TNF-α dependence of leukocyte infiltration in cisplatin nephrotoxicity. Sections of kidney harvested 72 hours after injection were stained for leukocytes as described in Methods. Thirty ×40 fields of kidney cortex were examined from each animal. The total number of leukocytes in those 30 fields is presented in the figure. *P < 0.02 vs. saline or cisplatin plus GM6001. n = 2–7. WT, wild type; KO, knockout.