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Citations to this article

Transplantation of spermatogonial stem cells isolated from leukemic mice restores fertility without inducing leukemia
Kazutoshi Fujita, … , Teruhiko Wakayama, Akihiko Okuyama
Kazutoshi Fujita, … , Teruhiko Wakayama, Akihiko Okuyama
Published July 1, 2005
Citation Information: J Clin Invest. 2005;115(7):1855-1861. https://doi.org/10.1172/JCI24189.
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Research Article Reproductive biology

Transplantation of spermatogonial stem cells isolated from leukemic mice restores fertility without inducing leukemia

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Abstract

More than 70% of patients survive childhood leukemia, but chemotherapy and radiation therapy cause irreversible impairment of spermatogenesis. Although autotransplantation of germ cells holds promise for restoring fertility, contamination by leukemic cells may induce relapse. In this study, we isolated germ cells from leukemic mice by FACS sorting. The cell population in the high forward-scatter and low side-scatter regions of dissociated testicular cells from leukemic mice were analyzed by staining for MHC class I heavy chain (H-2Kb/H-2Db) and for CD45. Cells that did not stain positively for H-2Kb/H-2Db and CD45 were sorted as the germ cell–enriched fraction. The sorted germ cell–enriched fractions were transplanted into the testes of recipient mice exposed to alkylating agents. Transplanted germ cells colonized, and recipient mice survived. Normal progeny were produced by intracytoplasmic injection of sperm obtained from recipient testes. When unsorted germ cells from leukemic mice were transplanted into recipient testes, all recipient mice developed leukemia. The successful birth of offspring from recipient mice without transmission of leukemia to the recipients indicates the potential of autotransplantation of germ cells sorted by FACS to treat infertility secondary to anticancer treatment for childhood leukemia.

Authors

Kazutoshi Fujita, Hiroshi Ohta, Akira Tsujimura, Tetsuya Takao, Yasushi Miyagawa, Shingo Takada, Kiyomi Matsumiya, Teruhiko Wakayama, Akihiko Okuyama

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Total citations by year

Year: 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2006 2005 Total
Citations: 1 1 2 2 1 4 3 2 1 3 5 3 1 3 3 3 1 1 1 41
Citation information
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Citations to this article (41)

Title and authors Publication Year
Postnatal development of mouse spermatogonial stem cells as determined by immunophenotype, regenerative capacity, and long-term culture-initiating ability: a model for practical applications.
Song Y, Zhang X, Desmarais JA, Nagano M
Scientific Reports 2024
Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells.
Munyoki SK, Orwig KE
Methods in molecular biology (Clifton, N.J.) 2023
Fertility Preservation and Restoration Options for Pre-Pubertal Male Cancer Patients: Current Approaches
Eugeni E, Arato I, Del Sordo R, Sidoni A, Garolla A, Ferlin A, Calafiore R, Brancorsini S, Mancuso F, Luca G
Frontiers in Endocrinology 2022
Efficacy of Ficus carica leaf extract on morphological and molecular behavior of mice germ stem cells
Makoolati Z, Bahrami H, Zamanzadeh Z, Mahaldashtian M, Moulazadeh A, Ebrahimi L, Naghdi M
2022
Disease-inducing potential of two leukemic cell lines in a xenografting model
DD Manavella, S Herraiz, M Soares, A Buigues, A Pellicer, J Donnez, C Díaz-García, MM Dolmans
Journal of Assisted Reproduction and Genetics 2021
A microfluidic approach to rapid sperm recovery from heterogeneous cell suspensions
SA Vasilescu, S Khorsandi, L Ding, SR Bazaz, R Nosrati, D Gook, ME Warkiani
Scientific Reports 2021
Spermatogonial Stem Cell Culture in Oncofertility
S David, KE Orwig
The Urologic clinics of North America 2020
Autotransplantation in combination with orthodontic treatment
R Hariri, EE Alzoubi
Journal of Orthodontic Science 2019

