kirn |
Adenoviral Vectors for Gene Therapy | 2002 |
Gene Therapy in Lung Disease
K Amin |
Gene Therapy in Lung Disease | 2002 |
Gene Therapy for Airway Diseases: Continued Progress toward Identifying and Overcoming Barriers to Efficiency
JM Pilewski |
American journal of respiratory cell and molecular biology | 2002 |
Thixotropic Solutions Enhance Viral-Mediated Gene Transfer to Airway Epithelia
MP Seiler, P Luner, TO Moninger, PH Karp, S Keshavjee, J Zabner |
American journal of respiratory cell and molecular biology | 2002 |
Human adenovirus serotypes 4p and 11p are efficiently expressed in cell lines of neural tumour origin
J Skog, YF Mei, G Wadell |
Journal of General Virology | 2002 |
Delivery of Gene Transfer Vectors to Lung: Obstacles and the Role of Adjunct Techniques for Airway Administration
DJ Weiss |
Molecular Therapy | 2002 |
Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo
F Scherer, M Anton, U Schillinger, J Henke, C Bergemann, A Krüger, B Gänsbacher, C Plank |
Gene Therapy | 2002 |
Gene therapy for cystic fibrosis
JC Davies, DM Geddes, EW Alton |
The Journal of Gene Medicine | 2001 |
EGTA Enhancement of Adenovirus-Mediated Gene Transfer to Mouse Tracheal Epitheliumin Vivo
Q Chu, JA George, M Lukason, SH Cheng, RK Scheule, SJ Eastman |
Human Gene Therapy | 2001 |
Transduction and Apoptosis Induction in the Rat Prostate, Using Adenovirus Vectors
W Kirkman, P Chen, R Schroeder, MR Feneley, R Rodriguez, TJ Wickham, CR King, JT Bruder |
Human Gene Therapy | 2001 |
Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras
EL Jackson, N Willis, K Mercer, RT Bronson, D Crowley, R Montoya, T Jacks, DA Tuveson |
Genes & development | 2001 |
Rapid assessment of adenovirus serum neutralizing antibody titer based on quantitative, morphometric evaluation of capsid binding and intracellular trafficking: population analysis of adenovirus capsid association with cells is predictive of adenovirus infectivity
T Vincent, BG Harvey, SM Hogan, CJ Bailey, RG Crystal, PL Leopold |
Journal of virology | 2001 |
Cationic Polymer and Lipids Augment Adenovirus-Mediated Gene Transfer to Cerebral Arteries In Vivo
K Toyoda, H Nakane, DD Heistad |
Journal of Cerebral Blood Flow & Metabolism | 2001 |
Trafficking and Propagation of Canine Adenovirus Vectors Lacking a Known Integrin-Interacting Motif
M Chillon, EJ Kremer |
Human Gene Therapy | 2001 |
Transfer of high copy number plasmid into mammalian cells by calcium phosphate transfection
P Batard, M Jordan, F Wurm |
Gene | 2001 |
Gene Therapy for Acute Diseases
P Factor |
Molecular Therapy | 2001 |
Adenovirus Calcium Phosphate Coprecipitates Enhance Squamous Cell Carcinoma Gene Transfer:
SM Yi, JH Lee, S Graham, J Zabner, MJ Welsh |
The Laryngoscope | 2001 |
Gene Therapy for Acute and Acquired Diseases
P Factor |
2001 | |
Apical Localization of the Coxsackie-Adenovirus Receptor by Glycosyl-Phosphatidylinositol Modification Is Sufficient for Adenovirus-Mediated Gene Transfer through the Apical Surface of Human Airway Epithelia
RW Walters, W van't Hof, SM Yi, MK Schroth, J Zabner, RG Crystal, MJ Welsh |
Journal of virology | 2001 |
Targeting the urokinase plasminogen activator receptor enhances gene transfer to human airway epithelia
PT Drapkin, CR O'Riordan, SM Yi, JA Chiorini, J Cardella, J Zabner, MJ Welsh |
