Thrombus formation in vivo
Bruce Furie, Barbara C. Furie
Bruce Furie, Barbara C. Furie
Published December 1, 2005
Citation Information: J Clin Invest. 2005;115(12):3355-3362. https://doi.org/10.1172/JCI26987.
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Thrombus formation in vivo

Abstract

To examine thrombus formation in a living mouse, new technologies involving intravital videomicroscopy have been applied to the analysis of vascular windows to directly visualize arterioles and venules. After vessel wall injury in the microcirculation, thrombus development can be imaged in real time. These systems have been used to explore the role of platelets, blood coagulation proteins, endothelium, and the vessel wall during thrombus formation. The study of biochemistry and cell biology in a living animal offers new understanding of physiology and pathology in complex biologic systems.

Authors

Bruce Furie, Barbara C. Furie

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Model of P-selectin/PSGL-1–mediated tissue factor accumulation during th...
Model of P-selectin/PSGL-1–mediated tissue factor accumulation during thrombus formation. (A) Leukocyte microparticles (red) circulate constitutively in the blood under resting conditions. These microparticles express tissue factor (TF) and PSGL-1 on their surface. (B) Vessel wall tissue factor is expressed in response to laser-induced injury, leading to platelet activation and the subsequent expression of P-selectin on the stimulated platelets incorporated into the developing thrombus (initiation phase). (C) Blood-borne tissue factor associated with microparticles accumulates on the platelet thrombus through the binding of platelet P-selectin and microparticle PSGL-1. (D) Concentration of blood-borne tissue factor into the thrombus initiates thrombin generation and fibrin clot propagation within the thrombus (propagation phase).