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Neutrophil-independent mechanisms of caspase-1– and IL-18–mediated ischemic acute tubular necrosis in mice
Vyacheslav Y. Melnikov, … , Danica Ljubanovic, Charles L. Edelstein
Vyacheslav Y. Melnikov, … , Danica Ljubanovic, Charles L. Edelstein
Published October 15, 2002
Citation Information: J Clin Invest. 2002;110(8):1083-1091. https://doi.org/10.1172/JCI15623.
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Article Cell biology

Neutrophil-independent mechanisms of caspase-1– and IL-18–mediated ischemic acute tubular necrosis in mice

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Abstract

Research Article

Authors

Vyacheslav Y. Melnikov, Sarah Faubel, Britta Siegmund, M. Scott Lucia, Danica Ljubanovic, Charles L. Edelstein

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Figure 6

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Caspase-1 activity (a), IL-18 protein (b), neutrophil infiltration (c), ...
Caspase-1 activity (a), IL-18 protein (b), neutrophil infiltration (c), and active form of IL-18 (d) in the kidney in neutrophil-depleted mice with ischemic ARF. (a) Caspase-1 activity increased in both vehicle-treated (neutro+) and neutrophil-depleted (neutro–) mice with ARF. *P < 0.001 vs. sham; **P < 0.001 vs. sham, NS vs. neutro+; n = 5. (b) IL-18 protein was measured by the ECL assay that detects both pro–IL-18 and active IL-18. IL-18 was increased in ischemic ARF in neutro+ as well as neutro– mice with ischemic ARF compared with sham-operated controls. IL-18 was higher in neutro+ mice than in neutro– mice. *P < 0.001 vs. sham; **P < 0.01 vs. sham, P < 0.01 vs. neutro+; n = 11. (c) Neutrophil infiltration (neutrophils/mm2) was increased in ischemic ARF in neutro+ and prevented in neutro– mice with ischemic ARF. *P < 0.01 vs. sham; **P < 0.01 vs. vehicle-treated ARF, NS vs. sham; n = 7. (d) There was no difference in the amount of active IL-18 protein (18 kDa) on immunoblot analysis in whole-kidney homogenates in neutro+ versus neutro– mice with ischemic ARF. Recombinant murine IL-18 (PeproTech Inc., Rocky Hill, New Jersey, USA) was used as a positive control (Pos). A representative picture of three separate experiments is shown.

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