Role of stem cells in fertility preservation: current insights


M Vermeulen, MG Giudice, FD Vento, C Wyns
Stem cells and cloning : advances and applications 2019
The Progresses of Spermatogonial Stem Cells Sorting Using Fluorescence-Activated Cell Sorting
Y Cai, J Wang, K Zou
Stem Cell Reviews and Reports 2019
Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation
J Tian, K Ma, C Pei, S Zhang, X Li, Y Zhou, B Yan, H Wang, L Ma
Stem Cell Research & Therapy 2019
Tissue Engineering to Improve Immature Testicular Tissue and Cell Transplantation Outcomes: One Step Closer to Fertility Restoration for Prepubertal Boys Exposed to Gonadotoxic Treatments
FD Vento, M Vermeulen, F de Michele, M Giudice, J Poels, A des Rieux, C Wyns
International journal of molecular sciences 2018
Successful Human Spermatogonial Stem Cells Homing in Recipient Mouse Testis after In Vitro Transplantation and Organ Culture
Mahdi Mohaqiq, Mansoureh Movahedin, Zohreh Mazaheri, Naser Amirjannati
Cell Journal (Yakhteh) 2018
Elimination of mouse tumor cells from neonate spermatogonial cells utilizing cisplatin-entrapped folic acid-conjugated poly(lactic-co-glycolic acid) nanoparticles in vitro
R Shabani, M Ashjari, K Ashtari, F Izadyar, B Behnam, S Khoei, M Asghari-Jafarabadi, M Koruji
International Journal of Nanomedicine 2018
Progress and future prospect of in vitro spermatogenesis
F Ibtisham, J Wu, M Xiao, L An, Z Banker, A Nawab, Y Zhao, GH Li
Oncotarget 2017
Long-term health in recipients of transplanted in vitro propagated spermatogonial stem cells
CL Mulder, LA Catsburg, Y Zheng, CM de Winter-Korver, SK van Daalen, M van Wely, S Pals, S Repping, AM van Pelt
Human Reproduction 2017
Alteration of spermatogenesis following spermatogonial stem cells transplantation in testicular torsion-detorsion mice
S Azizollahi, R Aflatoonian, MA Gilani, B Behnam, N Tajik, M Asghari-Jafarabadi, HR Asgari, M Koruji
Journal of Assisted Reproduction and Genetics 2016
Granulocyte colony-stimulating factor prevents loss of spermatogenesis after sterilizing busulfan chemotherapy
R Benavides-Garcia, R Joachim, NA Pina, KN Mutoji, MA Reilly, BP Hermann
Fertility and Sterility 2015
Is transplantation of a few leukemic cells inside an artificial ovary able to induce leukemia in an experimental model?
M Soares, P Saussoy, K Sahrari, CA Amorim, J Donnez, MM Dolmans
Journal of Assisted Reproduction and Genetics 2015
Cryopreservation of testis tissues and in vitro spermatogenesis
T Yokonishi, T Ogawa
Reproductive Medicine and Biology 2015
Germline stem cells: toward the regeneration of spermatogenesis
H Valli, BT Phillips, G Shetty, JA Byrne, AT Clark, ML Meistrich, KE Orwig
Fertility and Sterility 2014
Fluorescence- and magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cells☆
H Valli, M Sukhwani, SL Dovey, KA Peters, J Donohue, CA Castro, T Chu, GR Marshall, KE Orwig
Fertility and Sterility 2014
Preserving children’s fertility: two tales about children’s right to an open future and the margins of parental obligations
D Cutas, K Hens
Medicine, Health Care and Philosophy 2014
Optimizing cryopreservation of human spermatogonial stem cells: comparing the effectiveness of testicular tissue and single cell suspension cryopreservation
P Yango, E Altman, JF Smith, PC Klatsky, ND Tran
Fertility and Sterility 2014
Characterization of human spermatogonial stem cell markers in fetal, pediatric, and adult testicular tissues
E Altman, P Yango, R Moustafa, JF Smith, PC Klatsky, ND Tran
Reproduction 2014
Eliminating malignant contamination from therapeutic human spermatogonial stem cells
Serena Dovey, Hanna Valli, Brian Hermann, Meena Sukhwani, Julia Donohue, Carlos Castro, Tianjiao Chu, Joseph Sanfilippo, Kyle Orwig
Journal of Clinical Investigation 2013
Propagation of Adult SSCs: From Mouse to Human
LA Martin, M Seandel
BioMed Research International 2013
The effects of poly L-lactic acid nanofiber scaffold on mouse spermatogonial stem cell culture
N Eslahi, MR Hadjighassem, MT Joghataei, T Mirzapour, M Bakhtiyari, M Shakeri, V Pirhajati, P Shirinbayan, M Koruji
International Journal of Nanomedicine 2013
Downregulation of cold-inducible RNA-binding protein activates mitogen-activated protein kinases and impairs spermatogenic function in mouse testes
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K Hwang, RC Walters, LI Lipshultz
Nature Reviews Urology 2011
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KC Lo, T Domes
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BP Hermann, M Sukhwani, J Salati, Y Sheng, T Chu, KE Orwig
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BT Phillips, K Gassei, KE Orwig
Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2010
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K Hwang, DJ Lamb
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K Fujita, A Tsujimura
Reproductive Medicine and Biology 2010
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Spermatogonial stem cells in higher primates: are there differences from those in rodents?
BP Hermann, M Sukhwani, MC Hansel, KE Orwig
Reproduction (Cambridge, England) 2009
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BP Hermann, M Sukhwani, DR Simorangkir, T Chu, TM Plant, KE Orwig
Human reproduction (Oxford, England) 2009
Production of donor-derived sperm after spermatogonial stem cell transplantation in the dog
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Reproduction (Cambridge, England) 2008
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H Kubota, RL Brinster
Nature Clinical Practice Endocrinology & Metabolism 2006
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S Schlatt, A Honaramooz, J Ehmcke, PJ Goebell, H Rübben, R Dhir, I Dobrinski, P Patrizio
Human Reproduction 2005

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