Effect of cisplatin on kidney morphology in wild-type and TNF-α knockout mice. (a and b) Wild-type mice exhibit severe tubular necrosis, tubular dilation, and cast formation throughout the cortex. (c and d) TNF-α knockout mice have well-preserved renal morphology. Kidneys were removed 72 hours after injection with cisplatin. Sections were stained with PAS and photographed at ×100 magnification (a and c) or ×400 magnification (b and d).