Localization of G-6-PDase activity in neutrophils. Representative cells from nonpregnant women (a and b) and pregnant women (c and d) are shown. Cells were incubated in the absence (a and c) and presence (b and d) of LPS (50 ng/ml, 20 minutes). Cells were fixed and processed as described previously (22, 41). G-6-PDase activity is peripherally located in control cells (a and b) but was found to be enhanced near the centrosome in cells from pregnant women (c and d). n = 4. ×640.