(A) Disease course of a patient with anti-nephrin–associated MCD. Albuminuria given as urinary albumin-to-creatinine ratio (g/g). PLEX, plasma exchange; CsA, cyclopsporine A; Rtx, rituximab. (B) Representative periodic acid-Schiff (PAS) staining (left) and electron microscopy (EM, right) of the patient. Scale bars: 50 μm (PAS) and 1 μm (EM). (C) Western blot of immunoprecipitates (human (hu), mouse (ms), rabbit (rb), rat, guinea pig (gp), and swine (sw) glomerular extracts with serum from the patient (P) or from a healthy control (HC) individual). (D) Coomassie-blue stainings of urine samples from rabbits before (day –1) and after (days 3 and 5) the transfer of human IgG containing anti-nephrin autoantibodies or control IgG. (E) PAS stainings (left) and EM images (right) of rabbits 5 days after IgG transfer. Scale bars: 50 μm (PAS) and 500 μm (EM). (F) Representative immunofluorescence stainings for human IgG (hIgG) in colocalization with collagen IV (left), complement C3 in colocalization with wheat germ agglutinin (WGA, middle), and rabbit IgG (rbIgG) in colocalization with collagen IV (right) of kidneys from rabbits 5 days after IgG transfer. Panels are enlargements of the boxed areas in Supplemental Figure 2C. Scale bars: 5 μm.(G) Western blot of rabbit glomerular extracts (left) and of immunoprecipitates (rabbit glomerular extracts and anti-phosphotyrosine antibody,right). (H) Relative signal intensity given as the ratio of tyrosine-phosphorylated nephrin and input nephrin signal in (G). (I) Western blot of rabbit glomerular extracts (left) and of rabbit nephrin precipitated by human IgG present in glomerular extracts (right).