Effect of anti-CD3 mAb on Na⁺ absorption and anion secretion across murine jejunum. Mice were injected with PBS (Sham-injected) or 0.2 mg anti-CD3 mAb 3 hours before sacrifice. Jejunal sections were mounted in Ussing chambers for measurement of I_sc and ²²Na flux measurements. (a) Mucosal-to-serosal ²²Na flux sensitive to 100 μM EIPA (n = 10 sections from six mice). (b) I_sc response to addition of 10 mM glucose in mucosal bath (15 minutes) to stimulate Na⁺-coupled glucose transport (n = 12 sections from six mice). (c) I_sc response to addition of 10 μM forskolin in bathing solutions (10 minutes) to stimulate cAMP-dependent anion secretion (n = 11 sections from six mice). Values are means ± SE. *P < 0.05, **P < 0.1 vs. sham-injected.