Representative NAD(P)H oscillations in adherent neutrophils from nonpregnant (a–e) and pregnant (f–j) women. Cells were untreated (a and f) or treated with LPS (b and g), LPS plus 6-AN (c and h), LPS plus colchicine (d and i), or LPS plus colchicine and 6-AN (e and j). The amplitudes of the NAD(P)H oscillations are higher in pregnancy cells (a and f). LPS causes higher-frequency oscillations in cells from nonpregnant women (b), but not in cells from pregnant women (g), which appears to be due to an inability to properly engage the HMS, as suggested by the inhibitor 6-AN (c and h). However, colchicine is able to restore the normal high-frequency oscillations to pregnancy cells (i) in a 6-AN–sensitive manner, suggesting that the ability to engage normal NAD(P)H flux has been restored. See Table 1 for quantitative results. Bar = 10 seconds.