Journal of Clinical Investigation | 2000 |
Immunomodulation and Adenoviral Gene Transfer to the Lungs of Nonhuman Primates
MK Shean, G Baskin, D Sullivan, J Schurr, DE Cavender, JE Shellito, PO Schwarzenberger, JK Kolls |
Human Gene Therapy | 2000 |
Endocytosis and nuclear trafficking of adeno-associated virus type 2 are controlled by rac1 and phosphatidylinositol-3 kinase activation
S Sanlioglu, PK Benson, J Yang, EM Atkinson, T Reynolds, JF Engelhardt |
Journal of virology | 2000 |
Incorporation of adeno-associated virus in a calcium phosphate coprecipitate improves gene transfer to airway epithelia in vitro and in vivo
RW Walters, D Duan, JF Engelhardt, MJ Welsh |
Journal of virology | 2000 |
Canine adenovirus vectors: an alternative for adenovirus-mediated gene transfer
EJ Kremer, S Boutin, M Chillon, O Danos |
Journal of virology | 2000 |
Studying heterotrimeric G-protein-linked signal transduction using replication-deficient adenoviruses
MM Cummins, P Poronnik, LM O'Mullane, DI Cook |
Immunology and Cell Biology | 2000 |
Progress in adenovirus-mediated gene therapy for cystic fibrosis lung disease
T Matsuse, S Teramoto |
Current Therapeutic Research | 2000 |
Insulin-Like Growth Factor Binding Protein-6 Inhibits the Growth of Human Bronchial Epithelial Cells and Increases in Abundance with All- trans -Retinoic Acid Treatment
N Sueoka, HY Lee, GL Walsh, B Fang, L Ji, JA Roth, R LaPushin, WK Hong, P Cohen, JM Kurie |
American journal of respiratory cell and molecular biology | 2000 |
Update on adenovirus and its vectors
WC Russell |
Journal of General Virology | 2000 |
Calcium phosphate precipitates augment adenovirus-mediated gene transfer to blood vessels in vitro and in vivo
K Toyoda, JJ Andresen, J Zabner, FM Faraci, DD Heistad |
Gene Therapy | 2000 |
TLR4 mutations are associated with endotoxin hyporesponsiveness in humans
NC Arbour, E Lorenz, BC Schutte, J Zabner, JN Kline, M Jones, K Frees, JL Watt, DA Schwartz |
Nature Genetics | 2000 |
Canine Adenovirus Type 2 Attachment and Internalization: Coxsackievirus-Adenovirus Receptor, Alternative Receptors, and an RGD-Independent Pathway
C Soudais, S Boutin, SS Hong, M Chillon, O Danos, JM Bergelson, P Boulanger, EJ Kremer |
Journal of virology | 2000 |
Gene transfer for cystic fibrosis
MJ Welsh |
Journal of Clinical Investigation | 1999 |
Delivery of an Adenovirus Vector in a Calcium Phosphate Coprecipitate Enhances the Therapeutic Index of Gene Transfer to Airway Epithelia
JH Lee, J Zabner, MJ Welsh |
Human Gene Therapy | 1999 |
A chimeric type 2 adenovirus vector with a type 17 fiber enhances gene transfer to human airway epithelia
J Zabner, M Chillon, T Grunst, TO Moninger, BL Davidson, R Gregory, D Armentano |
Journal of virology | 1999 |
Reciprocal Enhancement of Gene Transfer by Combinatorial Adenovirus Transduction and Plasmid DNA Transfection in Vitro and in Vivo
EJ Dunphy, RA Redman, H Herweijer, TP Cripe |
Human Gene Therapy | 1999 |
Enhancement of calcium phosphate-mediated transfection by inclusion of adenovirus in coprecipitates
JH Lee, MJ Welsh |
Gene Therapy | 1999 |
Mechanism by which calcium phosphate coprecipitation enhances adenovirus-mediated gene transfer
RW Walters, MJ Welsh |
Gene Therapy | 1999 |