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Increased production of IL-7 uncouples bone formation from bone resorption during estrogen deficiency
M. Neale Weitzmann, … , Louise Weitzmann, Roberto Pacifici
M. Neale Weitzmann, … , Louise Weitzmann, Roberto Pacifici
Published December 1, 2002
Citation Information: J Clin Invest. 2002;110(11):1643-1650. https://doi.org/10.1172/JCI15687.
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Article Bone biology

Increased production of IL-7 uncouples bone formation from bone resorption during estrogen deficiency

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Abstract

Research Article

Authors

M. Neale Weitzmann, Cristiana Roggia, Gianluca Toraldo, Louise Weitzmann, Roberto Pacifici

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Figure 6

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IL-7 inhibits OPG production in ROS 17/2.8 cells. (a) Using semiquantita...
IL-7 inhibits OPG production in ROS 17/2.8 cells. (a) Using semiquantitative RT-PCR, we measured OPG mRNA at 24 hours in ROS 17/2.8 cells stimulated with vehicle (PBS plus 0.1% BSA) or IL-7 (10 ng/ml). Data are representative of two independent experiments. (b) OPG mRNA bands in a were quantitated by densitometry and normalized against GAPDH. (c) OPG protein production was measured by Western blot at 24 hours in primary calvarial OBs stimulated with vehicle (PBS plus 0.1% BSA) or IL-7 (10 ng/ml). Data are representative of two independent experiments. (d) OPG protein concentrations in c were quantitated by densitometry.

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