Pre–B cell colony-enhancing factor (PBEF) is a highly conserved 52-kDa protein, originally identified as a growth factor for early stage B cells. We show here that PBEF is also upregulated in neutrophils by IL-1β and functions as a novel inhibitor of apoptosis in response to a variety of inflammatory stimuli. Induction of PBEF occurs 5–10 hours after LPS exposure. Prevention of PBEF translation with an antisense oligonucleotide completely abrogates the inhibitory effects of LPS, IL-1, GM-CSF, IL-8, and TNF-α on neutrophil apoptosis. Immunoreactive PBEF is detectable in culture supernatants from LPS-stimulated neutrophils, and a recombinant PBEF fusion protein inhibits neutrophil apoptosis. PBEF is also expressed in neutrophils from critically ill patients with sepsis in whom rates of apoptosis are profoundly delayed. Expression occurs at higher levels than those seen in experimental inflammation, and a PBEF antisense oligonucleotide significantly restores the normal kinetics of apoptosis in septic polymorphonuclear neutrophils. Inhibition of apoptosis by PBEF is associated with reduced activity of caspases-8 and -3, but not caspase-9. These data identify PBEF as a novel inflammatory cytokine that plays a requisite role in the delayed neutrophil apoptosis of clinical and experimental sepsis.
Song Hui Jia, Yue Li, Jean Parodo, Andras Kapus, Lingzhi Fan, Ori D. Rotstein, John C. Marshall
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Pulmonary surfactant: an immunological perspective
ZC Chroneos, Z Sever-Chroneos, VL Shepherd |
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | 2009 |
Dysregulation of maternal serum adiponectin in preterm labor
S Mazaki-Tovi, R Romero, E Vaisbuch, O Erez, P Mittal, T Chaiworapongsa, SK Kim, P Pacora, L Yeo, F Gotsch, Z Dong, CL Nhan-Chang, C Jodicke, BH Yoon, SS Hassan, JP Kusanovic |
Journal of Maternal-Fetal and Neonatal Medicine | 2009 |
Could alterations in maternal plasma visfatin concentration participate in the phenotype definition of preeclampsia and SGA?
S Mazaki-Tovi, R Romero, SK Kim, E Vaisbuch, JP Kusanovic, O Erez, T Chaiworapongsa, F Gotsch, P Mittal, CL Nhan-Chang, NG Than, R Gomez, JK Nien, SS Edwin, P Pacora, L Yeo, SS Hassan |
Journal of Maternal-Fetal and Neonatal Medicine | 2009 |
Differential Intra-abdominal Adipose Tissue Profiling in Obese, Insulin-resistant Women
A Liu, T McLaughlin, T Liu, A Sherman, G Yee, F Abbasi, C Lamendola, J Morton, SW Cushman, GM Reaven, PS Tsao |
Obesity Surgery | 2009 |
Nampt: linking NAD biology, metabolism and cancer
A Garten, S Petzold, A Körner, SI Imai, W Kiess |
Trends in endocrinology and metabolism: TEM | 2009 |
Genetic polymorphisms associated with acute lung injury
AJ Reddy, SR Kleeberger |
Pharmacogenomics | 2009 |
Adipocytokines are associated with radiographic joint damage in rheumatoid arthritis
YH Rho, J Solus, T Sokka, A Oeser, CP Chung, T Gebretsadik, A Shintani, T Pincus, CM Stein |
Arthritis & Rheumatism | 2009 |
Intracellular NAD levels regulate tumor necrosis factor protein synthesis in a sirtuin-dependent manner
FV Gool, M Gallí, C Gueydan, V Kruys, PP Prevot, A Bedalov, R Mostoslavsky, FW Alt, TD Smedt, O Leo |
Nature Medicine | 2009 |
NAMPT is essential for the G-CSF–induced myeloid differentiation via a NAD+–sirtuin-1–dependent pathway
J Skokowa, D Lan, BK Thakur, F Wang, K Gupta, G Cario, AM Brechlin, A Schambach, L Hinrichsen, G Meyer, M Gaestel, M Stanulla, Q Tong, K Welte |
Nature Medicine | 2009 |
Maternal visfatin concentration in normal pregnancy
S Mazaki-Tovi, R Romero, JP Kusanovic, E Vaisbuch, O Erez, NG Than, T Chaiworapongsa, CL Nhan-Chang, P Pacora, F Gotsch, L Yeo, SK Kim, SS Edwin, SS Hassan, P Mittal |
Journal of Perinatal Medicine | 2009 |
Visfatin in human pregnancy: maternal gestational diabetes vis-à-vis neonatal birthweight
S Mazaki-Tovi, R Romero, JP Kusanovic, E Vaisbuch, O Erez, NG Than, T Chaiworapongsa, CL Nhan-Chang, P Pacora, F Gotsch, L Yeo, SK Kim, SS Edwin, SS Hassan, P Mittal |
Journal of Perinatal Medicine | 2009 |
Lack of direct insulin-like action of visfatin/Nampt/PBEF1 in human adipocytes
E Wanecq, D Prévot, C Carpéné |
Journal of Physiology and Biochemistry | 2009 |
Retinol binding protein 4: An adipokine associated with intra-amniotic infection/inflammation
E Vaisbuch, S Mazaki-Tovi, JP Kusanovic, O Erez, NG Than, SK Kim, Z Dong, F Gotsch, P Mittal, T Chaiworapongsa, P Pacora, L Yeo, SS Hassan, R Romero |
Journal of Maternal-Fetal and Neonatal Medicine | 2009 |
Maternal plasma visfatin in preterm labor
S Mazaki-Tovi, R Romero, E Vaisbuch, O Erez, T Chaiworapongsa, P Mittal, SK Kim, P Pacora, F Gotsch, Z Dong, SS Hassan, JP Kusanovic |
Journal of Maternal-Fetal and Neonatal Medicine | 2009 |
Regulation of Inflammatory Cytokine Expression in Pulmonary Epithelial Cells by Pre-B-cell Colony-enhancing Factor via a Nonenzymatic and AP-1-dependent Mechanism
P Liu, H Li, J Cepeda, Y Xia, JA Kempf, H Ye, LQ Zhang, SQ Ye |
The Journal of biological chemistry | 2009 |
New Concepts and Emerging Issues in Sepsis
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2009 | |
Poly(ADP-ribose) polymerase 1 at the crossroad of metabolic stress and inflammation in aging.
Altmeyer M, Hottiger MO |
Aging | 2009 |
Nicotinamide phosphoribosyltransferase (Nampt): a link between NAD biology, metabolism, and diseases.
Imai S |
Current pharmaceutical design | 2009 |
Therapeutic potential of SIRT1 and NAMPT-mediated NAD biosynthesis in type 2 diabetes.
Imai S, Kiess W |
2009 |